%A Ying Feng,Lianwen Qian,Qingliang Lin %T The Effect of Different Hormones on Explant Browning and Callus Browning in Cyclocarya paliurus %0 Journal Article %D 2019 %J Chinese Bulletin of Botany %R 10.11983/CBB18193 %P 634-641 %V 54 %N 5 %U {https://www.chinbullbotany.com/CN/abstract/article_59681.shtml} %8 2019-09-01 %X

To determine the effect of different hormones on explant browning and callus browning in Cyclocarya paliurus, leaf browning was controlled on medium with different kinds and content of hormones (6-BA, GA3, NAA) by a single-factor completely random test. Callus browning was controlled on the medium with different content of 6-BA+NAA and different basic medium by a double- and single-factor completely random test, respectively. The rate of leaf browning reached 0, and the rate of callus induction was up to 100% on the improved MS medium with 1.0 mg∙L -1 6-BA and 1.0 mg∙L -1 GA3 and 0.3 mg∙L -1 NAA. The rate of callus browning reached 0, and the callus propagation was up to 4.80 times on the improved MS medium with 0.5 mg∙L -1 6-BA and 0.2 mg∙L -1NAA. The callus was yellow with green or yellow, granular, small and compact, hard and dry. This system effectively solved the problem of leaf browning and callus browning and provided a simple and effective way for tissue culture and a solid foundation for the regeneration system from leaf of C. paliurus.