Chinese Bulletin of Botany ›› 2024, Vol. 59 ›› Issue (3): 433-440.DOI: 10.11983/CBB23094

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Establishment of a Regeneration System for Lunaria annua

Hao Zeng1,2, Peifang Li1,2, Zhihui Guo1,2, Chunlin Liu1,3, Ying Ruan1,2,*()   

  1. 1Key Laboratory of Crop Epigenetic Regulation and Development, Hunan Agricultural University, Changsha 410128, China
    2College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China
    3College of Agronomy, Hunan Agricultural University, Changsha 410128, China
  • Received:2023-07-18 Accepted:2023-12-19 Online:2024-05-01 Published:2023-12-29
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Abstract: In order to establish an in vitro regeneration system for Lunaria annua, its true leaves were used as explants to study the effects of sterilization conditions, combinations and concentrations of plant growth regulator on the induction of callus and on the differentiation of adventitious buds and roots; The effect of rooting methods on the growth of seedlings and young plants was further explored. The results showed that the best disinfection treatment for leaf explants was a combination of 75% alcohol for 45 seconds and 0.1% HgCl2 solution for 6 minutes. The most suitable medium for induction of callus and differentiation of adventitious buds was MS+0.5 mg∙L-1 6-BA+2.0 mg∙L-1 2,4-D. The induction rate of callus reached 93.37% and the differentiation rate of adventitious buds reached 84.08%. The best rooting medium was MS+0.1 mg∙L-1 NAA, and the time from inoculating leaf explants to obtaining regenerated plants was about 90 days. In this study, a stable regeneration system was established, which laid a foundation for the development and utilization of L. annua resources and digging for functional genes.

Key words: Lunaria annua, callus, tissue culture, regeneration system