虎杖R2R3-MYB基因家族鉴定及RjMYB62在协调生长与镉胁迫应答中的功能分析

doi:10.11983/CBB25214

摘要/Abstract

摘要： 虎杖（Reynoutria japonica Houtt.）是一种具有快速生长能力和广泛非生物胁迫耐受性的蓼科多年生草本植物，但其协同调控生长与胁迫适应的分子机制仍有待阐明。R2R3-MYB转录因子在植物生长与胁迫应答的调控中起关键作用。本研究从虎杖两个单倍型染色体基因组中鉴定出231个R2R3-MYB基因（HapA 118个，HapE 113个），并通过系统进化分析将其划分为21个亚家族（S1–S27）。基于茎生长及非生物胁迫下的转录组数据，我们筛选出14个与茎生长发育相关且响应紫外线（UV-B）和茉莉酸甲酯（MeJA）处理的候选基因。qRT-PCR分析进一步证实，这14个MYB基因在茎伸长的不同阶段、多种激素处理以及盐、镉（Cd）和锰（Mn）胁迫下均具有响应，说明它们同时参与生长与胁迫应答过程，可能调控二者的平衡。其中，RjMYB62（HuZ0072119.1，为AtMYB62同源基因）在拟南芥中过表达可显著提高对Cd胁迫的耐受性。本研究系统解析了虎杖R2R3-MYB家族的基本特征，揭示了其在生长与胁迫应答交叉调控中的潜在作用，为深入理解虎杖环境适应性的分子基础提供了依据。

关键词: 虎杖, R2R3-MYB基因家族, RjMYB62, 生长, 非生物胁迫, 镉

Abstract: Reynoutria japonica Houtt. Is a perennial herb of the Polygonaceae family with rapid growth ability and wide tolerance to abiotic stress. However, the molecular mechanism by which it synergically regulates growth and stress adaptation remains to be clarified. The R2R3-MYB transcription factor plays a key role in the regulation of plant growth and stress response. In this study, 231 R2R3-MYB genes (118 HapA and 113 HapE) were identified from the genomes of two haplotypes of R. japonica, and they were classified into 21 subfamilies (S1-S27) through phylogenetic analysis. Based on the transcriptome data of stem growth and abiotic stress, we screened out 14 candidate genes related to stem growth and development and responding to ultraviolet (UV-B) and methyl jasmonate (MeJA) treatments. qRT-PCR analysis further confirmed that these 14 MYB genes all responded at different stages of stem elongation, under multiple hormone treatments, and under stress of salt, cadmium (Cd), and manganese (Mn), indicating that they are simultaneously involved in the growth and stress response processes and may regulate the balance between the two. Among them, overexpression of RjMYB62 (HuZ0072119.1, an AtMYB62 homologous gene) in Arabidopsis thaliana can significantly enhance tolerance to Cd stress. This study systematically analyzed the basic characteristics of the R2R3-MYB family of R. japonica, revealed its potential role in the cross-regulation of growth and stress response, and provided a basis for a deeper understanding of the molecular basis of the environmental adaptability of R. japonica.

Key words: Reynoutria japonica, R2R3-MYBs gene family, growth, abiotic stress, RjMYB62, cadmium

王凡红杨宁宁罗兰卢凡吴倩王国英何佳圆李佳萌杨颖丽王红王晓伟. 虎杖R2R3-MYB基因家族鉴定及RjMYB62在协调生长与镉胁迫应答中的功能分析. 植物学报, DOI: 10.11983/CBB25214.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB25214

参考文献

Chen J, Yan Z, Li X (2014). Effect of methyl jasmonate on cadmium uptake and antioxidative capacity in Kandelia obovata seedlings under cadmium stress. Ecotoxicol. Environ. Saf. 104, 349-356. Devaiah BN, Madhuvanthi R, Karthikeyan AS, Raghothama KG (2009). Phosphate starvation responses and gibberellic acid biosynthesis are regulated by the MYB62 transcription factor in Arabidopsis. Molecular plant 2 (1), 43-58. Dharmasiri N, Dharmasiri S, Estelle M (2005). The F-box protein TIR1 is an auxin receptor. Nature 435 (7041), 441-445. Du H, Feng B-R, Yang S-S, Huang Y-B, Tang Y-X (2012). The R2R3-MYB transcription factor gene family in maize. PloS one 7 (6), e37463. Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, Lepiniec L (2010). MYB transcription factors in Arabidopsis. Trends Plant Sci. 15 (10), 573-581. Feng Z, Gao B, Qin C, Lian B, Wu J, Wang J (2024). Overexpression of PsMYB62 from Potentilla sericea confers cadmium tolerance in tobacco. Plant Physiol. Biochem. 216, 109146. Gonzalez A, Zhao M, Leavitt JM, Lloyd AM (2008). Regulation of the anthocyanin biosynthetic pathway by the TTG1/bHLH/Myb transcriptional complex in Arabidopsis seedlings. The Plant Journal 53 (5), 814-827. Guo H, Wang Y, Wang L, Hu P, Wang Y, Jia Y, Zhang C, Zhang Y, Zhang Y, Wang C (2017). Expression of the MYB transcription factor gene Bpl MYB 46 affects abiotic stress tolerance and secondary cell wall deposition in Betula platyphylla. Plant Biotechnol. J. 15 (1), 107-121. Han Z, Zhang C, Zhang H, Duan Y, Zou Z, Zhou L, Zhu X, Fang W, Ma Y (2022). CsMYB transcription factors participate in jasmonic acid signal transduction in response to cold stress in tea plant (Camellia sinensis). Plants 11 (21), 2869. He Y, Li W, Lv J, Jia Y, Wang M, Xia G (2012). Ectopic expression of a wheat MYB transcription factor gene, TaMYB73, improves salinity stress tolerance in Arabidopsis thaliana. J. Exp. Bot. 63 (3), 1511-1522. Jin G, Ma P-F, Wu X, Gu L, Long M, Zhang C, Li D-Z (2021). New genes interacted with recent whole-genome duplicates in the fast stem growth of bamboos. Mol. Biol. Evol. 38 (12), 5752-5768. Ju L-x, Lei X, Zhao C-z, Shu H-y, Wang Z-w, Cheng S-h (2020). Identification of MYB family genes and its relationship with pungency of pepper. Jung C, Seo JS, Han SW, Koo YJ, Kim CH, Song SI, Nahm BH, Do Choi Y, Cheong J-J (2008). Overexpression of AtMYB44 enhances stomatal closure to confer abiotic stress tolerance in transgenic Arabidopsis. Plant Physiol. 146 (2), 623. Kagale S, Rozwadowski K (2011). EAR motif-mediated transcriptional repression in plants: an underlying mechanism for epigenetic regulation of gene expression. Epigenetics 6 (2), 141-146. Kang L, Teng Y, Cen Q, Fang Y, Tian Q, Zhang X, Wang H, Zhang X, Xue D (2022). Genome-wide identification of R2R3-MYB transcription factor and expression analysis under abiotic stress in rice. Plants 11 (15), 1928. Ko JH, Kim WC, Han KH (2009). Ectopic expression of MYB46 identifies transcriptional regulatory genes involved in secondary wall biosynthesis in Arabidopsis. The Plant Journal 60 (4), 649-665. Li J, Liu H, Yang C, Wang J, Yan G, Si P, Bai Q, Lu Z, Zhou W, Xu L (2020). Genome-wide identification of MYB genes and expression analysis under different biotic and abiotic stresses in Helianthus annuus L. Industrial Crops and Products 143, 111924. Li L, Cheng Z, Ma Y, Bai Q, Li X, Cao Z, Wu Z, Gao J (2018). The association of hormone signalling genes, transcription and changes in shoot anatomy during moso bamboo growth. Plant Biotechnol. J. 16 (1), 72-85. Li Y, Ding L, Zhou M, Chen Z, Ding Y, Zhu C (2023). Transcriptional regulatory network of plant cadmium stress response. International Journal of Molecular Sciences 24 (5), 4378. Li Y, Zhang J, Wang S, Liu Y, Yang M, Huang Y (2024). Genome-wide identification of the Pyrus R2R3-MYB gene family and PhMYB62 regulation analysis in Pyrus hopeiensis flowers at low temperature. International Journal of Biological Macromolecules 257, 128611. Lin F, Chen J, Wang X, Ma H, Liang S, Hu H, Fan H, Wu Z, Chai T, Wang H (2024). Combined analysis of Polygonum cuspidatum transcriptome and metabolome revealed that PcMYB62, a transcription factor, responds to methyl jasmonate and inhibits resveratrol biosynthesis. International Journal of Biological Macromolecules 270, 132450. Liu C, Xie T, Chen C, Luan A, Long J, Li C, Ding Y, He Y (2017). Genome-wide organization and expression profiling of the R2R3-MYB transcription factor family in pineapple (Ananas comosus). BMC Genomics 18 (1), 503. Lowe S, Browne M, Boudjelas S, De Poorter M (2000). 100 of the world's worst invasive alien species: a selection from the global invasive species database (Vol. 12). Invasive Species Specialist Group Auckland. Mandaokar A, Browse J (2009). MYB108 acts together with MYB24 to regulate jasmonate-mediated stamen maturation in Arabidopsis. Plant Physiol. 149 (2), 851-862. McCarthy RL, Zhong R, Fowler S, Lyskowski D, Piyasena H, Carleton K, Spicer C, Ye Z-H (2010). The poplar MYB transcription factors, PtrMYB3 and PtrMYB20, are involved in the regulation of secondary wall biosynthesis. Plant and Cell Physiology 51 (6), 1084-1090. Murrell C, Gerber E, Krebs C, Parepa M, Schaffner U, Bossdorf O (2011). Invasive knotweed affects native plants through allelopathy. Am. J. Bot. 98 (1), 38-43. Petroni K, Falasca G, Calvenzani V, Allegra D, Stolfi C, Fabrizi L, Altamura MM, Tonelli C (2008). The AtMYB11 gene from Arabidopsis is expressed in meristematic cells and modulates growth in planta and organogenesis in vitro. J. Exp. Bot. 59 (6), 1201-1213. Ren Z, Qiu F, Wang Y, Yu W, Liu C, Sun Y, Wang Y, Zhang X, Xing S, Tao S (2020). Network analysis of transcriptome and LC‐MS reveals a possible biosynthesis pathway of anthocyanins in dendrobium officinale. BioMed Research International 2020 (1), 6512895. Stracke R, Werber M, Weisshaar B (2001). The R2R3-MYB gene family in Arabidopsis thaliana. Curr. Opin. Plant Biol. 4 (5), 447-456. Sun M, Qiao H-X, Yang T, Zhao P, Zhao J-H, Luo J-M, Liu F-F, Xiong A-S (2024). DcMYB62, a transcription factor from carrot, enhanced cadmium tolerance of Arabidopsis by inducing the accumulation of carotenoids and hydrogen sulfide. Plant Physiol. Biochem. 216, 109114. Tan H, Man C, Xie Y, Yan J, Chu J, Huang J (2019). A crucial role of GA-regulated flavonol biosynthesis in root growth of Arabidopsis. Molecular plant 12 (4), 521-537. VanWallendael A, Alvarez M, Franks SJ (2021). Patterns of population genomic diversity in the invasive Japanese knotweed species complex. Am. J. Bot. 108 (5), 857-868. Wan J, Wang R, Zhang P, Sun L, Ju Q, Huang H, Lü S, Tran L-S, Xu J (2021). MYB70 modulates seed germination and root system development in Arabidopsis. Iscience 24 (11), Wang F, Li M, Liu Z, Li W, He Q, Xing L, Xiao Y, Wang M, Wang Y, Du C (2025). The mixed auto‐/allooctoploid genome of Japanese knotweed (Reynoutria japonica) provides insights into its polyploid origin and invasiveness. The Plant Journal 121 (4), e70005. Wang X, Hu H, Wu Z, Fan H, Wang G, Chai T, Wang H (2021). Tissue-specific transcriptome analyses reveal candidate genes for stilbene, flavonoid and anthraquinone biosynthesis in the medicinal plant Polygonum cuspidatum. BMC Genomics 22 (1), 353. Wang Y, Wu J, Li J, Liu B, Wang D, Gao C (2023). The R2R3-MYB transcription factor ThRAX2 recognized a new element MYB-T (CTTCCA) to enhance cadmium tolerance in Tamarix hispida. Plant Sci. 329, 111574. Woodger FJ, Millar A, Murray F, Jacobsen JV, Gubler F (2003). The role of GAMYB transcription factors in GA-regulated gene expression. J. Plant Growth Regul. 22 (2), 176-184. Yang A, Dai X, Zhang W-H (2012). A R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice. J. Exp. Bot. 63 (7), 2541-2556. Yang J, Zhang B, Gu G, Yuan J, Shen S, Jin L, Lin Z, Lin J, Xie X (2022). Genome-wide identification and expression analysis of the R2R3-MYB gene family in tobacco (Nicotiana tabacum L.). BMC Genomics 23 (1), 432. Yang X, Li J, Guo T, Guo B, Chen Z, An X (2021). Comprehensive analysis of the R2R3-MYB transcription factor gene family in Populus trichocarpa. Industrial Crops and Products 168, 113614. Zhang D, Zhou H, Zhang Y, Zhao Y, Zhang Y, Feng X, Lin H (2025). Diverse roles of MYB transcription factors in plants. Journal of Integrative Plant Biology 67 (3), 539-562. Zhang H, Li C, Kwok S-T, Zhang Q-W, Chan S-W (2013). A review of the pharmacological effects of the dried root of Polygonum cuspidatum (Hu Zhang) and its constituents. Evidence‐Based Complementary and Alternative Medicine 2013 (1), 208349. Zhang L, Ma H (2012). Complex evolutionary history and diverse domain organization of SET proteins suggest divergent regulatory interactions. New Phytol. 195 (1), 248-263. Zhang P, Wang R, Ju Q, Li W, Tran L-SP, Xu J (2019). The R2R3-MYB transcription factor MYB49 regulates cadmium accumulation. Plant Physiol. 180 (1), 529-542. Zhang Y, Zheng L, Zheng Y, Zhou C, Huang P, Xiao X, Zhao Y, Hao X, Hu Z, Chen Q (2019). Assembly and annotation of a draft genome of the medicinal plant Polygonum cuspidatum. Frontiers in plant science 10, 1274. Zhang Z, Zhang T, Lu L, Qiu S, Huang Z, Wang Y, Chen X, Li L, Sun Y, Zhang R (2024). Synergistic interaction between brassinosteroid and jasmonate pathways in rice response to cadmium toxicity. Sci. Total Environ. 954, 176369. Zhang ZB, Zhu J, Gao JF, Wang C, Li H, Li H, Zhang HQ, Zhang S, Wang DM, Wang QX (2007). Transcription factor AtMYB103 is required for anther development by regulating tapetum development, callose dissolution and exine formation in Arabidopsis. The Plant Journal 52 (3), 528-538. Zhao Y, Xing L, Wang X, Hou YJ, Gao J, Wang P, Duan CG, Zhu X, Zhu JK (2014). The ABA receptor PYL8 promotes lateral root growth by enhancing MYB77-dependent transcription of auxin-responsive genes. Sci Signal 7 (328), ra53. Zhong R, Lee C, McCarthy RL, Reeves CK, Jones EG, Ye Z-H (2011). Transcriptional activation of secondary wall biosynthesis by rice and maize NAC and MYB transcription factors. Plant and Cell Physiology 52 (10), 1856-1871.

[1]	吴然然, 郑雪莹, 王宇, 张怡, 贾琪源, 袁星星, 杜吉到, 陈新. 植物TBP及其相关因子（TAFs）的分子功能研究进展[J]. 植物学报, 2026, 61(6): 1-0.
[2]	韩文昊, 王延平. 工程纳米材料调控植物生长和抗逆性的机理研究进展[J]. 植物学报, 2026, 61(6): 1-0.
[3]	虞健翔, 蔡雯, 陈心亦, 刘烯烯, 于茂桢, 余灯雄, 戴绍军, 李丹丹, 于晶. 苔藓植物蛋白质组学研究进展[J]. 植物学报, 2026, 61(5): 729-740.
[4]	刘欣宇, 戴思兰. 生长素响应因子(ARFs)介导的植物花器官发育调控网络与作用机制[J]. 植物学报, 2026, 61(3): 369-385.
[5]	熊良林, 梁国鲁, 郭启高, 景丹龙. 基因可变剪接调控植物响应非生物胁迫研究进展[J]. 植物学报, 2025, 60(3): 435-448.
[6]	李青洋, 刘翠, 何李, 彭姗, 马嘉吟, 胡子祎, 刘宏波. 甘蓝型油菜BnaA02.CPSF6基因的克隆及功能分析(长英文摘要）[J]. 植物学报, 2025, 60(1): 62-73.
[7]	王亚萍, 包文泉, 白玉娥. 单细胞转录组学在植物生长发育及胁迫响应中的应用进展[J]. 植物学报, 2025, 60(1): 101-113.
[8]	王涛, 冯敬磊, 张翠. 高温胁迫影响玉米生长发育的分子机制研究进展[J]. 植物学报, 2024, 59(6): 963-977.
[9]	杜庆国, 李文学. lncRNA调控玉米生长发育和非生物胁迫研究进展[J]. 植物学报, 2024, 59(6): 950-962.
[10]	闫恒宇, 李朝霞, 李玉斌. 高温对玉米生长的影响及中国耐高温玉米筛选研究进展[J]. 植物学报, 2024, 59(6): 1007-1023.
[11]	杨娟, 赵月磊, 陈晓远, 王宝宝, 王海洋. 玉米开花期调控机理及育种应用[J]. 植物学报, 2024, 59(6): 912-931.
[12]	周文杰, 张文瀚, 贾玮, 许自成, 黄五星. 植物miRNA响应非生物胁迫研究进展[J]. 植物学报, 2024, 59(5): 810-833.
[13]	路笃贤, 张严妍, 刘艳, 李岩竣, 左新秀, 林金星, 崔亚宁. 非编码RNA在植物生长发育及逆境响应中的研究进展[J]. 植物学报, 2024, 59(5): 709-725.
[14]	周玉滢, 陈辉, 刘斯穆. 植物非典型Aux/IAA蛋白应答生长素研究进展[J]. 植物学报, 2024, 59(4): 651-658.
[15]	仲昭暄, 张冬瑞, 李璐, 苏颖, 王黛宁, 王泽冉, 刘洋, 常缨. 香鳞毛蕨dfr-miR160a和靶基因DfARF10的生物信息学及表达模式分析[J]. 植物学报, 2024, 59(1): 22-33.