Chinese Bulletin of Botany ›› 2023, Vol. 58 ›› Issue (3): 449-460.DOI: 10.11983/CBB22135

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Establishment of Regeneration System of Chrysanthemum indicum in Pingtan with Various Ligulate Floret Form

Minling Liao1, Ya Pu1, Xiaoyun Wu1, Chaofeng Ma1, Wenkui Wang2, Silan Dai1()   

  1. 1Beijing Advanced Innovation Center for Tree Breeding by Molecular Design; Laboratory of Urban and Rural Ecological Environment, Beijing Key Laboratory of Flower Germplasm Innovation and Molecular Breeding, National Flower Engineering Technology Research Center, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China
    2Fuzhou Planning Design and Research Institute Group Co. Ltd., Fuzhou 350108, China
  • Received:2022-06-28 Accepted:2022-09-26 Online:2023-05-01 Published:2023-05-17
  • Contact: *E-mail: silandai@sina.com

Abstract: Asteraceae plants have high ornamental value due to their rich petal variation in ray florets. Chrysanthemum indicum is a wild species and closely related to C. × morifolium. Ray florets mutants of C. indicum in natural populations, such as flat, spoon and tubular types, are excellent material for studying petal shapes. However, there is currently a lack of research on its regeneration system. In this experiment, considerable ray floret mutants of C. indicum were found on Pingtan Island, Fujian province, including the plants with flat, spoon and tubular types of ray florets coexisting in the same capitulum, in which leaves and transverse thin cell layers (tTCLs) were used as explants to establish a regeneration system. The results showed that the best medium for callus induction and adventitious bud differentiation of tTCLs was MS+1.0 mg∙L-1 6-BA+0.5 mg∙L-1 NAA, and the induction rate was 100% on the 14th day. The average differentiation time was 25 days, and the differentiation rate reached 82% on the 40th day. The best rooting medium was 1/2MS+0.5 mg∙L-1 NAA, and the rooting time was 10 days. All of the rooted and regenerated seedlings were survived after transplanting and kept the mix type in capitulum. An efficient regeneration system for C. indicum in Pingtan with various ray floret forms was established in this experiment. The results not only laid the foundation for the establishment of a transformation system but also provided technology for future research on the morphological variation in ray florets.

Key words: Chrysanthemum indicum, plant growth regulator, regeneration system, stains with various ligulate floret form, transverse thin cell layer