Chinese Bulletin of Botany ›› 2019, Vol. 54 ›› Issue (3): 378-384.DOI: 10.11983/CBB18181

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Tissue Culture and Rapid Propagation of Ardisia gigantifolia

Fengluan Tang,Jian Zhao,Zhiguo Zhao,Ke Xia,Shuo Qiu()   

  1. Guangxi Institute of Botany, Chinese Academy of Sciences and Guangxi Zhuangzu Autonomous Region, Guilin 541006, China
  • Received:2018-08-22 Accepted:2018-12-29 Online:2019-07-01 Published:2019-11-24
  • Contact: Shuo Qiu

Abstract: In this research, to establish a tissue culture and rapid propagation system of Ardisia gigantifolia, tender stem segments was selected as explants, and axillary buds were used to study proliferation. Culture medium of MS+1.0 mg·L -16-BA+0.2 mg·L -1 NAA and MS+0.5 mg·L -1 ZT had a significant effect on axillary bud induction and subculture of previous generations; the induction rates were 89.3% and 85.7%, respectively. The best culture medium for the multiplication was MS+0.5 mg·L -1 6-BA+0.1 mg·L -1 ZT+0.1 mg·L -1 NAA, and the propagation coefficient was 4.3. The optimal medium for rooting was 1/2MS+1.5 mg·L -1 IAA+1.0 mg·L -1NAA; the rooting rate reached 92.3%. The roots developed well and plantlets grew vigorously. Plantlets were transplanted to garden soil:peat soil:perlite=3:1:1 (v/v/v), and 82% rooted plants survived. The study established a fast propagation system for A. gigantifolia that could be applied to factory production.

Key words: Ardisia gigantifolia, axillary bud, tissue culture