Chinese Bulletin of Botany ›› 2020, Vol. 55 ›› Issue (1): 69-75.DOI: 10.11983/CBB19229

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Luciferase Complementation Assay for Detecting Protein Interactions

Yan Zhao(),Jianmin Zhou   

  1. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
  • Received:2019-11-24 Accepted:2019-12-02 Online:2020-01-01 Published:2019-12-20
  • Contact: Yan Zhao

Abstract: Protein-Protein interactions play important roles in various eukaryotic biological processes. Compared to other techniques measuring protein-protein interactions in plants, the Luciferase Complementation Assay (LCA), based on Agrobacterium-mediated transient expression in Nicotiana benthamiana, is a simple, sensitive, reliable, highly quantitative and low background method that can be easily scaled up for high-throughput interactome studies. Here, we describe a protocol that includes two alternative data collection methods to qualitative and quantitative analyse luminescence or luminous intensity to detect protein-protein interactions in plant cells.

Key words: luciferase complementation assay, protein-protein interactions, tobacco transient expression