Plant Regeneration and Rapid Propagation System of Lilium bakerianum var. aureum

doi:10.11983/CBB18256

Abstract

Abstract: We investigated the effects of different explants, kinds and concentrations of plant growth regulators on callus induction and plant regeneration from scales of Lilium bakerianum var. aureum at tissue culture. The medium MS+1.0 mg∙L ^-16-BA+0.1 mg∙L ^-1NAA+30 g∙L ^-1 sucrose was suitable for inducing adventitious buds and calli. The medium MS+0.5 mg∙L ^-16-BA+0.1 mg∙L ^-1NAA+30 g∙L ^-1 sucrose was suitable for adventitious bud multiplication. MS+1.0 mg∙L ^-1NAA+30 g∙L ^-1 sucrose was optimal for bulblet formation from adventitious buds, and the optimal medium for the growth of bulblets was 1/2MS+0.01 mg∙L ^-1NAA+60 g∙L ^-1 sucrose. We provide technical support for germplasm resource protection and rapid propagation of L. bakerianum var. aureum.

Key words: Lilium bakerianum var. aureum, tissue culture, plant regeneration, rapid propagation, calli

Wenting Zhang,Yanhong He,Ning Shu,Jingjing Xing,Baojun Liu,Manzhu Bao,Guofeng Liu. Plant Regeneration and Rapid Propagation System of Lilium bakerianum var. aureum[J]. Chinese Bulletin of Botany, 2019, 54(6): 773-778.

Add to citation manager EndNote|Ris|BibTeX

URL: https://www.chinbullbotany.com/EN/10.11983/CBB18256

https://www.chinbullbotany.com/EN/Y2019/V54/I6/773

Figures/Tables 6

References 21

[1]	胡新颖, 杨迎东, 颜津宁, 颜范悦 ( 2014). 百合鳞片全基质包埋试验. 江苏农业科学 42(2), 147-149.
[2]	黄宇翔, 陈华, 刘金燕 ( 2005). 东方百合鳞片扦插繁殖研究. 中国农学通报 21(10), 273-275.
[3]	李黛, 谈锋 ( 2004). 诱导百合鳞片芽的影响因子研究. 种子 23(11), 18-20.
[4]	刘木清, 秦龙, 伍春 ( 2016). 百合繁殖技术. 花木盆景(花卉园艺) ( 2), 29-31.
[5]	罗凤霞, 徐桂华, 金丽丽, 孙晓梅, 朱明 ( 2000). 新铁炮百合微繁的研究. 沈阳农业大学学报 31, 254-257.
[6]	宁云芬, 周厚高, 黄玉源, 叶向斌, 王凤兰, 黄子锋 ( 2002). 百合种球繁育的研究进展. 仲恺农业技术学院学报 15(2), 66-70.
[7]	舒宁 ( 2015). 几种云南野生百合资源调查引种及离体繁殖体系研究. 硕士论文. 武汉: 华中农业大学. pp. 44-45.
[8]	舒宁, 詹文静, 刘燕妮, 刘国锋 ( 2015). 大理百合的组织培养研究. 植物学研究 4, 8-15.
[9]	王家福 (2006). 花卉组织培养与快繁技术. 北京: 中国林业出版社. pp. 258-266.
[10]	吴青青, 窦云, 张朝君, 石乐娟胡小京 ( 2015). 两个百合商业品种的组培快繁技术研究. 北方园艺 ( 12), 96-99.
[11]	虞泓, 陆永武, 程治英 ( 2005). 大百合的离体快繁和鳞茎的诱导. 植物生理学通讯 41, 192.
[12]	袁素霞, 李佳, 明军, 刘春, 徐雷锋, 袁迎迎 ( 2015). 百合未授粉子房离体培养胚胎形成及植株再生. 植物学报 50, 378-387.
[13]	张建华, 庄天明, 陈银华 ( 2006). 百合无病毒苗快速繁殖技术. 上海交通大学学报(农业科学版) 24, 370-373.
[14]	张旭红, 王頔, 梁振旭, 孙美玉, 张金政, 石雷 ( 2018). 欧洲百合愈伤组织诱导及植株再生体系的建立. 植物学报 53, 840-847.
[15]	张云, 原雅玲, 刘青林 ( 2001). 百合品种改良与生物技术研究进展. 北京林业大学学报 23(6), 56-59.
[16]	中国科学院中国植物志编辑委员会 ( 1980). 中国植物志. 北京: 科学出版社. pp. 137.
[17]	Arzate-Fernández AM, Nakazaki T, Okumoto Y, Tanisaka T ( 1997). Efficient callus induction and plant regeneration from filaments with anther in lily (Lilium longiflorum Thunb.). Plant Cell Rep 16, 836-840.
[18]	Ault JR, Siqueira SS ( 2008). Morphogenetic response of Lilium michiganense to four auxin-type plant growth regulators in vitro. HortScience 43, 1922-1924.
[19]	Bahr LR, Compton ME ( 2004). Competence for in vitro bulblet regeneration among eight Lilium genotypes. HortScience 39, 127-129.
[20]	Karalija E, Trbojević S, Parić A ( 2010). Somatic embryogenesis and in vitro plantlet regeneration of Lilium martagon L. var. cattaniae Vis. Biol Nyssana 1, 57-60.
[21]	Nhut DT, Van Le B, Fukai S, Tanaka M, Van KTT ( 2001). Effects of activated charcoal, explant size, explant position and sucrose concentration on plant and shoot regeneration of Lilium longiflorum via young stem culture. Plant Growth Regul 33, 59-65.

Treatments	6-BA (mg∙L^-1)	NAA (mg∙L^-1)	GA₃(mg∙L^-1)	Induction ratio (%)
1	0.5	0	0	23.3±21.00 b
2	0.5	0.1	0	33.3±6.00 b
3	1.0	0.1	0	50.00±10.00 a
4	1.0	0.1	1.0	0.00±0.00 d
5	1.5	0.5	0	6.70±6.00 cd

Treatments	6-BA (mg∙L^-1)	NAA (mg∙L^-1)	GA₃(mg∙L^-1)	Induction ratio (%)
1	0.5	0	0	23.3±21.00 b
2	0.5	0.1	0	33.3±6.00 b
3	1.0	0.1	0	50.00±10.00 a
4	1.0	0.1	1.0	0.00±0.00 d
5	1.5	0.5	0	6.70±6.00 cd

Treatments	6-BA (mg∙L^-1)	NAA (mg∙L^-1)	Proliferation multiple	Growth state
1	0.5	0.1	3.87±0.24 a	Strong
2	0.5	0.5	1.87±0.18 b	Sparse, slightly yellow
3	1.0	0.1	1.06±0.17 c	Sparse, yellow
4	1.0	0.5	1.23±0.09 c	Sparse, yellow
5	2.0	0.5	0.93±0.24 c	Sparse, yellow

Treatments	6-BA (mg∙L^-1)	NAA (mg∙L^-1)	Proliferation multiple	Growth state
1	0.5	0.1	3.87±0.24 a	Strong
2	0.5	0.5	1.87±0.18 b	Sparse, slightly yellow
3	1.0	0.1	1.06±0.17 c	Sparse, yellow
4	1.0	0.5	1.23±0.09 c	Sparse, yellow
5	2.0	0.5	0.93±0.24 c	Sparse, yellow

Treatments	6-BA (mg∙L^-1)	NAA (mg∙L^-1)	No. of induced bulbs	Growth state
1	0	0.5	1.73±0.23 d	Sparse leaves, thick roots
2	0	1.0	4.70±0.46 a	Sparse leaves, thick roots
3	0	2.0	2.47±0.00 cd	Thick leaves, thick roots
4	0.2	0.5	2.80±1.00 c	Yellow leaves, rootless
5	0.2	1.0	2.03±0.32 d	Yellow leaves, rootless
6	0.2	2.0	3.50±0.42 b	Strong leaves, thick roots