An Efficient System for Regenerating Adventitious Buds in Stem Segments of Tea Plants

doi:10.11983/CBB22184

Abstract

Abstract: Tea plant is an important economic crop with high heterozygosity and variation. There are few reports on the in vitro regeneration system with high efficiency. In this study, the stem segment of a tea cultivar Shuchazao was used as the initial explants to identify the factors which could efficiently influence the regeneration of adventitious buds. The results showed that MS+2 mg∙L^-1 6-BA was the best formula for normal buds induction, and the induction rate was 84.44%. The expansion rate of the absorption chassis was 80%, which was beneficial to the subsequent induction of adventitious buds. MS+2 mg∙L^-1 6-BA+0.2 mg∙L^-1 NAA+0.1 mg∙L^-1 KT+1 mg∙L^-1 proline was the suitable formula for the induction of adventitious buds, and the induction rate was 88.89%. The average number of shoots was 7.8. 1/2MS+3 mg∙L^-1 IBA was the suitable formula for adventitious root induction, and the rooting rate was 85.56%. The regenerated plants were subjected to molecular verification by RAPD and ISSR techniques. The results showed that no significant variation was found in the two consecutive generations of regenerated plants in vitro.

Key words: Camellia sinensis, in vitro regeneration, adventitious bud induction, genetic stability

Lulu Ren, Youze Zhang, Kelin Huang, Xiaochun Wan, Zhaoliang Zhang, Mulan Zhu, Chaoling Wei. An Efficient System for Regenerating Adventitious Buds in Stem Segments of Tea Plants[J]. Chinese Bulletin of Botany, 2023, 58(2): 308-315.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB22184

https://www.chinbullbotany.com/EN/Y2023/V58/I2/308

Figures/Tables 10

References 21

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Basic medium	State of growth
WPM	The bottom callus starts quickly and grows well, the petiole turns red, a small number of normal buds
MS	The bottom callus is thickened, the fixed buds are robust, a small number of normal buds, the foliage is emerald green, and the whole culture is viable
N6	The bottom callus starts quickly, the petiole is reddish, and the leaves are thin
DCR	There is less callus at the bottom and the leaves are thin and yellowish

Basic medium	State of growth
WPM	The bottom callus starts quickly and grows well, the petiole turns red, a small number of normal buds
MS	The bottom callus is thickened, the fixed buds are robust, a small number of normal buds, the foliage is emerald green, and the whole culture is viable
N6	The bottom callus starts quickly, the petiole is reddish, and the leaves are thin
DCR	There is less callus at the bottom and the leaves are thin and yellowish

6-BA (mg?L^-1)	Bud induction rate (%)	Bottom expansion rate (%)	State of growth
0	5.56±1.92 d	1.11±1.92 d	The growth is weak and the base becomes brown
1	51.11±5.09 c	61.11±3.84 c	The growth is better, with fewer buds and calli form at the base
2	77.78±5.09 a	80.00±3.33 b	The growth is strong, with fixed buds at the axillary buds and callus at the base
3	84.44±1.92 a	78.89±6.93 b	The growth is better, with fixed buds at the axillary buds and more callus at the base
4	58.89±3.84 b	88.89±5.09 a	The growth is weak, the axillary buds are yellow, and the chassis is expanded and partially lobed

6-BA (mg?L^-1)	Bud induction rate (%)	Bottom expansion rate (%)	State of growth
0	5.56±1.92 d	1.11±1.92 d	The growth is weak and the base becomes brown
1	51.11±5.09 c	61.11±3.84 c	The growth is better, with fewer buds and calli form at the base
2	77.78±5.09 a	80.00±3.33 b	The growth is strong, with fixed buds at the axillary buds and callus at the base
3	84.44±1.92 a	78.89±6.93 b	The growth is better, with fixed buds at the axillary buds and more callus at the base
4	58.89±3.84 b	88.89±5.09 a	The growth is weak, the axillary buds are yellow, and the chassis is expanded and partially lobed

Number	6-BA (mg?L^-1)	NAA (mg?L^-1)	Adventitious bud elongation rate (%)
1	0.2	0.02	24.44±6.94 c
2	0.4	0.04	30.00±3.33 c
3	0.6	0.06	58.89±1.92 b
4	0.8	0.08	70.00±8.82 a
5	1	0.1	51.11±5.09 b