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  • Hosted by:Chinese Academy of Sciences
    Sponsored by:Institute of Botany, Chinese Academy of Sciences, Botanical Society of China
    Co-hosted by:Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences
    Institute of Biotechnology and Germplasm Resources, Yunnan AgriculturalAcademy
    Fujian Agriculture and Forestry University
    Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University
    State Key Laboratory of Crops Biology, Shandong Agricultural University

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Pull-down and Co-immunoprecipitation Assays of Interacting Proteins in Plants
Chongyi Xu
Chinese Bulletin of Botany    2020, 55 (1): 62-68.   DOI: 10.11983/CBB19143
Accepted: 24 September 2019

Abstract6717)   HTML289)    PDF (3878KB)(6383)       Save

Protein-protein interactions play a key role in cellular signaling, involved in various biological processes. Studies on these interactions are therefore crucial toward understanding the regulatory networks of cellular signaling. It is a standard practice that the protein-protein interactions identified by the yeast two-hybrid system should be independently confirmed by in vitro and in vivo approaches. Pull-down and co-immunoprecipitation (Co-IP) are routine approaches to detect protein-protein interactions. Pull-down assay is used to detect direct or physical interactions between proteins in vitro. In plant biology studies, one of the most convenient methods to detect protein-protein interactions is the transient expression of the target proteins in Nicotiana benthamiana leaves followed by the Co-IP assay. In this paper, we describe the principles and protocols for the GST tag-based pull-down assay and the Co-IP assay of proteins transiently expressed in N. benthamiana leaves, providing a reference for detecting plant protein-protein interactions.

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Cited: CSCD(1)
Luciferase Complementation Assay for Detecting Protein Interactions
Yan Zhao,Jianmin Zhou
Chinese Bulletin of Botany    2020, 55 (1): 69-75.   DOI: 10.11983/CBB19229
Accepted: 04 December 2019

Abstract5409)   HTML245)    PDF (1554KB)(4028)       Save

Protein-Protein interactions play important roles in various eukaryotic biological processes. Compared to other techniques measuring protein-protein interactions in plants, the Luciferase Complementation Assay (LCA), based on Agrobacterium-mediated transient expression in Nicotiana benthamiana, is a simple, sensitive, reliable, highly quantitative and low background method that can be easily scaled up for high-throughput interactome studies. Here, we describe a protocol that includes two alternative data collection methods to qualitative and quantitative analyse luminescence or luminous intensity to detect protein-protein interactions in plant cells.

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An Effective Method for the Rooting of Tea Cuttings
Xiaomei Liu,Lili Sun,Xiangdong Fu,Hong Liao
Chinese Bulletin of Botany    2019, 54 (4): 531-538.   DOI: 10.11983/CBB19025
Accepted: 29 April 2019

Abstract3757)   HTML69)    PDF (1606KB)(528)       Save

Tea (Camellia sinensis) is one of the most important beverage crops in the world. With the expanding cultivation area, the demand for tea seedlings is increasing. However, there are many problems with the traditional breeding method for tea plants using cuttings, such as low rooting rate, time consumption and difficulties to obtain materials. Therefore, optimizing the cutting method is of great importance for tea production. In this study, we first changed the culture medium to sponges and found that tea cuttings were able to generate new roots within 1 month on sponges, with rooting rate 32.2%. Second, we optimized the cutting materials by using fresh green tea branches in sponges, and the rooting potential of goung branch maintained with one bud and one leaf is better. In addition, we found that supplying rooting powder to sponges significantly promoted callus formation and new root generation from cuttings. In general, the most effective way was to apply 1.25 g∙L -1 rooting powder to cuttings for 48 h, for a rooting rate of 42.0%. We have established an effective rooting method for tea cuttings by optimizing the culture medium, cutting materials and adding optimal rooting powder. This method could shorten the rooting time, avoid the restriction of cutting materials, and thus effectively reduce the expense of tea cuttings, which has application prospects in tea production.

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Analysis of Nutritive Components of Physalis pubescens and Its Utilization Value
Li Hong-en and Zhang Jian-xin
Chinese Bulletin of Botany    1988, 5 (04): 240-242.  
Abstract3588)      PDF (1649KB)(427)       Save
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Achievements and Advance in Chinese Plant Sciences in 2019
Jianru Zuo, Xiaoquan Qi, Rongcheng Lin, Qian Qian, Hongya Gu, Fan Chen, Shuhua Yang, Zhiduan Chen, Yongfei Bai, Lei Wang, Xiaojing Wang, Liwen Jiang, Langtao Xiao, Kang Chong, Tai Wang
Chinese Bulletin of Botany    2020, 55 (3): 257-269.   DOI: 10.11983/CBB20108
Accepted: 05 June 2020

Abstract3549)   HTML184)    PDF (912KB)(1979)       Save

Chinese researchers in plant sciences published more original papers in international top journals and mainstream journals of plant science than last year, and made remarkable achievements in several areas. Research on the supramolecular structure and function of diatom photosynthetic membrane proteins was selected in the top 10 achievements in Chinese Sciences in 2019 and the top achievements of Chinese Life Sciences in 2019. Research on the structure and function of plant disease-resistant bodies was selected in the top 10 achievements of Chinese Life Sciences in 2019. In this review, we provide a commentary on the significant progress made by Chinese researchers in plant sciences this year.

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Cited: CSCD(1)
Research Advances in Plant Science in China in 2017
Chen Fan, Qian Qian, Wang Tai, Dong Aiwu, Qi Xiaoquan, Zuo Jianru, Yang Shuhua, Lin Rongcheng, Xiao Langtao, Gu Hongya, Chen Zhiduan, Jiang Liwen, Bai Yongfei, Kong Hongzhi, Chong Kang
Chinese Bulletin of Botany    2018, 53 (4): 391-440.   DOI: 10.11983/CBB18177
Abstract3502)   HTML99)    PDF (1295KB)(4264)       Save

Plant science in China continued to maintain high-speed progress in 2017, with frequent remarkable achieve- ments and a steady increase in the number of original papers published in international top journals. Researchers in plant science in China have made brilliant achievements, such as the discovery of new broad-spectrum disease resistance mechanisms, the genetic basis and mechanism of rice broad-spectrum disease resistance, and the mechanism of Phytophthora infestation. Two achievements were included in the “Breakthrough of the year: The top 10 scientific achievements of life science in China in 2017”. Rice biology, evolution and genomics and hormone biology were highlighted. Also, academician Li Jiayang, who researches the molecular network of higher plants and metabolic pathway as well as rice design breeding, won first prize of National Natural Science in 2017 for his research "Molecular Mechanisms and Variety Design of High Yield and Quality Characters of Rice". This groundbreaking contribution with significant international impact marks the leading position of Chinese plant science in the international scientific frontier of this field. In this review, we give an overview of the significant progress made in plant science in China in 2017, review the latest findings and hot events in plant science in 2017, and share the great achievements made by Chinese scientists.

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Cited: CSCD(4)
Research Advances in Plant Science in China in 2018
Qian Qian, Xiaoquan Qi, Rongcheng Lin, Shuhua Yang, Aiwu Dong, Jianru Zuo, Fan Chen, Langtao Xiao, Hongya Gu, Zhiduan Chen, Yongfei Bai, Xiaojing Wang, Lei Wang, Liwen Jiang, Kang Chong, Tai Wang
Chinese Bulletin of Botany    2019, 54 (4): 405-440.   DOI: 10.11983/CBB19165
Accepted: 28 August 2019

Abstract3491)   HTML142)    PDF (2396KB)(3077)       Save

Plant science in China continues on the track of rapid development in 2018, with many remarkable achievements and a marked increase in number of original papers published in international top journals. The achivement “regulating the plant growth-metabolism balance to achieve sustainable agricultural development” was selected in the top ten progress of Chinese science in 2018, and “the history of angiosperm flora evolution in China” in the top ten progress of Chinese life science in 2018. Studies in rice and fruit and vegetable fields has been internationally leading. In this review, we summarize the significant progress in Chinese plant science in 2018, review the latest findings and hot events, and share the great achievements made by Chinese scientists.

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The ABC Model and the Quartet Model of Floral Organ Identity
LIU Jian-Wu SUN Cheng-Hua LIU Ning
Chinese Bulletin of Botany    2004, 21 (03): 346-351.  
Abstract3428)      PDF (209KB)(4734)       Save
The ABC model was established in late 1980s to explain the genetic interactions between floral homeotic mutations. As the progress in flower developmental genetics, the ABC model was expanded to the ABCD model with the introduction of D class genes for ovary identity. More recently, a A-E model was proposed and will be discussed in this short review.
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Cited: Baidu(57) CSCD(9)
In Vitro Ubiquitination Assay for Plant Proteins
Qingzhen Zhao,Lijing Liu,Qi Xie,Feifei Yu
Chinese Bulletin of Botany    2019, 54 (6): 764-772.   DOI: 10.11983/CBB19152
Accepted: 31 October 2019

Abstract3299)   HTML52)    PDF (3367KB)(1178)       Save

Ubiquitin activating enzyme (E1), ubiquitin conjugating enzyme (E2) and ubiquitin protein ligase (E3) are the key enzymes of ubiquitin modification of substrate proteins. There are large amounts of genes encoding these ubiquitination enzymes in all eukaryotic genomes. Analyzing the biochemical characteristics and specificity of these enzymes and their substrate proteins is important for their functional study. Here we describe a simple and fast method for in vitro ubiquitination assay. In the presence of E1 and ubiquitin, E2 activity can be determined by detecting the DTT-sensitive thio-ester formation. The E3 activity of a putative protein as well as the E2-E3 or E3-substrate specificities can also be explored by in vitro ubiquitination assay. This system is mainiy based on proteins from Arabidopsis, which includes most varieties of Arabidopsis E2 proteins that are tested with several RING-finger type E3 ligases. This system facilitate not only the exploration of E3 activity in combination with various Arabidopsis E2 members but also the study of E2-RING E3 and RING E3-substrate specificities. This system is suitable for the ubiquitination assays of eukaryotic proteins, especially for plant proteins.

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Cited: CSCD(1)
The Properties, Functions, Catalytic Mechanism and Applicability of Laccase
WANG Guo-Dong CHEN Xiao-Ya
Chinese Bulletin of Botany    2003, 20 (04): 469-475.  
Abstract3235)      PDF (466KB)(1573)       Save
Laccase belongs to the family of multicopper oxidases. In this review, the molecular structure, substrate specificity, catalytic mechanism and other physicochemical parameters of laccase are summarized. The role of laccase in plant cell wall formation and pathogen virulence are discussed. For applications, we pay special attention to the potential of laccase in bioremediation.
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Cited: Baidu(40)
Protocols for Analyzing Plant Phospho-proteins
Dan Zhu,Hanwei Cao,Yuan Li,Dongtao Ren
Chinese Bulletin of Botany    2020, 55 (1): 76-82.   DOI: 10.11983/CBB19208
Accepted: 03 January 2020

Abstract3185)   HTML145)    PDF (4000KB)(3737)       Save

Protein phosphorylation is one of the important protein posttranslational modifications that is involved in the regulation of most cellular processes in plants. Protein kinases catalyze the phosphorylation by transferring the phosphate group in ATP to the substrate proteins. The phosphate is usually covalently linked to the hydroxyl group of specific amino acid residues in the substrates by an ester bond. The mostly studied phosphorylation sites are serine, threonine, and tyrosine residues. Here, we present protocols and related tips for the in vitro and in vivo protein phosphorylation assays.

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Cited: CSCD(1)
Sporangia Morphology of Ferns I. Lindsaeaceae
Xile Zhou, Dongmei Jin, Yicheng Liu, Hui Shang, Yuehong Yan
Chinese Bulletin of Botany    2017, 52 (3): 322-330.   DOI: 10.11983/CBB16104
Abstract3093)   HTML38)    PDF (509KB)(2290)       Save

Sporangia are propagative organs of ferns and their morphology has great significance for fern taxonomy and phylogeny. In this study, we used sodium hypochlorite solution to observe fern sporangia. By this process, we could obtain sporangia photos under light microscopy. We studied the sporangia morphology of 13 species belonging to four genera of the fern family Lindsaeaceae and found that the shape of the capsule is ellipsoidal, with a vertical annulus; the pedicel is composed of three rows of cells. The cell number annulus is reduced in Odontosoria, Osmolindsaea, Tapeinidium and Lindsaea, whereas the volume of capsule and cell number of stomium and capsule are reduced in Odontosoria, Tapeinidium, Osmolindsaea and Lindsaea. As well, the same genus shows more differences between species, such as Odontosoria biflora and O. chinensis as well as Osmolindsaea odorata and Os. japonica, but less difference between the genera of Tapeinidium and Lindsaea. Studies of sporangia morphology will be useful for further research in other groups of ferns.

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A Modified CTAB Protocol for Plant DNA Extraction
Jinlu Li, Shuo Wang, Jing Yu, Ling Wang, Shiliang Zhou
Chinese Bulletin of Botany    2013, 48 (1): 72-78.   DOI: 10.3724/SP.J.1259.2013.00072
Abstract2991)      PDF (712KB)(3914)       Save
It is very important but usually difficult to extract high quality DNA from plants for molecular work since there exist a great deal of polysaccharides, hydroxybenzenes, esters and other secondary metabolities. In this paper we provide a simple modified CTAB (mCTAB) protocol for extracting plant DNA. The mCTAB method protocol includes 18 steps. (1) Weigh ca. 20 mg of dry plant tissue and ground into powder with sand using a mortar or a pestle. Remove the powder into a 2.0 mL microcentrifuge tube. (2) Add 1.0 mL pre-cooled buffer A (Table 2) to the tube, mix well and incubate the tube on ice for 15 min. Mix sample 2–3 times during incubation by inverting the tube. (3) Centrifuge the tube at 7 000 ×g for 10 min. Discard the supernatant liquid by pouring it out of the tube. (4) Repeat step 2 and 3 until the supernatant is not viscous. (5) Add 0.7 mL buffer B (Table 3), mix well and incubate at 65°C for 90–120 min. Mix the sample several times during incubation by inverting the tube. (6) Centrifuge at 10 000 ×g for 10 min, remove the supernatant to a new microcentrifuge tube. The precipitate is reusable from step 5 if necessary. (7) Add 0.7 mL CI (chloroform: isoamyl alcohol=24:1, v/v), mix it well for 10 min by inverting tube gently. (8) Centrifuge at 10 000 ×g, for 10 min, carefully remove the supernatant to a new 1.5 mL microcentrifuge tube. (9) Repeat step 7 and 8 until no precipitate appearing between the two layers of liquid after centrifuging. (10) Add 0.5 mL pre-cooled isopropanol, carefully mix well . Incubate at –20°C for 20 min. (11) Centrifuge at 10 000 ×g for 10 min, discard the supernatant, centrifuge the tube briefly to collect the remaining liquid and remove it by pipetting. (12) Add 0.1 mL RNase (100 mg·L–1) and incubate at 37°C for 30–60 min. (13) Add 0.1 mL ddH2O, 0.1 mL 5 mol·L–1NaCl and 0.8 mL pre-cooled ethanol (95%), carefully mix well. (14) Centrifuge at 10 000 ×g for 10 min, discard the supernatant. (15) Add 0.5 mL 75% ethanol, re-suspend the pellet, centrifuge at 10 000 ×g for 2 min, discard the supernatant. (16) Repeat step 15. (17) Add 0.1 mL TE to dissolve DNA after ethanol has evaporated. (18) Estimate the concentration and the purity of the DNA solution. Store it at 4°C for immediate use, at –20°C for short time storage and –80°C for long time storage. We compared our protocol with four frequently used and commercially available kits. The result showed that our mCTAB method yielded much more DNA of high quality that is suitable for PCR amplification but with much lower cost.
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Cited: CSCD(61)
A Discussion on Chlorophyll Fluorescence Kinetics Parameters and Their Significance
ZHANG Shou-Ren
Chinese Bulletin of Botany    1999, 16 (04): 444-448.  
Abstract2958)      PDF (215KB)(1319)       Save
The chlorophyll fluorescence kinetics technique is referred to as a quick and nonintrusive probe in the studies of plant photosynthetic function. But there are irregularity and confusion in the nomenclature and interpretation of the parameters. In this paper we discuss the problems and try to solve them.
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Cited: Baidu(1985) CSCD(899)
SUMOylation and Its Biological Function in Plants
Panglian Xu;Mianwei Zeng;Lixia Huang;Chengwei Yang*
Chinese Bulletin of Botany    2008, 25 (05): 608-615.  
Abstract2942)      PDF (166KB)(1944)       Save
Post-translational modification by small ubiquitin-related modifiers (SUMOs) is an important regulatory process to modulate protein function. This paper summarizes the SUMOylation pathway in plants; the pathway consists of SUMO molecules, a SUMO conjugation enzyme cascade and de-conjugation enzymes. Nascent SUMOs are processed by SUMO-specific proteases, then mature SUMOs are conjugated to substrate proteins by sequential action of three groups of enzymes: SUMO-activating enzymes (E1), SUMO-conjugating enzymes (E2) and SUMO-ligating enzymes (E3). SUMOylation can be reversed by SUMO-specific proteases. SUMO modification in plants is involved in flowering induction, hormone signaling, pathogen defense and stress response.
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Cited: Baidu(10) CSCD(7)
An Overview of Genome-wide Association Studies in Plants
Yuhui Zhao, Xiuxiu Li, Zhuo Chen, Hongwei Lu, Yucheng Liu, Zhifang Zhang, Chengzhi Liang
Chinese Bulletin of Botany    2020, 55 (6): 715-732.   DOI: 10.11983/CBB20091
Accepted: 26 August 2020

Abstract2907)   HTML159)    PDF (1798KB)(3043)       Save

Genome-wide association study (GWAS) is a general approach for unraveling genetic variations associated with complex traits in both animals and plants. The development of high-throughput genotyping has greatly boosted the development and application of GWAS. GWAS is not only used to identify genes/loci contributing to specific traits from diversenatural populations with high-resolution genome-wide markers, it also systematically reveals the genetic architecture underlying complex traits. During recent years, GWAS has successfully detected a large number of QTLs and candidate genes associated with various traits in plants including Arabidopsis, rice, wheat, soybean and maize. All these findings provided candidate genes controlling the traits and theoretical basis for breeding of high-yield and high-quality varieties. Here we review the methods, the factors affecting the power, and a data analysis pipeline of GWAS to provide reference for relevant research.

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Cited: CSCD(2)
TDZ: An Efficacious Plant Growth Regulator
XU Xiao-Feng HUANG Xue-Lin
Chinese Bulletin of Botany    2003, 20 (02): 227-237.  
Abstract2902)      PDF (436KB)(2303)       Save
TDZ(N-phenyl-N’-1,2,3-thidiazol-5-yl-urea) is a substituted phenylurea compound and has emerged as a highly efficacious bioregulant of morphogenesis in the tissue culture of many plant species. Application of TDZ induces a diverse array of cultural responses ranging from induction of callus to formation of somatic embryos.TDZ exhibits the unique property of mimicking both auxin and cytokinin effects on growth and differentiation of cultured explants. The recent app roaches applied to study the morphogenic events initiated by TDZ are clearly beginning to reveal the details of a variety of underlying mechanisms. Various reports indicate that TDZ may act through modulation of the endogenous plant growth substances, or as a result of induced stress. The other possibilities include the modification in cell membranes, energy levels, nutrient uptake, or nutrient assimilation. In this review, several of these possibilities are presented and summarized in light of recently published studies on characterization of TDZ-induce dmorphogenic effects.
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Cited: Baidu(106) CSCD(87)
Structure and Function of Plant Plasma Membrane H+-ATPase
QIU Quan-Sheng
Chinese Bulletin of Botany    1999, 16 (02): 122-126.  
Abstract2864)      PDF (227KB)(955)       Save
Plant plasma membrane H+ -ATPase was a P-type proton pump. The transmembrane electrochemical gradients generated by the enzyme was the primary force for the transmembrane transports. Researches indicated that the plasma membrane H+ -ATPase plays important roles in the growth and development in plants. It was called the "master enzyme" in plant cells; Great progress had been made about the biochemical character, gene expression and regulation, structure and function of the H+ -ATPase in recent years. In this article the biochemical character, molecular structure, regulatory mechanism and physiological roles of the plasma membrane H+ -ATPase were reviewed.
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Cited: Baidu(13) CSCD(12)
Sensibility of Monocotyledon and Dicotyledon to Copper and the Accumulat and the Distribution of Copper In the Plants
Liu Wen-zhang and Sun Dian-lan
Chinese Bulletin of Botany    1985, 3 (05): 19-21.  
Abstract2822)      PDF (2733KB)(1164)       Save
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Responses of the Morphological and Anatomical Structure of the Plant Leaf to Environmental Change
Chinese Bulletin of Botany    2005, 22 (增刊): 118-127.  
Abstract2786)      PDF (62KB)(3329)       Save
In the evolution of plants, the leaf is more sensitive and plastic to environmental change than other organs; environment change usually results in morphological and anatomical responses of the leaf, including morphology (length, width, thickness), surface (stomata, epidermis, attachment) and mesophyll (palisade, spongy, intercellur space, sclerified, vein). This review describes the above-mentioned adaptive characters of terrestrial plant leaves to alterations in environmental factors such as water, temperature, light and CO2 concentration and combined effects, and analyzes recent research, then indicates the emphasis and direction of future study.
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Cited: Baidu(170)
Regulation of Expression and Functions of the Heat Shock Proteins of Plant
HUANG Xiang-Fu;HUANG Shang-Zhi and FU Jia-Rui
Chinese Bulletin of Botany    1999, 16 (05): 530-536.  
Abstract2778)      PDF (465KB)(919)       Save
All organism respond to elevated temperatures and many other stresses with the production of a defined set of proteins called heat shock proteins ( HSPs). The basic characteristics of HSPs are highly conserved, diversed and the heat shock response is temporal. Although HSPs were first characterized because their expression increased in response to elevated temperature, some HSPs are found at sighificant levels in other stresses, in normal, nonstressed cells and produced at particular stages of cell cycle or during development in the absence of stresses. The findings indicated that HSPs have many functions, including molecular chaperones, thermotolerance, chilling tolerance, a special role in the development of organisms and other biochemical functions in cell metabolism, The association of HSF with HSP70 may be important in the regulation of heat shock gene transcriptional activity. The expression of heat shock gene is self-regulated by HSP.
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Cited: Baidu(29) CSCD(26)
Plant Retrotransposons and Their Molecular Markers
WANG Zi-Cheng LI Zhong-Ai DENG Xui-Xin
Chinese Bulletin of Botany    2003, 20 (03): 287-294.  
Abstract2733)      PDF (541KB)(831)       Save
Retrotransposons are a class of eukaryotic transposable elements, consisting of the long terminal repeat (LTR) and non-LTR retrotransposons. Retrotransposons are ubiquitous in the plant kingdom by high copy number and can be transmitted between generations by vertical transmission and between species by horizontal transmission. The same family retrotransposons presented highly heterogeneous populations in all higher plant genomes. Many of the plant retrotransposons are transcriptionally activated by various biotic and abiotic stress factors. Retrotransposons are used as molecular markers for their traits. S-SAP, IRAP, REMAP and RBIP are developed and will be applied widely in gene mapping, genetic biodiversity and phylogeny studies, and cultivar certification.
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Cited: Baidu(17) CSCD(11)
Advances in Photoreceptor-mediated Signaling Transduction in Flowering Time Regulation
Chaofeng Ma,Silan Dai
Chinese Bulletin of Botany    2019, 54 (1): 9-22.   DOI: 10.11983/CBB18147
Abstract2688)   HTML44)    PDF (1616KB)(1514)       Save

Light is an important environmental factor that affects plant growth and development. Flowering is the most important event in higher plants. Plants perceive accurately changes in the surrounding light environments by photoreceptors, thus activating a series of signaling transduction processes and initiating flowering. Here, we summarized the current understanding of the structural characteristics and physiological functions of various photoreceptors in higher plants. We reviewed the molecular mechanisms of phytochromes, cryptochromes, and FKF1/ZTL/LKP2 in mediating signaling transduction and flowering time, including transcriptional and post-transcriptional regulation of CO and FT. Finally, we described the advances in photoreceptor-mediated-integration of light, temperature, and gibberellin signals in regulating flowering. Future directions in this area were also proposed.

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Cited: CSCD(6)
Research Advances in Plant Metabolomics
YIN Heng LI Shu-Guang BAI Xue-Fang DU Yu-Guang
Chinese Bulletin of Botany    2005, 22 (05): 532-540.  
Abstract2639)      PDF (54KB)(3603)       Save
The metabolome refers to all the low-molecular-weight metabolites present in an organism or cell in a particular physiological period. The term plant metabolomics is used for defining the technology of high-throughput, nonbiased analyses of the metabolome of plant extracts. Research into plant metabolomics has advanced greatly during recent years. This review introduces the definition, history and research approaches of plant metabolomics and gives several typical examples to elucidate the application of plant metabolomics.
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Cited: Baidu(12) CSCD(14)
The Physiological Role of Glutamate Dehydrogenase in Higher Plants
Chinese Bulletin of Botany    2001, 18 (04): 396-401.  
Abstract2595)            Save
Glutamate dehydrogenase(GDH) is present mainly in mitochondria in higher plants and catalyses both the amination of a-oxoglutamate, with NADH as the electron donor, and the deamination of glutamate to ammonia and a-oxoglutamate, with NAD+ as the electron receptor. The NAD (H)-GDH, with a molecular weight of 255-258kD, is composed of six subunits of a and b in different ratios to form seven isoenzymes. The enzyme seems to function in assimilation of ammonia under stress conditions such as high temperature, in senescence and other abnormalities. It also functions in higher plants to direct carbon skeletons into the citric acid cycle under conditions of carbon stress.
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Cited: Baidu(32) CSCD(18)
The Ecosystem Function of Biodiversity
LIU Feng;HE Jin-Sheng and CHEN Wei-Lie
Chinese Bulletin of Botany    1999, 16 (06): 671-676.  
Abstract2498)      PDF (1019KB)(572)       Save
This review is about the effects of biodiversity on ecosystem functions and services. It focuses on the following parts: 1. hypotheses about different roles of different species in the ecosystem and how biodiversity affects ecosystem functions; 2. relations between biodiversity and stability of ecosystem; 3. how the biodiversity affects production of ecosystem; 4. the relation between biodiversity and sustainability of the ecosystem. Several problems for further research are also pointed out.
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Cited: Baidu(48)
Role of Post-translational Modification of Proteins in ABA Signaling Transduction
Jing Zhang,Suiwen Hou
Chinese Bulletin of Botany    2019, 54 (3): 300-315.   DOI: 10.11983/CBB18217
Accepted: 16 January 2019

Abstract2474)   HTML79)    PDF (1731KB)(1676)       Save

Abscisic acid (ABA) plays a key role in the growth, development and stress condition of plants. The process of plant response to ABA is completed by signal recognition, transduction, and response cascades. The core ABA signaling pathway consists of receptor RCAR/PYR/PYLs, phosphatase PP2Cs, kinase SnRK2s, and transcription factors and ion channel proteins. Post-translational modifications (PTMs) of proteins such as phosphorylation, ubiquitination, small ubi- quitin-related modifier (SUMOylation) and redox modifications plays an important role in ABA signaling. This review focused on the role of modifications in the core ABA signaling pathway.

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Cited: CSCD(1)
Recent Advances in the Regulation Mechanism of Transcription Factors and Metabolic Engineering of Anthocyanins
Xuewei Song,Jiebing Wei,Shaokang Di,Yongzhen Pang
Chinese Bulletin of Botany    2019, 54 (1): 133-156.   DOI: 10.11983/CBB18016
Accepted: 10 December 2018

Abstract2463)   HTML38)    PDF (1130KB)(895)       Save

Anthocyanins are among the most important flavonoid compounds widely present in plants. Anthocyanins play significant roles in plant growth and development as well as human nutrition and health care. The anthocyanin biosynthetic pathway has been widely documented, and the anthocyanin metabolic regulation network is being constantly improved. The transcription factors that regulate anthocyanin biosynthesis mainly include three classes: MYB, bHLH and WD40 proteins. The proteins regulate the accumulation, location and levels of anthocyanins by activating or suppressing the expression of key structural genes, including CHS, ANS and DFR. This review briefly introduces the anthocyanin biosynthetic pathway and summarizes the molecular mechanism of transcriptional regulation based on recent progress. It mainly focuses on the molecular mechanism of MYB, bHLH and WD40 transcription factors in the regulation of anthocyanins in model plants. In addition, it summarizes the use of these transcription factors in anthocyanin metabolic engineering in ornamental plants and fruit crops. This review will provide valuable references for the in-depth investigation of transcriptional regulation and improving anthocyanins by metabolic engineering.

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Cited: CSCD(7)
Formation and Structural Change on Plasmodesmata of Plants
LI Ming-Yi
Chinese Bulletin of Botany    1998, 15 (增刊): 47-53.  
Abstract2459)      PDF (411KB)(511)       Save
Through the discussion of the origin, formation and mechanism of regulating material transportation of plasmodesmata, it shows that plasmodesmata plays an important role in material transportation and signal transduction. Plasmodesmal origin and secondary modification are compared between higher plant and lower plant also. Structurally modified plasmodesmata give rise to gigachannel (100-1000 nm) because of activation of enzymes.
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Research Advances in Auxin Biosynthesis
Jiali Wang, Dongcheng Liu, Xiaoli Guo, Aimin Zhang
Chinese Bulletin of Botany    DOI: 10.3724/SP.J.1259.2012.00292
Abstract2406)      PDF (407KB)(3438)       Save
Research Advances in Plant Science in China in 2016
Chinese Bulletin of Botany    2017, 52 (4): 394-452.   DOI: 10.11983/CBB17147
Abstract2396)   HTML36)    PDF (1659KB)(2738)       Save

Plant science in China continued to forge ahead in 2016, with a steady increase in number of original articles published in the top high-impact-factor international journals. Journals of Chinese plant science improved and entered the top three ranks of research journals in the world. Chinese scientists have made impressive and enviable achievements in many important field of plant science. The molecular genetics mechanism of heterosis in rice yield was included in the “Top 10 achievements in Chinese Science in 2016”. The “Breakthrough of the year: The top 10 scientific achievements of life science in China in 2016” included studies on signaling between male and female gametophytes during fertilization in plants and the perception mechanism of the strigolactones receptor. Plant science in China, especially in the field of crop research, represented by rice science, is occurring in the international arena. For example, a series of significant advance has been made in rice genome resources and technology platform, resequencing, clone of rice functional genes and analysis of regulatory network (revealing the mechanism of disinhibition activation of strigolactones signal transduction, interpreting sterility hybrid between indica and japonica at molecular level and the mechanism of action of wide compatibility gene S5, finding a genomic loci of controlling cold tolerance of rice), leading the scientific research of rice and even crops in the world. In this review, we comment on the significant progress made in plant science in China in 2016, review the latest findings and hot events in plant science in 2016, and share the great achievements made by Chinese scientists.

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The Significance and
Han Xing-guo Huang Jian hui;Guo Zhi-ping
Chinese Bulletin of Botany    1995, 12 (专辑2): 168-184.  
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The Illustrations in Which Show the Different Stage Characteristic of Spikelet Splitting of Winter Wheat
Cui Jing-mei and Ji Ling-fen
Chinese Bulletin of Botany    1985, 3 (04): 60-64.  
Abstract2379)      PDF (380KB)(1315)       Save
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A Brief Introduction to Life History of Porphyra
Zhou Yun-long
Chinese Bulletin of Botany    1985, 3 (02): 57-59.  
Abstract2378)      PDF (212KB)(891)       Save
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Physiological Function and Mechanism of Hydrogen Sulfide in Plants
Shang Yuting, Zhang Nina, Shangguan Zhouping, Chen Juan
Chinese Bulletin of Botany    2018, 53 (4): 565-574.   DOI: 10.11983/CBB17099
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Hydrogen sulfide (H2S) is the third gas signaling molecule after nitric oxide (NO) and carbon monoxide (CO) and is of great importance in many physiological activities in plants. H2S can promote plant photosynthesis, alleviate various stresses and promote plant growth and development. However, functional research of H2S in plants is relatively scarce. This article summarizes the latest research of the physicochemical properties, main physiological functions and mechanism of H2S and the interaction with other signaling molecules. Future prospects of H2S signaling are discussed.

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Halophytes in China
ZHAO Ke-Fu;LI Fa-Zeng;FAN Shou-Jin and FENG Li-Tian
Chinese Bulletin of Botany    1999, 16 (03): 201-207.  
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This paper summerized the studies on the halophytes in China which includes ( 1 ) the halophyte species; (2) the types of halophytes; (3) the vegetation types of halopytes; (4) halopy- tes failed to be recorded in Holoph of the world; (5) economic potential of halophytes. The further directions and key area of research on the Chinese halophytes were suggested at last.
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Discovery of Arabidopsis GRAS Family Genes in Response to Osmotic and Drought Stresses
Huajun Guo;Yuannian Jiao;Chao Di;Dongxia Yao;Gaihua Zhang;Xue Zheng;Lan Liu;Qunlian Zhang;Aiguang Guo*;Zhen Su*
Chinese Bulletin of Botany    2009, 44 (03): 290-299.   DOI: 10.3969/j.issn.1674-3466.2009.03.005
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The GRAS family is a class of plant specific transcription factors, playing essential roles in plant development and light signal transduction pathways. 33 GRAS family genes were identified in Arabidopsis genome. In this study, we found a group of GRAS family genes in response to osmotic and/or drought stresses through Arabidopsis GeneChip data mining. Meanwhile, we conducted co-expression analysis and gene ontology (GO) analysis and predicted that SCL13 was possibly involved in the response to osmotic stress. Our study will be helpful to elucidate some GRAS family genes related in signal transduction pathways during water stress, and be beneficial to crop molecular breeding in the future.

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Structure, Metabolic Pathway and Function of Sphingolipids in Plants
Runhua Liu;Wenbo Jiang;Diqiu Yu
Chinese Bulletin of Botany    2009, 44 (05): 619-628.   DOI: 10.3969/j.issn.1674-3466.2009.05.013
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Sphingolipids are ubiquitous membrane components in eukaryotic cells and in a few bacteria. Recent information indicated that sphingolipids are an impor tant class of messenger molecules involved in many significant signal trans duction pathways, regulating cell growth, differentiation, senescence, and apoptosis in mammals and yeast. However, the biological function of sphingolipids in plants has been explored only recently and remains elusive. Increasing investigation has shown that sphingolipids and their metabolites play an important role in plants. In this review, we describe the structure, metabolic pathway and biological function of sphingolipids.

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Rice Mutants and Functional Genomics
Longbiao Guo;Chengcai Chu;Qian Qian
Chinese Bulletin of Botany    2006, 23 (1): 1-13.  
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Mutants are important materials for research into functional genomics. This paper highlights the creation of rice mutants, variant mechanisms, classification and number of rice mutants, and cloned genes. Up to now, 1 698 mutants and genes and 43 cloned mutant genes have been identified.The prospects of rice mutants are discussed.
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Cited: CSCD(37)
The progresses of Studies on Abscisic Acid Receptors
WU Zhong-Yi;CHEN Jia and ZHU Mei-Jun
Chinese Bulletin of Botany    1998, 15 (04): 36-40.  
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Abscisic acid (ABA) participates in the control of diverse physioogical process. Although the molecular mechanism of ABA action is unknown, ABA receptor(s) have not yet been identified, high affinity of ABA binding proteins in some plants, either membrane-bound or soluble, have been described. The sense of ABA lies in the outside or inside of plasma membrane, there are some evidences with the techniques of microinjeetion. Considerable progress has been recently made in the characterization of more downstream elements of the ABA regulatory network, especially in the control of stomatal aperture by ABA. The details of the underlying signalling pathways start to emerge.
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Cited: CSCD(7)