Effect of Conservation Conditions on Restricting Conservation of Acer rubrum cv. ‘Somerset’

doi:10.11983/CBB18010

Abstract

Abstract: We examined the effect of conservation conditions on restricting the conservation of Acer rubrum L. cv. ‘Somerset’, including photoperiod, light intensity, temperature and diurnal temperature variation. In the 182-day conservation period, the in vitro materials were mainly for differentiation in the early period and vegetative growth in the later period, also gradually adapted to the low-temperature environment. The effect of temperature on the growth of in vitro materials reached extremely significant level (P<0.01); the effect of photoperiod and light intensity lasted for a long time, and their interaction reached significant level (P<0.05). Diurnal temperature variation had a significant effect on average number of shoots and rate of rooting. In all of the tests, 25°C, illumination 12 h, 62.50 μmol·m ^-2·s ^-1 and 25°C, illumination 12 h, 31.25 μmol·m ^-2·s ^-1 conditions were both able to restrict conservation. The selection criteria for the best conservation conditions was not unique, and the core was keeping the differentiation ability of the in vitro materials.

Key words: restricting conservation, in vitro conservation, tissue culture, growth rhythm

Yang Xian,Xin Dong,Xiaoman Xie,Dan Wu,Biao Han,Yan Wang. Effect of Conservation Conditions on Restricting Conservation of Acer rubrum cv. ‘Somerset’[J]. Chinese Bulletin of Botany, 2019, 54(1): 64-71.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB18010

https://www.chinbullbotany.com/EN/Y2019/V54/I1/64

Figures/Tables 6

References 17

1	兰伟, 陈发棣 ( 2010). 植物种质资源缓慢生长法保存研究进展. 阜阳师范学院学报(自然科学版) 27(2), 68-72. DOI URL
2	刘静波, 林士杰, 张忠辉, 谢朋, 蔡群, 张大伟, 勾天兵, 赵珊珊, 赵洪伟, 王志强 ( 2012). 槭属植物种质资源研究进展. 中国农学通报 28(25), 1-5. DOI URL
3	任桂萍, 王小菁, 朱根发 ( 2016). 不同光质的LED对蝴蝶兰组织培养增殖及生根的影响. 植物学报 51, 81-88. DOI URL
4	咸洋, 吴丹, 解孝满, 刘德深, 韩彪, 田莎莎, 刘丹 ( 2014). 一种红花槭的组培快繁方法. 中国专利, 201310493738.1. 2014-02-05.
5	徐呈祥 ( 2012). 提高植物抗寒性的机理研究进展. 生态学报 32, 7966-7980. DOI URL
6	徐刚标 ( 2000). 植物种质资源离体保存研究进展. 中南林学院学报 20(4), 81-87. DOI URL
7	燕丽萍, 李丽, 刘翠兰, 吴德军, 王因花, 任飞, 赵梁军 ( 2016). 绒毛白蜡体胚诱导和植株再生. 植物学报 51, 807-816. DOI URL
8	杨博, 曹秀婷, 王政, 何松林 ( 2012). 昼夜温差对非洲菊试管苗生长的影响. 西北林学院学报 27(2), 88-92. DOI URL
9	张红岩, 吴小芹, 朱丽华, 谈家金 ( 2010). 抗病赤松组培丛生芽低温保存条件研究. 南京林业大学学报(自然科学版) 34(5), 7-11. DOI URL
10	郑卫杰, 郭子霞, 王政, 何松林 ( 2011). 昼夜温差对文心兰试管苗生长的影响. 西北林学院学报 26(4), 137-141.
11	周逊, 向长萍 ( 2008). 植物种质资源缓慢生长离体保存研究进展. 中国蔬菜 ( 11), 39-42.
12	Ahmad N, Abbasi BH, Fazal H, Khan MA, Afridi MS ( 2014). Effect of reverse photoperiod on in vitro regene- ration and piperine production in Piper nigrum L.C R Biologies 337, 19-28.
13	Jouve L, Franck T, Gaspars T, Cattivelli L, Hausman JF ( 2000). Poplar acclimation to cold during in vitro conservation at low non-freezing temperature: metabolic and proteic changes.J Plant Physiol 157, 117-123. DOI URL
14	Kozai T, Watanabe K, Jeong BR ( 1995). Stem elongation and growth of Solanum tuberosum L. in vitro in response to photosynthetic photon flux, photoperiod and difference in photoperiod and dark period temperatures.Sci Hortic 64, 1-9. DOI URL
15	Salcedo-Morales G, Rosas-Romero G, Nabor-Correa N, Bermúdez-Torres K, López-Laredo AR, Trejo-Tapia G ( 2009). Propagation and conservation of Castilleja tenu- iflora Benth.Polibotánica ( 28), 119-137.
16	Sarasan V, Cripps R, Ramsay MM, Atherton C, Mcmi- chen M, Prendergast G, Rowntree JK ( 2006). Conservation in vitro of threatened plants—progress in the past decade.In Vitro Cell Dev Biol Plant 42, 206-214. DOI URL
17	Silveira DG, Souza FVD, Pelacani CR, da Silva Souza A, da Silva Ledo CA, de Santana JRF ( 2009). Micropropagation and in vitro conservation of Neoglaziovia variegata (Arr. Cam.) Mez, a fiber producing bromeliad from Brazil. Braz Arch Biol Technol 52, 923-932.

	Conservation days	Significance testing of P and LI			Significance testing of Tm	Significance testing of DTV and LI
	Conservation days	P	LI	P×LI	Tm	DTV	LI	DTV×LI
Differentiation rate of explants	7	0^**	0.018^*	0.002^**	0^**	0.05	0.84	0.589
	14	0^**	0.004^**	0.036^*	0.005^**	0.914	0.009^**	0.115
	56	0.002^**	0.075	0.001^**	0^**	0.771	0.283	0.302
	182	0.297	0.907	0.01^*	0^**	0.322	0.86	0.564
Average number of shoots	7	0^**	0^**	0^**	0^**	0^**	0.695	0.248
	14	0^**	0.003^**	0.002^**	0.009^**	0.013^*	0.772	0.008^**
	28	0^**	0.236	0^**	0^**	0.8	0.488	0.479
	49	0.017^*	0.248	0.033^*	0^**	0.003^**	0.244	0.563
	98	0.129	0.113	0.237	0.001^**	0.036^*	0.209	0.574
	147	0.167	0.011^*	0.193	0.006^**	0.383	0.217	0.992
	182	0.057	0.001^**	0.008^**	0.131	0.016^*	0.03^*	0.063
Average height	14	0.589	0.516	0.751	0.081	0.071	0.641	0.680
	35	0.113	0.322	0.942	0^**	0.845	0.077	0.744
	91	0.021^*	0^**	0.004^**	0.002^**	0.464	0^**	0.670
	126	0.733	0.002^**	0.039^*	0.004^**	0.636	0.016^*	0.422
	154	0.207	0.291	0.093	0.003^**	0.646	0.549	0.501
	182	0.187	0.294	0.182	0^**	0.009^**	0.034^*	0.185
Rate of rooting	21	0.004^**	0.041^*	0.213	0.037^*	0.541	0.025^*	0.504
	28	0^**	0^**	0.002^**	0.001^**	0.225	0.004^**	0.658
	35	0^**	0^**	0.012^*	0^**	0.409	0.008^**	0.885
	42	0.885	0.35	0^**	0^**	0.896	0.003^**	0.336
	49	0^**	0.002^**	0^**	0^**	0.018^*	0.006^**	0.415
	56	0^**	0.001^**	0^**	0^**	0.005^**	0.036^*	0.905
	63	0^**	0.329	0.004^**	0^**	0.01^*	0.49	0.432
	70	0^**	0.156	0.001^**	0^**	0.054	0.448	0.423
	182	0.015^*	0.284	0.007^**	0^**	0.09	0.713	0.901

	Conservation days	Significance testing of P and LI			Significance testing of Tm	Significance testing of DTV and LI
	Conservation days	P	LI	P×LI	Tm	DTV	LI	DTV×LI
Differentiation rate of explants	7	0^**	0.018^*	0.002^**	0^**	0.05	0.84	0.589
	14	0^**	0.004^**	0.036^*	0.005^**	0.914	0.009^**	0.115
	56	0.002^**	0.075	0.001^**	0^**	0.771	0.283	0.302
	182	0.297	0.907	0.01^*	0^**	0.322	0.86	0.564
Average number of shoots	7	0^**	0^**	0^**	0^**	0^**	0.695	0.248
	14	0^**	0.003^**	0.002^**	0.009^**	0.013^*	0.772	0.008^**
	28	0^**	0.236	0^**	0^**	0.8	0.488	0.479
	49	0.017^*	0.248	0.033^*	0^**	0.003^**	0.244	0.563
	98	0.129	0.113	0.237	0.001^**	0.036^*	0.209	0.574
	147	0.167	0.011^*	0.193	0.006^**	0.383	0.217	0.992
	182	0.057	0.001^**	0.008^**	0.131	0.016^*	0.03^*	0.063
Average height	14	0.589	0.516	0.751	0.081	0.071	0.641	0.680
	35	0.113	0.322	0.942	0^**	0.845	0.077	0.744
	91	0.021^*	0^**	0.004^**	0.002^**	0.464	0^**	0.670
	126	0.733	0.002^**	0.039^*	0.004^**	0.636	0.016^*	0.422
	154	0.207	0.291	0.093	0.003^**	0.646	0.549	0.501
	182	0.187	0.294	0.182	0^**	0.009^**	0.034^*	0.185
Rate of rooting	21	0.004^**	0.041^*	0.213	0.037^*	0.541	0.025^*	0.504
	28	0^**	0^**	0.002^**	0.001^**	0.225	0.004^**	0.658
	35	0^**	0^**	0.012^*	0^**	0.409	0.008^**	0.885
	42	0.885	0.35	0^**	0^**	0.896	0.003^**	0.336
	49	0^**	0.002^**	0^**	0^**	0.018^*	0.006^**	0.415
	56	0^**	0.001^**	0^**	0^**	0.005^**	0.036^*	0.905
	63	0^**	0.329	0.004^**	0^**	0.01^*	0.49	0.432
	70	0^**	0.156	0.001^**	0^**	0.054	0.448	0.423
	182	0.015^*	0.284	0.007^**	0^**	0.09	0.713	0.901

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