The Effect of Different Hormones on Explant Browning and Callus Browning in Cyclocarya paliurus

doi:10.11983/CBB18193

Abstract

Abstract:

To determine the effect of different hormones on explant browning and callus browning in Cyclocarya paliurus, leaf browning was controlled on medium with different kinds and content of hormones (6-BA, GA₃, NAA) by a single-factor completely random test. Callus browning was controlled on the medium with different content of 6-BA+NAA and different basic medium by a double- and single-factor completely random test, respectively. The rate of leaf browning reached 0, and the rate of callus induction was up to 100% on the improved MS medium with 1.0 mg∙L ^-1 6-BA and 1.0 mg∙L ^-1 GA₃ and 0.3 mg∙L ^-1 NAA. The rate of callus browning reached 0, and the callus propagation was up to 4.80 times on the improved MS medium with 0.5 mg∙L ^-1 6-BA and 0.2 mg∙L ^-1NAA. The callus was yellow with green or yellow, granular, small and compact, hard and dry. This system effectively solved the problem of leaf browning and callus browning and provided a simple and effective way for tissue culture and a solid foundation for the regeneration system from leaf of C. paliurus.

Key words: browning, Cyclocarya paliurus, callus, induction, propagation

Ying Feng,Lianwen Qian,Qingliang Lin. The Effect of Different Hormones on Explant Browning and Callus Browning in Cyclocarya paliurus[J]. Chinese Bulletin of Botany, 2019, 54(5): 634-641.

Figures/Tables 11

References 25

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Medium	The rate of callus induction (%)	The rate of leaf browning (%)	Callus
Medium	The rate of callus induction (%)	The rate of leaf browning (%)	Type	Growth
1.0 mg·L^-1 6-BA+1.0 mg·L^-1 GA₃+0.3 mg·L^-1 NAA	100.00±0.00 a	0.00±0.00 a	I, II	I (++), II (+)
2.0 mg·L^-1 6-BA+1.0 mg·L^-1 GA₃+0.3 mg·L^-1 NAA	95.24±8.25 a	4.76±8.25 b	I, II	I (+++), II (++)
3.0 mg·L^-1 6-BA+1.0 mg·L^-1 GA₃+0.3 mg·L^-1 NAA	66.67±18.04 b	33.33±18.04 b	I, III	I (++), III (+)

Medium	The rate of callus induction (%)	The rate of leaf browning (%)	Callus
Medium	The rate of callus induction (%)	The rate of leaf browning (%)	Type	Growth
1.0 mg·L^-1 6-BA+1.0 mg·L^-1 GA₃+0.3 mg·L^-1 NAA	100.00±0.00 a	0.00±0.00 a	I, II	I (++), II (+)
2.0 mg·L^-1 6-BA+1.0 mg·L^-1 GA₃+0.3 mg·L^-1 NAA	95.24±8.25 a	4.76±8.25 b	I, II	I (+++), II (++)
3.0 mg·L^-1 6-BA+1.0 mg·L^-1 GA₃+0.3 mg·L^-1 NAA	66.67±18.04 b	33.33±18.04 b	I, III	I (++), III (+)

Medium	The rate of callus induction (%)	The rate of leaf browning (%)	Callus
Medium	The rate of callus induction (%)	The rate of leaf browning (%)	Type	Growth
1.0 mg·L^-16-BA+0.3 mg·L^-1 NAA	48.96±4.77 c	51.04±4.77 a	I, III	I (++), III (+)
0.5 mg·L^-1GA₃+1.0 mg·L^-16-BA+0.3 mg·L^-1 NAA	86.67±10.41 b	13.33±10.41 b	I, II, III	I (++), II (+), III (-)
1.0 mg·L^-1GA₃+1.0 mg·L^-16-BA+0.3 mg·L^-1 NAA	100.00±0.00 a	0.00±0.00 c	I, II	I (++), II (+)
1.5 mg·L^-1GA₃+1.0 mg·L^-16-BA+0.3 mg·L^-1 NAA	86.11±2.78 b	13.89±2.78 b	I	I (++)

Medium	The rate of callus induction (%)	The rate of leaf browning (%)	Callus
Medium	The rate of callus induction (%)	The rate of leaf browning (%)	Type	Growth
1.0 mg·L^-16-BA+0.3 mg·L^-1 NAA	48.96±4.77 c	51.04±4.77 a	I, III	I (++), III (+)
0.5 mg·L^-1GA₃+1.0 mg·L^-16-BA+0.3 mg·L^-1 NAA	86.67±10.41 b	13.33±10.41 b	I, II, III	I (++), II (+), III (-)
1.0 mg·L^-1GA₃+1.0 mg·L^-16-BA+0.3 mg·L^-1 NAA	100.00±0.00 a	0.00±0.00 c	I, II	I (++), II (+)
1.5 mg·L^-1GA₃+1.0 mg·L^-16-BA+0.3 mg·L^-1 NAA	86.11±2.78 b	13.89±2.78 b	I	I (++)

Medium	The rate of callus induction (%)	The rate of leaf browning (%)	Callus
Medium	The rate of callus induction (%)	The rate of leaf browning (%)	Type	Growth
1.0 mg·L^-1 GA₃+1.0 mg·L^-16-BA	23.15±11.25 c	76.85±11.25 a	I	I (-)
0.1 mg·L^-1NAA+1.0 mg·L^-1 GA₃+1.0 mg·L^-16-BA	71.39±7.01 b	18.61±7.01 b	I, II	I (++), II (+)
0.3 mg·L^-1NAA+1.0 mg·L^-1 GA₃+1.0 mg·L^-16-BA	100.00±0.00 a	0.00±0.00 c	I, II	I (++), II (+)
0.5 mg·L^-1NAA+1.0 mg·L^-1 GA₃+1.0 mg·L^-16-BA	96.99±0.20 a	3.11±0.20 c	I, II	I (+), II (+)