Chinese Bulletin of Botany ›› 2019, Vol. 54 ›› Issue (6): 773-778.DOI: 10.11983/CBB18256
• TECHNIQUES AND METHODS • Previous Articles Next Articles
Wenting Zhang1,Yanhong He1,Ning Shu1,Jingjing Xing1,Baojun Liu1,Manzhu Bao1,Guofeng Liu2,*(
)
HomeReceived:2018-11-29
Accepted:2019-03-05
Online:2019-11-01
Published:2020-07-09
Contact:
Guofeng Liu
Wenting Zhang,Yanhong He,Ning Shu,Jingjing Xing,Baojun Liu,Manzhu Bao,Guofeng Liu. Plant Regeneration and Rapid Propagation System of Lilium bakerianum var. aureum[J]. Chinese Bulletin of Botany, 2019, 54(6): 773-778.
| Treatments | Explants | 6-BA (mg∙L-1) | NAA (mg∙L-1) | No. of explants | Survival rate (%) | No. of induced buds | No. of induced callus |
|---|---|---|---|---|---|---|---|
| 1 | Bulbs | 0 | 0 | 8 | 100 | 0 | 0 |
| 2 | Bulbs | 0.5 | 0.5 | 15 | 80.0 | 3 | 11 |
| 3 | Scale | 0 | 0 | 30 | 10.0 | 3 | 0 |
| 4 | Scale | 0.5 | 0.1 | 10 | 30.0 | 3 | 0 |
| 5 | Scale | 0.5 | 0.5 | 30 | 0 | 0 | 0 |
| 6 | Scale | 2.0 | 0.5 | 30 | 23.3 | 2 | 7 |
| 7 | Scale | 1.5 | 0.5 | 10 | 20.0 | 2 | 0 |
| 8 | Callus | 0 | 0 | 13 | 23.1 | 2 | 13 (browning) |
| 9 | Callus | 0.5 | 0.1 | 30 | 83.3 | 53 | 30 (swelled obviously) |
| 10 | Callus | 0.5 | 0.5 | 24 | 62.5 | 46 | 24 (callus proliferation) |
| 11 | Callus | 2.0 | 0.5 | 15 | 80.0 | 14 | 15 (bulbs differentiation) |
| 12 | Callus | 1.0 | 0.1 | 22 | 81.8 | 71 | 22 (budding obviously) |
| 13 | Petiole | 0.5 | 0.1 | 118 | 5.9 | 0 | 7 |
| 14 | Petiole | 0.5 | 0.5 | 60 | 6.7 | 1 | 3 |
| 15 | Petiole | 2.0 | 0.5 | 60 | 0 | 0 | 0 |
| 16 | Petiole | 1.0 | 0.1 | 90 | 4.4 | 9 | 0 |
| 17 | Leaf | 0.5 | 0.1 | 60 | 0 | 0 | 0 |
| 18 | Leaf | 0.5 | 0.5 | 60 | 0 | 0 | 0 |
| 19 | Leaf | 2.0 | 0.5 | 60 | 0 | 0 | 0 |
Table 1 Comparison of regeneration capabilities of different explants from Lilium bakerianum var. aureum
| Treatments | Explants | 6-BA (mg∙L-1) | NAA (mg∙L-1) | No. of explants | Survival rate (%) | No. of induced buds | No. of induced callus |
|---|---|---|---|---|---|---|---|
| 1 | Bulbs | 0 | 0 | 8 | 100 | 0 | 0 |
| 2 | Bulbs | 0.5 | 0.5 | 15 | 80.0 | 3 | 11 |
| 3 | Scale | 0 | 0 | 30 | 10.0 | 3 | 0 |
| 4 | Scale | 0.5 | 0.1 | 10 | 30.0 | 3 | 0 |
| 5 | Scale | 0.5 | 0.5 | 30 | 0 | 0 | 0 |
| 6 | Scale | 2.0 | 0.5 | 30 | 23.3 | 2 | 7 |
| 7 | Scale | 1.5 | 0.5 | 10 | 20.0 | 2 | 0 |
| 8 | Callus | 0 | 0 | 13 | 23.1 | 2 | 13 (browning) |
| 9 | Callus | 0.5 | 0.1 | 30 | 83.3 | 53 | 30 (swelled obviously) |
| 10 | Callus | 0.5 | 0.5 | 24 | 62.5 | 46 | 24 (callus proliferation) |
| 11 | Callus | 2.0 | 0.5 | 15 | 80.0 | 14 | 15 (bulbs differentiation) |
| 12 | Callus | 1.0 | 0.1 | 22 | 81.8 | 71 | 22 (budding obviously) |
| 13 | Petiole | 0.5 | 0.1 | 118 | 5.9 | 0 | 7 |
| 14 | Petiole | 0.5 | 0.5 | 60 | 6.7 | 1 | 3 |
| 15 | Petiole | 2.0 | 0.5 | 60 | 0 | 0 | 0 |
| 16 | Petiole | 1.0 | 0.1 | 90 | 4.4 | 9 | 0 |
| 17 | Leaf | 0.5 | 0.1 | 60 | 0 | 0 | 0 |
| 18 | Leaf | 0.5 | 0.5 | 60 | 0 | 0 | 0 |
| 19 | Leaf | 2.0 | 0.5 | 60 | 0 | 0 | 0 |
| Treatments | 6-BA (mg∙L-1) | NAA (mg∙L-1) | GA3 (mg∙L-1) | Induction ratio (%) |
|---|---|---|---|---|
| 1 | 0.5 | 0 | 0 | 23.3±21.00 b |
| 2 | 0.5 | 0.1 | 0 | 33.3±6.00 b |
| 3 | 1.0 | 0.1 | 0 | 50.00±10.00 a |
| 4 | 1.0 | 0.1 | 1.0 | 0.00±0.00 d |
| 5 | 1.5 | 0.5 | 0 | 6.70±6.00 cd |
Table 2 Effects of plant growth regulators on the induction of callus and adventitious buds of Lilium bakerianum var. aureum
| Treatments | 6-BA (mg∙L-1) | NAA (mg∙L-1) | GA3 (mg∙L-1) | Induction ratio (%) |
|---|---|---|---|---|
| 1 | 0.5 | 0 | 0 | 23.3±21.00 b |
| 2 | 0.5 | 0.1 | 0 | 33.3±6.00 b |
| 3 | 1.0 | 0.1 | 0 | 50.00±10.00 a |
| 4 | 1.0 | 0.1 | 1.0 | 0.00±0.00 d |
| 5 | 1.5 | 0.5 | 0 | 6.70±6.00 cd |
| Treatments | 6-BA (mg∙L-1) | NAA (mg∙L-1) | Proliferation multiple | Growth state |
|---|---|---|---|---|
| 1 | 0.5 | 0.1 | 3.87±0.24 a | Strong |
| 2 | 0.5 | 0.5 | 1.87±0.18 b | Sparse, slightly yellow |
| 3 | 1.0 | 0.1 | 1.06±0.17 c | Sparse, yellow |
| 4 | 1.0 | 0.5 | 1.23±0.09 c | Sparse, yellow |
| 5 | 2.0 | 0.5 | 0.93±0.24 c | Sparse, yellow |
Table 3 Effects of plant growth regulators on the multiplication of adventitious buds of Lilium bakerianum var. aureum
| Treatments | 6-BA (mg∙L-1) | NAA (mg∙L-1) | Proliferation multiple | Growth state |
|---|---|---|---|---|
| 1 | 0.5 | 0.1 | 3.87±0.24 a | Strong |
| 2 | 0.5 | 0.5 | 1.87±0.18 b | Sparse, slightly yellow |
| 3 | 1.0 | 0.1 | 1.06±0.17 c | Sparse, yellow |
| 4 | 1.0 | 0.5 | 1.23±0.09 c | Sparse, yellow |
| 5 | 2.0 | 0.5 | 0.93±0.24 c | Sparse, yellow |
| Treatments | 6-BA (mg∙L-1) | NAA (mg∙L-1) | No. of induced bulbs | Growth state |
|---|---|---|---|---|
| 1 | 0 | 0.5 | 1.73±0.23 d | Sparse leaves, thick roots |
| 2 | 0 | 1.0 | 4.70±0.46 a | Sparse leaves, thick roots |
| 3 | 0 | 2.0 | 2.47±0.00 cd | Thick leaves, thick roots |
| 4 | 0.2 | 0.5 | 2.80±1.00 c | Yellow leaves, rootless |
| 5 | 0.2 | 1.0 | 2.03±0.32 d | Yellow leaves, rootless |
| 6 | 0.2 | 2.0 | 3.50±0.42 b | Strong leaves, thick roots |
Table 4 Effects of plant growth regulators on the induction of bulb from adventitious buds of Lilium bakerianum var. aureum
| Treatments | 6-BA (mg∙L-1) | NAA (mg∙L-1) | No. of induced bulbs | Growth state |
|---|---|---|---|---|
| 1 | 0 | 0.5 | 1.73±0.23 d | Sparse leaves, thick roots |
| 2 | 0 | 1.0 | 4.70±0.46 a | Sparse leaves, thick roots |
| 3 | 0 | 2.0 | 2.47±0.00 cd | Thick leaves, thick roots |
| 4 | 0.2 | 0.5 | 2.80±1.00 c | Yellow leaves, rootless |
| 5 | 0.2 | 1.0 | 2.03±0.32 d | Yellow leaves, rootless |
| 6 | 0.2 | 2.0 | 3.50±0.42 b | Strong leaves, thick roots |
| Treatments | MS | Sucrose (g∙L-1) | NAA (mg∙L-1) | IBA (mg∙L-1) | Swell times | Growth state |
|---|---|---|---|---|---|---|
| 1 | 0.5 | 120 | 0.3 | 0.1 | 2.63±0.35 b | Withered leaves, thick roots |
| 2 | 0.5 | 90 | 0.3 | 0.1 | 1.94±0.41 c | Sparse leaves, thick roots |
| 3 | 0.5 | 60 | 0.3 | 0.1 | 0.57±0.29 d | Rootless |
| 4 | 0.5 | 120 | 0.3 | 0 | 1.57±0.06 c | Curly leaves, black roots |
| 5 | 0.5 | 90 | 0.3 | 0 | 1.58±0.14 c | Thick leaves, thin scales |
| 6 | 0.5 | 60 | 0.3 | 0 | 1.00±0.25 d | Thick leaves, thin roots |
| 7 | 0.5 | 60 | 0.01 | 0 | 2.78±0.19 ab | Thick leaves, thick roots |
| 8 | 0.5 | 60 | 0.1 | 0 | 2.67±0.33 ab | Sparse leaves, thin roots |
Table 5 Effects of plant growth regulators on the bulb expansion and rooting of Lilium bakerianum var. aureum
| Treatments | MS | Sucrose (g∙L-1) | NAA (mg∙L-1) | IBA (mg∙L-1) | Swell times | Growth state |
|---|---|---|---|---|---|---|
| 1 | 0.5 | 120 | 0.3 | 0.1 | 2.63±0.35 b | Withered leaves, thick roots |
| 2 | 0.5 | 90 | 0.3 | 0.1 | 1.94±0.41 c | Sparse leaves, thick roots |
| 3 | 0.5 | 60 | 0.3 | 0.1 | 0.57±0.29 d | Rootless |
| 4 | 0.5 | 120 | 0.3 | 0 | 1.57±0.06 c | Curly leaves, black roots |
| 5 | 0.5 | 90 | 0.3 | 0 | 1.58±0.14 c | Thick leaves, thin scales |
| 6 | 0.5 | 60 | 0.3 | 0 | 1.00±0.25 d | Thick leaves, thin roots |
| 7 | 0.5 | 60 | 0.01 | 0 | 2.78±0.19 ab | Thick leaves, thick roots |
| 8 | 0.5 | 60 | 0.1 | 0 | 2.67±0.33 ab | Sparse leaves, thin roots |
Figure 1 Establishment of plantlet regeneration from scales of Lilium bakerianum var. aureum (A), (B) Induction of new bud from scales; (C) Differentiation of new bud from callus; (D) The multiplication culture of adventitious buds; (E) The forming of bulb from adventitious bud; (F) Regenerated plantlets; (G) Plantlets rooting; (H), (I) Transplanting. Bars=1 cm
| [1] | 胡新颖, 杨迎东, 颜津宁, 颜范悦 ( 2014). 百合鳞片全基质包埋试验. 江苏农业科学 42(2), 147-149. |
| [2] | 黄宇翔, 陈华, 刘金燕 ( 2005). 东方百合鳞片扦插繁殖研究. 中国农学通报 21(10), 273-275. |
| [3] | 李黛, 谈锋 ( 2004). 诱导百合鳞片芽的影响因子研究. 种子 23(11), 18-20. |
| [4] | 刘木清, 秦龙, 伍春 ( 2016). 百合繁殖技术. 花木盆景(花卉园艺) ( 2), 29-31. |
| [5] | 罗凤霞, 徐桂华, 金丽丽, 孙晓梅, 朱明 ( 2000). 新铁炮百合微繁的研究. 沈阳农业大学学报 31, 254-257. |
| [6] | 宁云芬, 周厚高, 黄玉源, 叶向斌, 王凤兰, 黄子锋 ( 2002). 百合种球繁育的研究进展. 仲恺农业技术学院学报 15(2), 66-70. |
| [7] | 舒宁 ( 2015). 几种云南野生百合资源调查引种及离体繁殖体系研究. 硕士论文. 武汉: 华中农业大学. pp. 44-45. |
| [8] | 舒宁, 詹文静, 刘燕妮, 刘国锋 ( 2015). 大理百合的组织培养研究. 植物学研究 4, 8-15. |
| [9] | 王家福 (2006). 花卉组织培养与快繁技术. 北京: 中国林业出版社. pp. 258-266. |
| [10] | 吴青青, 窦云, 张朝君, 石乐娟 胡小京 ( 2015). 两个百合商业品种的组培快繁技术研究. 北方园艺 ( 12), 96-99. |
| [11] | 虞泓, 陆永武, 程治英 ( 2005). 大百合的离体快繁和鳞茎的诱导. 植物生理学通讯 41, 192. |
| [12] | 袁素霞, 李佳, 明军, 刘春, 徐雷锋, 袁迎迎 ( 2015). 百合未授粉子房离体培养胚胎形成及植株再生. 植物学报 50, 378-387. |
| [13] | 张建华, 庄天明, 陈银华 ( 2006). 百合无病毒苗快速繁殖技术. 上海交通大学学报(农业科学版) 24, 370-373. |
| [14] | 张旭红, 王頔, 梁振旭, 孙美玉, 张金政, 石雷 ( 2018). 欧洲百合愈伤组织诱导及植株再生体系的建立. 植物学报 53, 840-847. |
| [15] | 张云, 原雅玲, 刘青林 ( 2001). 百合品种改良与生物技术研究进展. 北京林业大学学报 23(6), 56-59. |
| [16] | 中国科学院中国植物志编辑委员会 ( 1980). 中国植物志. 北京: 科学出版社. pp. 137. |
| [17] | Arzate-Fernández AM, Nakazaki T, Okumoto Y, Tanisaka T ( 1997). Efficient callus induction and plant regeneration from filaments with anther in lily (Lilium longiflorum Thunb.). Plant Cell Rep 16, 836-840. |
| [18] | Ault JR, Siqueira SS ( 2008). Morphogenetic response of Lilium michiganense to four auxin-type plant growth regulators in vitro. HortScience 43, 1922-1924. |
| [19] | Bahr LR, Compton ME ( 2004). Competence for in vitro bulblet regeneration among eight Lilium genotypes. HortScience 39, 127-129. |
| [20] | Karalija E, Trbojević S, Parić A ( 2010). Somatic embryogenesis and in vitro plantlet regeneration of Lilium martagon L. var. cattaniae Vis. Biol Nyssana 1, 57-60. |
| [21] | Nhut DT, Van Le B, Fukai S, Tanaka M, Van KTT ( 2001). Effects of activated charcoal, explant size, explant position and sucrose concentration on plant and shoot regeneration of Lilium longiflorum via young stem culture. Plant Growth Regul 33, 59-65. |
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