Chinese Bulletin of Botany ›› 2023, Vol. 58 ›› Issue (6): 926-934.DOI: 10.11983/CBB23060

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Establishment of In Vitro Regeneration System of Citrus australasica

Chungang Xie1,2, Zhe Liu1, Shusheng Zhang3, Haitao Hu1,*()   

  1. 1College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China
    2Wildlife Protection and Management Station, Jinhua Municipal Bureau of Planning and Natural Resources, Jinhua 321052, China
    3The Management Center of Wuyanling National Natural Reserve in Zhejiang, Taishun 325500, China
  • Received:2023-05-12 Accepted:2023-09-19 Online:2023-11-01 Published:2023-11-27
  • Contact: * E-mail:

Abstract: In order to establish the regeneration system of Citrus australasica, the effects of different plant growth regulators combinations, medium types and dark culture time on callus induction and plant regeneration of C. australasica were studied using stem segments as explants. The results showed that the best medium for adventitious bud induction was 1/2MS+4.0 mg∙L-1 ZT+30.0 g∙L-1 sucrose. Dark culture for 14 days and then light culture had the best promoting effect. The induction rate of callus and adventitious bud was 100%, and the average number of adventitious bud regeneration per explants was 4.83. The optimal rooting medium was 1/2MS+0.5 mg∙L-1 NAA, and the regenerated plants with the 94.43% rooting rate were obtained, and the average number of roots was 3.9. In the mixture of grass carbon:perlite: vermiculite=2:1:1 (v/v/v), tissue culture seedlings had the best growth, and the survival rate was more than 90%. This study established in vitro regeneration system of C. australasica, which laid the foundation for the genetic improvement and rapid propagation of C. australasica fine varieties.

Key words: Citrus australasica, stem segment, tissue culture, in vitro regeneration