Chinese Bulletin of Botany ›› 2020, Vol. 55 ›› Issue (5): 605-612.DOI: 10.11983/CBB20030

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Optimization of the Regeneration System from Somatic Embryogenesis in Larix olgensis

Jianfei Liu1, Yan Liu1, Kejian Liu2, Yang Chi3, Zhifa Huo3, Yonghong Huo3, Xiangling You1,*()   

  1. 1College of Life Sciences, Northeast Forestry University, Harbin 150040, China
    2State-owned Forest General Factory of Yongji County, Jilin 132000, China
    3Siping State-owned Farm Shilingzi Larch Nation Improved Seed Base, Siping 136000, China
  • Received:2020-02-26 Accepted:2020-06-05 Online:2020-09-01 Published:2020-09-03
  • Contact: Xiangling You

Abstract: In this study, immature zygotic embryos of Larix olgensis were used as explants to induce embryogenic callus and optimize the regeneration system from somatic embryogenesis. The rejuvenation and preservation of the embryogenic potential of embryogenic callus, the somatic embryogenesis and plant regeneration were investigated through adjusting the nutrition and plant growth regulator. The results showed that the generation rates of embryogenic callus were significantly different among different lines. Under the conditions of S+0.2 mg·L -1NAA+0.5 mg·L -1BA+0.5 mg·L -1KT+0.5 g·L -1glutamine+0.5 g·L -1hydrolyzed casein+30 g·L -1sucrose and 3.0 g·L -1vegetable gel, the embryogenic potential of embryonic callus could be recovered and maintained for a long time. Somatic embryogenesis were induced from embryonic callus cultured in S+20 mg·L -1ABA+60 g·L -1PEG4000+60 g·L -1sucrose and 3.0 g·L -1vegetable gel for 6 weeks, and the generation rate of somatic embryo reached 100%. The normal somatic embryos were first cultured for 2 weeks under the conditions of WPM+6 mg·L -1phloglucinol+1.0 g·L -1active carbon+3.0 mg·L -1VB1+20 g·L -1sucrose and 3.0 g·L -1vegetable gel, and then transferred to B5+0.4 mg·L -1NAA+1.0 mg·L -1IBA+0.5 mg·L -1GA3+2.0 mg·L -1VB1+1.0 g·L -1active carbon+20 g·L -1sucrose and 3.0 g·L -1vegetable gel. After 2 weeks, somatic embryo plantlets with cotyledon stretch, hypocotyl elongation and normal root system were observed. This study established a method for the recovery and maintenance of embryogenic callus from larch, and further optimized the somatic embryogenic pathway, which will lay a foundation for the rapid breeding and genetic improvement of L. olgensis.

Key words: Larix olgensis, embryogenic callus, embryogenic recovery, optimization of somatic embryogenesis