Chinese Bulletin of Botany ›› 2021, Vol. 56 ›› Issue (4): 443-450.DOI: 10.11983/CBB20195

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Callus Induction and Somatic Embryogenesis in Anther Culture of Paeonia lactiflora

Yanmin Li, Hui Jiang, Zhenzhu Fu, Jing Zhang, Xin Yuan, Huijuan Wang, Jie Gao, Xiaoyu Dong, Limin Wang, Hechen Zhang*()   

  1. Horticultural Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
  • Received:2020-12-01 Accepted:2021-05-27 Online:2021-07-01 Published:2021-06-30
  • Contact: Hechen Zhang

Abstract: The anthers of Paeonia lactiflora cv. ‘Fenyunu’ were used as explants to study the effects of different concentrations of 2,4-D on callus induction, somatic embryogenesis and plant regeneration. The cell composition of callus and the development process of somatic embryos were observed with cytohistological method, and the ploidy of regenerated plants was identified using root tip squash method. The results showed that the suitable medium for callus induction of P. lactiflora anther was MS+1 mg·L-12,4-D+1 mg·L-1NAA+0.1 mg·L-1KT+30 g·L-1sucrose+6.5 g·L-1agar, and the callus induction rate was 14.7%. The callus was transferred to somatic embryo induction medium and underwent stages of spherical embryo, heart-shaped embryo, torpedo embryo and cotyledon embryo, and the somatic embryo induction rate was 52.1%. Genuine leaves germinated in seedling medium and complete plants were obtained, and the seedling rate was 47.1%. Haploid and diploid plants were identified using root tip squash method. The study preliminarily established a culture system to implement plant regeneration through somatic embryogenesis, which also provided reference protocol for anther culture of other varieties of Paeonia. Regenerated plants are important materials for genetic research and haploid breeding of P. lactiflora.

Key words: Paeonia lactiflora, anther culture, callus, somatic embryos, regenerated plants