Chinese Bulletin of Botany ›› 2010, Vol. 45 ›› Issue (05): 609-614.DOI: 10.3969/j.issn.1674-3466.2010.05.011

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Regulating the Cell Growth and Shoot Induction of Crocus sativus Embryogenic Callus by Rare Earth Elements

Shuan Chen1,2, Xiaodong Wang1, Bing Zhao1*, Yuchun Wang1*   

  1. 1The State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;

    2The China National Center for Biotechnology Development, Ministry of Science and Technology, Beijing 100036, China
  • Received:2009-10-23 Revised:2009-12-23 Online:2010-09-01 Published:2010-09-20
  • Contact: Bing Zhao, Yuchun Wang

Abstract: Rare earth elements were used to promote the growth and induction of embryogenic callus of Crocus sativus by two-stage method for its micropropagation in vitro and then to solve the problems of its limited availability. For promoting cell growth, embryogenic calli were cultured on B5 solid medium supplemented with 0.25 mg·L–1 NAA, 3 mg·L–1 6-BA, 400 mg·L–1 CH, and 0.04 mmol·L–1 La3+. The maximal proliferation rate was 12, which was 1.48-fold of that without rare earth elements. For promoting shoot induction, embryogenic calli were cultured on 1/2 B5 solid medium supplemented with 0.25 mg·L–1 NAA, 3 mg·L–1 6-BA, 400 mg·L–1 CH, and 0.06 mmol·L–1 Ce3+. The maximal shoot induction rate was 84.5%, which was 1.81-fold of that without rare earth elements. In this study, rare earth elements could enhance the cell growth and shoot induction rate of embryogenic calli of C. sativus, which would be useful for its micropropagation.