Abstract: Utilizing the seed kernel of Xanthoceras sorbifolium Bunge as the experimental material, this study aims to identify the optimal culture medium for the induction of adventitious buds and to investigate the factors influencing the browning of the explants.The results showed that the best method for seed kernel disinfection was to use 75% ethanol for 30 seconds and 0.1% effective chlorine for 10 minutes, with a contamination rate of 29.33% and a mortality rate of 12%. The optimal medium formula for inducing adventitious buds is MS+2.5 mg · L-1 6-BA+1.0 mg · L-1 NAA+30 g · L-1 sucrose+6.8 g · L-1 agar+0.1 g · L-1 inositol, with an induction rate of 72.22%; Among various cytokinin plant growth regulators, the addition of 6-BA to the culture medium resulted in the lowest explant mortality rate of 12.50% and the highest induction rate of 73.61%. The optimal cultivation conditions were a light intensity of 1300lx and a temperature of 26°C, with the highest induction rate of 72.22%. Seeds harvested from July 1st to July 8th exhibit plump and firm endosperms, with white seed coats that can be easily separated. These characteristics render them the most suitable explants for mitigating browning during in vitro culture of Xanthoceras sorbifolium Bunge, and the highest induction rate of adventitious buds is 97.22%.

Key words: Xanthoceras sorbifolium, seed kernels, tissue culture, browning