Chinese Bulletin of Botany ›› 2022, Vol. 57 ›› Issue (2): 217-226.DOI: 10.11983/CBB21200

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Establishment of Callus Induction and Regeneration System of Anemone silvestris

Jinchun Lu1, Lina Cao1, Guanjie Tong2, Xinying Wang1, Liying Zhang1, Xin Yu1, Huifang Li1, Yanhui Li1,*()   

  1. 1College of Landscape Architecture and Tourism, Hebei Agricultural University, Baoding 071000, China
    2College of Economics and Management, Hebei Agricultural University, Baoding 071000, China
  • Received:2021-11-18 Accepted:2022-01-04 Online:2022-03-01 Published:2022-03-24
  • Contact: Yanhui Li

Abstract:

In order to establish the tissue culture and regeneration system of wild Anemone silvestris, we investigated effects of different concentrations of plant growth regulators on callus induction, adventitious bud differentiation, proliferation and rooting of different explants including epicotyl, hypocotyl, leaf and petiole. The results showed that adventitious buds could be induced in all four explants, among them the epicotyl was the best. The best medium for hypocotyl induction was 1/2MS+2.0 mg∙L-1 6-BA+0.1 mg∙L-1 NAA with the highest induction rate of 86.67%; The optimal medium for proliferation was as follow 1/2MS+1.0 mg∙L-1 6-BA+0.05 mg∙L-1 NAA, and the proliferation coefficient was 3.67; The optimal rooting medium was 1/2MS+0.3 mg∙L-1 IBA with the rooting rate of 100%; In the medium of peat:vermiculite = 2:1 (v/v), the survival rate of tissue culture seedling was at 98.33%. This study effectively solved the shortage of germplasm resources of A. silvestris in garden and medicinal production, and provided technical support for the realization of factory seedling cultivation.

Key words: Anemone silvestris, callus, plant regeneration, tissue culture