Chinese Bulletin of Botany ›› 2019, Vol. 54 ›› Issue (5): 634-641.DOI: 10.11983/CBB18193
• TECHNIQUES AND METHODS • Previous Articles Next Articles
Ying Feng1,*(
),Lianwen Qian1,Qingliang Lin2
HomeReceived:2018-09-11
Accepted:2019-02-19
Online:2019-09-01
Published:2020-03-10
Contact:
Ying Feng
Ying Feng,Lianwen Qian,Qingliang Lin. The Effect of Different Hormones on Explant Browning and Callus Browning in Cyclocarya paliurus[J]. Chinese Bulletin of Botany, 2019, 54(5): 634-641.
| Treatment | 6-BA content (mg·L-1) | GA3 content (mg·L-1) | NAA content (mg·L-1) |
|---|---|---|---|
| 6-BA1 | 1.0 | 1.0 | 0.3 |
| 6-BA2 | 2.0 | 1.0 | 0.3 |
| 6-BA3 | 3.0 | 1.0 | 0.3 |
| GA31 | 1.0 | 0.0 | 0.3 |
| GA32 | 1.0 | 0.5 | 0.3 |
| GA33 | 1.0 | 1.0 | 0.3 |
| GA34 | 1.0 | 1.5 | 0.3 |
| NAA1 | 1.0 | 1.0 | 0.0 |
| NAA2 | 1.0 | 1.0 | 0.1 |
| NAA3 | 1.0 | 1.0 | 0.3 |
| NAA4 | 1.0 | 1.0 | 0.5 |
Table 1 6-BA+GA3+NAA hormone combination
| Treatment | 6-BA content (mg·L-1) | GA3 content (mg·L-1) | NAA content (mg·L-1) |
|---|---|---|---|
| 6-BA1 | 1.0 | 1.0 | 0.3 |
| 6-BA2 | 2.0 | 1.0 | 0.3 |
| 6-BA3 | 3.0 | 1.0 | 0.3 |
| GA31 | 1.0 | 0.0 | 0.3 |
| GA32 | 1.0 | 0.5 | 0.3 |
| GA33 | 1.0 | 1.0 | 0.3 |
| GA34 | 1.0 | 1.5 | 0.3 |
| NAA1 | 1.0 | 1.0 | 0.0 |
| NAA2 | 1.0 | 1.0 | 0.1 |
| NAA3 | 1.0 | 1.0 | 0.3 |
| NAA4 | 1.0 | 1.0 | 0.5 |
Figure 1 The type of callus induced from leaves of Cyclocarya paliurus (A) Callus of the type I; (B) Callus of the type II; (C) Callus of the type III. Bars=1 cm
| Medium | The rate of callus induction (%) | The rate of leaf browning (%) | Callus | |
|---|---|---|---|---|
| Type | Growth | |||
| 1.0 mg·L-1 6-BA+1.0 mg·L-1 GA3+0.3 mg·L-1 NAA | 100.00±0.00 a | 0.00±0.00 a | I, II | I (++), II (+) |
| 2.0 mg·L-1 6-BA+1.0 mg·L-1 GA3+0.3 mg·L-1 NAA | 95.24±8.25 a | 4.76±8.25 b | I, II | I (+++), II (++) |
| 3.0 mg·L-1 6-BA+1.0 mg·L-1 GA3+0.3 mg·L-1 NAA | 66.67±18.04 b | 33.33±18.04 b | I, III | I (++), III (+) |
Table 2 The effect of 6-BA on leaf browning in Cyclocarya paliurus (means±SD)
| Medium | The rate of callus induction (%) | The rate of leaf browning (%) | Callus | |
|---|---|---|---|---|
| Type | Growth | |||
| 1.0 mg·L-1 6-BA+1.0 mg·L-1 GA3+0.3 mg·L-1 NAA | 100.00±0.00 a | 0.00±0.00 a | I, II | I (++), II (+) |
| 2.0 mg·L-1 6-BA+1.0 mg·L-1 GA3+0.3 mg·L-1 NAA | 95.24±8.25 a | 4.76±8.25 b | I, II | I (+++), II (++) |
| 3.0 mg·L-1 6-BA+1.0 mg·L-1 GA3+0.3 mg·L-1 NAA | 66.67±18.04 b | 33.33±18.04 b | I, III | I (++), III (+) |
| Medium | The rate of callus induction (%) | The rate of leaf browning (%) | Callus | |
|---|---|---|---|---|
| Type | Growth | |||
| 1.0 mg·L-1 6-BA+0.3 mg·L-1 NAA | 48.96±4.77 c | 51.04±4.77 a | I, III | I (++), III (+) |
| 0.5 mg·L-1 GA3+1.0 mg·L-1 6-BA+0.3 mg·L-1 NAA | 86.67±10.41 b | 13.33±10.41 b | I, II, III | I (++), II (+), III (-) |
| 1.0 mg·L-1 GA3+1.0 mg·L-1 6-BA+0.3 mg·L-1 NAA | 100.00±0.00 a | 0.00±0.00 c | I, II | I (++), II (+) |
| 1.5 mg·L-1 GA3+1.0 mg·L-1 6-BA+0.3 mg·L-1 NAA | 86.11±2.78 b | 13.89±2.78 b | I | I (++) |
Table 3 The effect of GA3 on leaf browning in Cyclocarya paliurus (means±SD)
| Medium | The rate of callus induction (%) | The rate of leaf browning (%) | Callus | |
|---|---|---|---|---|
| Type | Growth | |||
| 1.0 mg·L-1 6-BA+0.3 mg·L-1 NAA | 48.96±4.77 c | 51.04±4.77 a | I, III | I (++), III (+) |
| 0.5 mg·L-1 GA3+1.0 mg·L-1 6-BA+0.3 mg·L-1 NAA | 86.67±10.41 b | 13.33±10.41 b | I, II, III | I (++), II (+), III (-) |
| 1.0 mg·L-1 GA3+1.0 mg·L-1 6-BA+0.3 mg·L-1 NAA | 100.00±0.00 a | 0.00±0.00 c | I, II | I (++), II (+) |
| 1.5 mg·L-1 GA3+1.0 mg·L-1 6-BA+0.3 mg·L-1 NAA | 86.11±2.78 b | 13.89±2.78 b | I | I (++) |
Figure 2 The effect of GA3 on callus induction of leaves in Cyclocarya paliurus (A) Callus in the induction medium without GA3; (B) Callus cultured for 15 days in the induction medium with GA3; (C) Callus cultured for 30 days in the induction medium with GA3. Bars=1 cm
| Medium | The rate of callus induction (%) | The rate of leaf browning (%) | Callus | |
|---|---|---|---|---|
| Type | Growth | |||
| 1.0 mg·L-1 GA3+1.0 mg·L-1 6-BA | 23.15±11.25 c | 76.85±11.25 a | I | I (-) |
| 0.1 mg·L-1 NAA+1.0 mg·L-1 GA3+1.0 mg·L-1 6-BA | 71.39±7.01 b | 18.61±7.01 b | I, II | I (++), II (+) |
| 0.3 mg·L-1 NAA+1.0 mg·L-1 GA3+1.0 mg·L-1 6-BA | 100.00±0.00 a | 0.00±0.00 c | I, II | I (++), II (+) |
| 0.5 mg·L-1 NAA+1.0 mg·L-1 GA3+1.0 mg·L-1 6-BA | 96.99±0.20 a | 3.11±0.20 c | I, II | I (+), II (+) |
Table 4 The effect of NAA on leaf browning in Cyclocarya paliurus (means±SD)
| Medium | The rate of callus induction (%) | The rate of leaf browning (%) | Callus | |
|---|---|---|---|---|
| Type | Growth | |||
| 1.0 mg·L-1 GA3+1.0 mg·L-1 6-BA | 23.15±11.25 c | 76.85±11.25 a | I | I (-) |
| 0.1 mg·L-1 NAA+1.0 mg·L-1 GA3+1.0 mg·L-1 6-BA | 71.39±7.01 b | 18.61±7.01 b | I, II | I (++), II (+) |
| 0.3 mg·L-1 NAA+1.0 mg·L-1 GA3+1.0 mg·L-1 6-BA | 100.00±0.00 a | 0.00±0.00 c | I, II | I (++), II (+) |
| 0.5 mg·L-1 NAA+1.0 mg·L-1 GA3+1.0 mg·L-1 6-BA | 96.99±0.20 a | 3.11±0.20 c | I, II | I (+), II (+) |
Figure 3 The type of callus propagation in Cyclocarya paliurus (A), (B) The callus of type I; (C), (D) The callus of type II; (E), (F) The callus of type III. Bars=1 cm
| Plant growth regulator (mg·L-1) | The time of callus propagation (time) | The rate of callus browning (%) | Callus | ||
|---|---|---|---|---|---|
| 6-BA | NAA | Type | Growth | ||
| 0.2 | 0.05 | 1.25 de | 0 e | II | II (+++) |
| 0.5 | 0.05 | 3.87 b | 0 e | I, II | I (+++), II (++) |
| 0.8 | 0.05 | 3.51 b | 0 e | I, II | I (++), II (+++) |
| 1.0 | 0.05 | 0.69 e | 0 e | I, III | I (++), III (+) |
| 0.2 | 0.2 | 0.75 e | 0 e | I, II, III | I (+), II (+), III (+) |
| 0.5 | 0.2 | 4.80 a | 0 e | I, II | I (++), II (+++) |
| 0.8 | 0.2 | 3.55 b | 0 e | I, II, III | I (++), II (+++), III (++++) |
| 1.0 | 0.2 | 1.30 de | 40.00 a | I, II | I (++), II (+) |
| 0.2 | 0.4 | 0.97 de | 33.33 ab | I, III | I (+), III (+) |
| 0.5 | 0.4 | 1.65 d | 28.33 bc | II, III | II (+), III (+) |
| 0.8 | 0.4 | 2.57 c | 20.00 d | II, III | II (++), III (+) |
| 1.0 | 0.4 | 1.75 d | 21.67 cd | II, III | II (+), III (+) |
Table 5 The effect of 6-BA+NAA on callus browning in Cyclocarya paliurus
| Plant growth regulator (mg·L-1) | The time of callus propagation (time) | The rate of callus browning (%) | Callus | ||
|---|---|---|---|---|---|
| 6-BA | NAA | Type | Growth | ||
| 0.2 | 0.05 | 1.25 de | 0 e | II | II (+++) |
| 0.5 | 0.05 | 3.87 b | 0 e | I, II | I (+++), II (++) |
| 0.8 | 0.05 | 3.51 b | 0 e | I, II | I (++), II (+++) |
| 1.0 | 0.05 | 0.69 e | 0 e | I, III | I (++), III (+) |
| 0.2 | 0.2 | 0.75 e | 0 e | I, II, III | I (+), II (+), III (+) |
| 0.5 | 0.2 | 4.80 a | 0 e | I, II | I (++), II (+++) |
| 0.8 | 0.2 | 3.55 b | 0 e | I, II, III | I (++), II (+++), III (++++) |
| 1.0 | 0.2 | 1.30 de | 40.00 a | I, II | I (++), II (+) |
| 0.2 | 0.4 | 0.97 de | 33.33 ab | I, III | I (+), III (+) |
| 0.5 | 0.4 | 1.65 d | 28.33 bc | II, III | II (+), III (+) |
| 0.8 | 0.4 | 2.57 c | 20.00 d | II, III | II (++), III (+) |
| 1.0 | 0.4 | 1.75 d | 21.67 cd | II, III | II (+), III (+) |
| Plant growth regulator | The time of callus propagation (time) | The rate of callus browning (%) |
|---|---|---|
| 0.2 mg·L-1 6-BA | 0.98±0.33 b | 11.11±17.64 b |
| 0.5 mg·L-1 6-BA | 3.44±1.47 a | 9.44±15.09 b |
| 0.8 mg·L-1 6-BA | 3.21±0.67 a | 6.67±10.00 b |
| 1.0 mg·L-1 6-BA | 1.25±0.55 b | 20.56±17.40 a |
| 0.05 mg·L-1 NAA | 2.33±1.49 a | 0.00±0.00 c |
| 0.2 mg·L-1 NAA | 2.59±1.75 a | 10.00±18.09 b |
| 0.4 mg·L-1 NAA | 1.73±0.74 b | 25.83±8.75 a |
Table 6 The effect of 6-BA and NAA on callus browning in Cyclocarya paliurus, respectively (means±SD)
| Plant growth regulator | The time of callus propagation (time) | The rate of callus browning (%) |
|---|---|---|
| 0.2 mg·L-1 6-BA | 0.98±0.33 b | 11.11±17.64 b |
| 0.5 mg·L-1 6-BA | 3.44±1.47 a | 9.44±15.09 b |
| 0.8 mg·L-1 6-BA | 3.21±0.67 a | 6.67±10.00 b |
| 1.0 mg·L-1 6-BA | 1.25±0.55 b | 20.56±17.40 a |
| 0.05 mg·L-1 NAA | 2.33±1.49 a | 0.00±0.00 c |
| 0.2 mg·L-1 NAA | 2.59±1.75 a | 10.00±18.09 b |
| 0.4 mg·L-1 NAA | 1.73±0.74 b | 25.83±8.75 a |
Figure 4 The effect of basic medium on callus browning (A) and callus propagation (B) in Cyclocarya paliurus (means±SD) Different lowercase letters indicate significant differences at P<0.05.
Figure 5 Callus of Cyclocarya paliurus growth in the different basic medium (A) Callus growth in the improved MS basic medium; (B), (C) Callus growth in the improved MS1 basic medium; (D), (E) Callus growth in the improved MS2 basic medium; (F) Callus growth in the improved MS3 basic medium. Bars=1 cm
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