Chinese Bulletin of Botany ›› 2019, Vol. 54 ›› Issue (5): 634-641.DOI: 10.11983/CBB18193

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

The Effect of Different Hormones on Explant Browning and Callus Browning in Cyclocarya paliurus

Ying Feng1,*(),Lianwen Qian1,Qingliang Lin2   

  1. 1. School of Resource and Environmental Science, Quanzhou Normal University, Quanzhou 362000, China
    2. Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2018-09-11 Accepted:2019-02-19 Online:2019-09-01 Published:2020-03-10
  • Contact: Ying Feng


To determine the effect of different hormones on explant browning and callus browning in Cyclocarya paliurus, leaf browning was controlled on medium with different kinds and content of hormones (6-BA, GA3, NAA) by a single-factor completely random test. Callus browning was controlled on the medium with different content of 6-BA+NAA and different basic medium by a double- and single-factor completely random test, respectively. The rate of leaf browning reached 0, and the rate of callus induction was up to 100% on the improved MS medium with 1.0 mg∙L -1 6-BA and 1.0 mg∙L -1 GA3 and 0.3 mg∙L -1 NAA. The rate of callus browning reached 0, and the callus propagation was up to 4.80 times on the improved MS medium with 0.5 mg∙L -1 6-BA and 0.2 mg∙L -1NAA. The callus was yellow with green or yellow, granular, small and compact, hard and dry. This system effectively solved the problem of leaf browning and callus browning and provided a simple and effective way for tissue culture and a solid foundation for the regeneration system from leaf of C. paliurus.

Key words: browning, Cyclocarya paliurus, callus, induction, propagation