Chinese Bulletin of Botany ›› 2020, Vol. 55 ›› Issue (1): 76-82.DOI: 10.11983/CBB19208

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Protocols for Analyzing Plant Phospho-proteins

Dan Zhu,Hanwei Cao,Yuan Li,Dongtao Ren()   

  1. State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China
  • Received:2019-10-24 Accepted:2019-12-31 Online:2020-01-01 Published:2020-01-03
  • Contact: Dongtao Ren

Abstract:

Protein phosphorylation is one of the important protein posttranslational modifications that is involved in the regulation of most cellular processes in plants. Protein kinases catalyze the phosphorylation by transferring the phosphate group in ATP to the substrate proteins. The phosphate is usually covalently linked to the hydroxyl group of specific amino acid residues in the substrates by an ester bond. The mostly studied phosphorylation sites are serine, threonine, and tyrosine residues. Here, we present protocols and related tips for the in vitro and in vivo protein phosphorylation assays.

Key words: protein phosphorylation, protein kinase, plant