虎杖R2R3-MYB基因家族鉴定及RjMYB62在协调生长与镉胁迫应答中的功能分析

doi:10.11983/CBB25214

摘要/Abstract

摘要： 虎杖（Reynoutria japonica Houtt.）是一种具有快速生长能力和广泛非生物胁迫耐受性的蓼科多年生草本植物，但其协同调控生长与胁迫适应的分子机制仍有待阐明。R2R3-MYB转录因子在植物生长与胁迫应答的调控中起关键作用。本研究从虎杖两个单倍型染色体基因组中鉴定出231个R2R3-MYB基因（HapA 118个，HapE 113个），并通过系统进化分析将其划分为21个亚家族（S1–S27）。基于茎生长及非生物胁迫下的转录组数据，我们筛选出14个与茎生长发育相关且响应紫外线（UV-B）和茉莉酸甲酯（MeJA）处理的候选基因。qRT-PCR分析进一步证实，这14个MYB基因在茎伸长的不同阶段、多种激素处理以及盐、镉（Cd）和锰（Mn）胁迫下均具有响应，说明它们同时参与生长与胁迫应答过程，可能调控二者的平衡。其中，RjMYB62（HuZ0072119.1，为AtMYB62同源基因）在拟南芥中过表达可显著提高对Cd胁迫的耐受性。本研究系统解析了虎杖R2R3-MYB家族的基本特征，揭示了其在生长与胁迫应答交叉调控中的潜在作用，为深入理解虎杖环境适应性的分子基础提供了依据。

关键词: 虎杖, R2R3-MYB基因家族, RjMYB62, 生长, 非生物胁迫, 镉

Abstract: Reynoutria japonica Houtt. Is a perennial herb of the Polygonaceae family with rapid growth ability and wide tolerance to abiotic stress. However, the molecular mechanism by which it synergically regulates growth and stress adaptation remains to be clarified. The R2R3-MYB transcription factor plays a key role in the regulation of plant growth and stress response. In this study, 231 R2R3-MYB genes (118 HapA and 113 HapE) were identified from the genomes of two haplotypes of R. japonica, and they were classified into 21 subfamilies (S1-S27) through phylogenetic analysis. Based on the transcriptome data of stem growth and abiotic stress, we screened out 14 candidate genes related to stem growth and development and responding to ultraviolet (UV-B) and methyl jasmonate (MeJA) treatments. qRT-PCR analysis further confirmed that these 14 MYB genes all responded at different stages of stem elongation, under multiple hormone treatments, and under stress of salt, cadmium (Cd), and manganese (Mn), indicating that they are simultaneously involved in the growth and stress response processes and may regulate the balance between the two. Among them, overexpression of RjMYB62 (HuZ0072119.1, an AtMYB62 homologous gene) in Arabidopsis thaliana can significantly enhance tolerance to Cd stress. This study systematically analyzed the basic characteristics of the R2R3-MYB family of R. japonica, revealed its potential role in the cross-regulation of growth and stress response, and provided a basis for a deeper understanding of the molecular basis of the environmental adaptability of R. japonica.

Key words: Reynoutria japonica, R2R3-MYBs gene family, growth, abiotic stress, RjMYB62, cadmium

王凡红杨宁宁罗兰卢凡吴倩王国英何佳圆李佳萌杨颖丽王红王晓伟. 虎杖R2R3-MYB基因家族鉴定及RjMYB62在协调生长与镉胁迫应答中的功能分析. 植物学报, DOI: 10.11983/CBB25214.

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