%A Dan Zhu,Hanwei Cao,Yuan Li,Dongtao Ren %T Protocols for Analyzing Plant Phospho-proteins %0 Journal Article %D 2020 %J Chinese Bulletin of Botany %R 10.11983/CBB19208 %P 76-82 %V 55 %N 1 %U {https://www.chinbullbotany.com/CN/abstract/article_60381.shtml} %8 2020-01-01 %X

Protein phosphorylation is one of the important protein posttranslational modifications that is involved in the regulation of most cellular processes in plants. Protein kinases catalyze the phosphorylation by transferring the phosphate group in ATP to the substrate proteins. The phosphate is usually covalently linked to the hydroxyl group of specific amino acid residues in the substrates by an ester bond. The mostly studied phosphorylation sites are serine, threonine, and tyrosine residues. Here, we present protocols and related tips for the in vitro and in vivo protein phosphorylation assays.