Abstract: In order to establish an in vitro regeneration system for Lunaria annua L., its real leaves were used as explants to study the effects of sterilization conditions, phytohormone combinations and concentrations on the induction of callus, adventitious shoots and adventitious roots differentiation; the effects of rooting methods on the growth of seedlings and young plants were further explored. The results showed that the best disinfection treatment for leaf explants was using a combination of 75% alcohol for 45 seconds and 0.1% HgCl₂ solution for 6 minutes. The most suitable medium for induction of real leaf callus and differentiation of adventitious shoots was MS + 0.5mg•L^-1 6-BA + 2.0mg•L^-1 2,4-D. The induction rate of callus reached 93.37% and the differentiation rate of adventitious shoots reached 84.08%. The best rooting medium was MS + 0.1mg•L^-1 NAA, and the time from inoculating leaf explants to obtaining regenerated plants was about 90 days. In this study, a stable regeneration system was established for the first time, which laid a foundation for the full development and utilization of Lunaria annua L. resources and the excavation of functional genes.

Key words: callus,  Lunaria annua,  regeneration system,  tissue culture