Chinese Bulletin of Botany ›› 2023, Vol. 58 ›› Issue (3): 440-448.DOI: 10.11983/CBB22221

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Establishment of Highly Efficient Tissue Culture and Agrobacterium-mediated Callus Infection Systems for Hordeum brevisubulatum

Yefei Liu1,2, Haixia Zhao2, Xiping Jiang2, Rui Qiu1,2, Xinyue Zhou1, Yan Zhao1(), Chunxiang Fu2()   

  1. 1Key Laboratory of Grassland Resources of Ministry of Education/Key Laboratory of Forage Cultivation, Processing and Eficient Utilization of Ministry of Agriculture and Rural Areas/College of Grassland, Resources And Environment of Inner Mongolia Agricultural University, Hohhot 010018, China
    2Qingdao New Energy Shandong Laboratory, Shandong Key Laboratory of Energy Biogenetic Resources, Shandong Energy Research Institute, Key Laboratory of Biofuels, Chinese Academy of Sciences, Qingdao Institute of Bioenergy and Process, Chinese Academy of Sciences Qingdao 266101, China
  • Received:2022-09-13 Accepted:2022-11-15 Online:2023-05-01 Published:2023-05-17
  • Contact: *E-mail:;

Abstract: Hordeum brevisubulatum is a perennial grass belonging to the genus Hordeum in the Poaceae with strong cold and salt-alkali tolerance, which is an excellent germplasm resource for mining stress resistance genes. However, there are few reports on the genetic transformation system in H. brevisubulatum. In this study, the mature embryos in wild barley (Mengnong 1 hybrid) seeds were employed as explants to induce high quality embryogenic calli. A highly efficient tissue culture system was established for Mengnong 1 hybrid wild barley with approximate 70% of differentiation rate and the propagation coefficient was 35. Furthermore, Agrobacterium tumefaciens strain EHA105 was used to infect the wild barley callus line YZ101, and the infection efficiency was approximate 30% with optimized infection conditions. This study will facilitate gene function deciphering and developing novel germplasm in H. brevisubulatum through molecular design breeding.

Key words: Hordeum brevisubulatum, tissue culture, mature embryo, callus line, Agrobacterium infection