Effect of Conservation Conditions on Restricting Conservation of Acer rubrum cv. ‘Somerset’

doi:10.11983/CBB18010

Abstract

Abstract: We examined the effect of conservation conditions on restricting the conservation of Acer rubrum L. cv. ‘Somerset’, including photoperiod, light intensity, temperature and diurnal temperature variation. In the 182-day conservation period, the in vitro materials were mainly for differentiation in the early period and vegetative growth in the later period, also gradually adapted to the low-temperature environment. The effect of temperature on the growth of in vitro materials reached extremely significant level (P<0.01); the effect of photoperiod and light intensity lasted for a long time, and their interaction reached significant level (P<0.05). Diurnal temperature variation had a significant effect on average number of shoots and rate of rooting. In all of the tests, 25°C, illumination 12 h, 62.50 μmol·m ^-2·s ^-1 and 25°C, illumination 12 h, 31.25 μmol·m ^-2·s ^-1 conditions were both able to restrict conservation. The selection criteria for the best conservation conditions was not unique, and the core was keeping the differentiation ability of the in vitro materials.

Key words: restricting conservation, in vitro conservation, tissue culture, growth rhythm

Yang Xian,Xin Dong,Xiaoman Xie,Dan Wu,Biao Han,Yan Wang. Effect of Conservation Conditions on Restricting Conservation of Acer rubrum cv. ‘Somerset’[J]. Chinese Bulletin of Botany, 2019, 54(1): 64-71.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB18010

https://www.chinbullbotany.com/EN/Y2019/V54/I1/64

Figures/Tables 6

References 17

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	Conservation days	Significance testing of P and LI			Significance testing of Tm	Significance testing of DTV and LI
	Conservation days	P	LI	P×LI	Tm	DTV	LI	DTV×LI
Differentiation rate of explants	7	0^**	0.018^*	0.002^**	0^**	0.05	0.84	0.589
	14	0^**	0.004^**	0.036^*	0.005^**	0.914	0.009^**	0.115
	56	0.002^**	0.075	0.001^**	0^**	0.771	0.283	0.302
	182	0.297	0.907	0.01^*	0^**	0.322	0.86	0.564
Average number of shoots	7	0^**	0^**	0^**	0^**	0^**	0.695	0.248
	14	0^**	0.003^**	0.002^**	0.009^**	0.013^*	0.772	0.008^**
	28	0^**	0.236	0^**	0^**	0.8	0.488	0.479
	49	0.017^*	0.248	0.033^*	0^**	0.003^**	0.244	0.563
	98	0.129	0.113	0.237	0.001^**	0.036^*	0.209	0.574
	147	0.167	0.011^*	0.193	0.006^**	0.383	0.217	0.992
	182	0.057	0.001^**	0.008^**	0.131	0.016^*	0.03^*	0.063
Average height	14	0.589	0.516	0.751	0.081	0.071	0.641	0.680
	35	0.113	0.322	0.942	0^**	0.845	0.077	0.744
	91	0.021^*	0^**	0.004^**	0.002^**	0.464	0^**	0.670
	126	0.733	0.002^**	0.039^*	0.004^**	0.636	0.016^*	0.422
	154	0.207	0.291	0.093	0.003^**	0.646	0.549	0.501
	182	0.187	0.294	0.182	0^**	0.009^**	0.034^*	0.185
Rate of rooting	21	0.004^**	0.041^*	0.213	0.037^*	0.541	0.025^*	0.504
	28	0^**	0^**	0.002^**	0.001^**	0.225	0.004^**	0.658
	35	0^**	0^**	0.012^*	0^**	0.409	0.008^**	0.885
	42	0.885	0.35	0^**	0^**	0.896	0.003^**	0.336
	49	0^**	0.002^**	0^**	0^**	0.018^*	0.006^**	0.415
	56	0^**	0.001^**	0^**	0^**	0.005^**	0.036^*	0.905
	63	0^**	0.329	0.004^**	0^**	0.01^*	0.49	0.432
	70	0^**	0.156	0.001^**	0^**	0.054	0.448	0.423
	182	0.015^*	0.284	0.007^**	0^**	0.09	0.713	0.901

	Conservation days	Significance testing of P and LI			Significance testing of Tm	Significance testing of DTV and LI
	Conservation days	P	LI	P×LI	Tm	DTV	LI	DTV×LI
Differentiation rate of explants	7	0^**	0.018^*	0.002^**	0^**	0.05	0.84	0.589
	14	0^**	0.004^**	0.036^*	0.005^**	0.914	0.009^**	0.115
	56	0.002^**	0.075	0.001^**	0^**	0.771	0.283	0.302
	182	0.297	0.907	0.01^*	0^**	0.322	0.86	0.564
Average number of shoots	7	0^**	0^**	0^**	0^**	0^**	0.695	0.248
	14	0^**	0.003^**	0.002^**	0.009^**	0.013^*	0.772	0.008^**
	28	0^**	0.236	0^**	0^**	0.8	0.488	0.479
	49	0.017^*	0.248	0.033^*	0^**	0.003^**	0.244	0.563
	98	0.129	0.113	0.237	0.001^**	0.036^*	0.209	0.574
	147	0.167	0.011^*	0.193	0.006^**	0.383	0.217	0.992
	182	0.057	0.001^**	0.008^**	0.131	0.016^*	0.03^*	0.063
Average height	14	0.589	0.516	0.751	0.081	0.071	0.641	0.680
	35	0.113	0.322	0.942	0^**	0.845	0.077	0.744
	91	0.021^*	0^**	0.004^**	0.002^**	0.464	0^**	0.670
	126	0.733	0.002^**	0.039^*	0.004^**	0.636	0.016^*	0.422
	154	0.207	0.291	0.093	0.003^**	0.646	0.549	0.501
	182	0.187	0.294	0.182	0^**	0.009^**	0.034^*	0.185
Rate of rooting	21	0.004^**	0.041^*	0.213	0.037^*	0.541	0.025^*	0.504
	28	0^**	0^**	0.002^**	0.001^**	0.225	0.004^**	0.658
	35	0^**	0^**	0.012^*	0^**	0.409	0.008^**	0.885
	42	0.885	0.35	0^**	0^**	0.896	0.003^**	0.336
	49	0^**	0.002^**	0^**	0^**	0.018^*	0.006^**	0.415
	56	0^**	0.001^**	0^**	0^**	0.005^**	0.036^*	0.905
	63	0^**	0.329	0.004^**	0^**	0.01^*	0.49	0.432
	70	0^**	0.156	0.001^**	0^**	0.054	0.448	0.423
	182	0.015^*	0.284	0.007^**	0^**	0.09	0.713	0.901

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