植物miRNA响应非生物胁迫研究进展

doi:10.11983/CBB24020

摘要/Abstract

摘要： 干旱、极端温度、盐和重金属等非生物胁迫导致植物产量和品质下降。miRNA是一类长约20-24个核苷酸的内源性非编码小分子RNA, 通过形成miRNA介导的沉默复合物(RISC)剪切靶mRNA并抑制靶基因的翻译, 在转录后水平负调控真核生物基因表达。高通量测序技术的快速发展使得植物物种中大量响应非生物胁迫的miRNA得到鉴定和表征。非生物胁迫下植物miRNA与其靶基因结合, 构成了控制各种生命活动的大型基因调控网络, 包括生长发育、营养吸收与分配、信号转导与氧化应激, 从而提高植物的抗逆性。深入理解miRNA的功能及其调控机制对于通过基因工程进行作物改良和抗逆育种至关重要。该文综述了近年来miRNA的生物合成及其作用机制研究进展, 重点探讨了参与调控植物响应非生物胁迫miRNA的鉴定及功能, 并展望了该领域可能的研究方向。

关键词: 植物, miRNA, 非生物胁迫, 作用机制

Abstract: Abiotic stresses such as drought, extreme temperatures, salinity, and heavy metals can cause a decrease in plant yield and quality. miRNAs are a class of endogenous non-coding small RNA with a length of about 20-24 nucleotides. By forming miRNA-mediated silencing complexes (RISCs), they cleave target mRNAs and inhibit the translation of target genes, negatively regulating eukaryotic gene expression at the post-transcriptional level. The development of high-throughput sequencing technology has enabled the identification and characterization of a large number of miRNAs that respond to abiotic stress in various plant species. Under abiotic stress, plant miRNAs bind to their target genes, forming a large gene regulatory network that controls various life activities, including growth and development, nutrient absorption and distribution, signal transduction, and oxidative stress, thereby improving plant stress resistance. Understanding the function and regulatory mechanisms of miRNAs is crucial for crop improvement and stress-resistant breeding through genetic engineering. This review summarized the advances in the biosynthesis and mechanisms of plant miRNAs in recent years, with emphasis on the identification and function of miRNAs involved in regulating plant response to abiotic stress. Possible future research directions in this field are also discussed.

Key words: plant, miRNA, abiotic stress, action mechanism

周文杰, 张文瀚, 贾玮, 许自成, 黄五星. 植物miRNA响应非生物胁迫研究进展. 植物学报, 2024, 59(5): 810-833.

Wenjie Zhou, Wenhan Zhang, Wei Jia, Zicheng Xu, Wuxing Huang. Advances in Plant miRNAs Responses to Abiotic Stresses. Chinese Bulletin of Botany, 2024, 59(5): 810-833.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB24020

https://www.chinbullbotany.com/CN/Y2024/V59/I5/810

图/表 5

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名称	取样部位	胁迫处理类型和时间	miRNA差异表达数量	参考文献
拟南芥 (Arabidopsis thaliana)	全幼苗	200 mmol·L^-1甘露醇7天(干旱胁迫)	123	Pegler et al., 2019
	全幼苗	昼夜温度为32°C/28°C 7天(高温胁迫)	121	Pegler et al., 2019
	全幼苗	150 mmol·L^-1氯化钠7天(盐胁迫)	118	Pegler et al., 2019
	叶片通气组织	放置4°C下4小时(低温胁迫)	93	Tiwari et al., 2020
水稻 (Oryza sativa)	叶片组织	放置4°C下5小时(低温胁迫)	23	Shen et al., 2010
	叶片	不浇水直至出现典型症状(干旱胁迫)	64	Cheah et al., 2015
	茎	不浇水直至出现典型症状(干旱胁迫)	71	Cheah et al., 2015
	全幼苗	200 mmol·L^-1氯化钠2天(盐胁迫)	164	Chen et al., 2023
	具有乳熟期颖果的小穗	昼夜温度为38°C/22°C 6小时(高温胁迫)	96	Payne et al., 2023
	全幼苗	昼夜温度为10°C/8°C 5天(低温胁迫)	26	Zhao et al., 2022b
玉米 (Zea mays)	每株第4片叶	昼夜温度为25°C/4°C, 直至该叶片完全成熟(低温胁迫)	65	Aydinoglu, 2020
	每株第10片叶中部	昼夜温度为40°C/25°C 5天(高温胁迫)	102	Zhang et al., 2019
	叶片	16%聚乙二醇24小时(干旱胁迫)	68	Wei et al., 2009
	根系	200 mmol·L^-1氯化钠24小时(盐胁迫)	98	Ding et al., 2009
马铃薯 (Solanum tuberosum)	第3片完全展开的叶片	放置0°C下4小时(低温胁迫)	136	Yan et al., 2021
	全植株	去除培养液6小时(干旱胁迫)	230	Shin et al., 2017
	叶片组织	放置35°C下1小时(高温胁迫)	204	张国栋, 2021
	根系	50 mmol·L^-1 NaHCO₃ 24小时(盐胁迫)	169	康益晨, 2021
番茄(S. lycopersicum)	长度为6.0-6.5 mm的花蕾雄蕊	昼夜温度为35°C/30°C 2天(高温胁迫)	69	Pan et al., 2017
	长度为6.0-6.5 mm的花蕾雌蕊	昼夜温度为35°C/30°C 12天(高温胁迫)	30	Pan et al., 2017
	根系	5%聚乙二醇7天(干旱胁迫)	101	Candar-Cakir et al., 2016
	茎顶端第3片叶	放置4°C下48小时(低温胁迫)	49	Cao et al., 2014
	根系	200 mmol·L^-1氯化钠12小时(盐胁迫)	145	Wang et al., 2021d
烟草 (Nicotiana tabacum)	叶片	放置6°C下24小时(低温胁迫)	25	Hu et al., 2019
	叶片	不浇水10天(干旱胁迫)	32	Chen et al., 2017
	叶片	100 mmol·L^-1氯化钠24小时(盐胁迫)	33	Xu et al., 2019
小麦 (Triticum aestivum)	幼穗	放置0°C下48小时(低温胁迫)	39	Song et al., 2017
	叶片组织	放置37°C下5天(高温胁迫)	79	Ragupathy et al., 2016
	旗叶	保持土壤含水率为田间容量的一半(6%) (干旱胁迫)	54	Liu et al., 2020
	全植株	150 mmol·L^-1氯化钠24小时(盐胁迫)	222	Feng et al., 2017
	根和叶组织	脱水处理8小时(干旱胁迫)	141	Kantar et al., 2011
	冠状组织	自然条件下冬季生育期(低温胁迫)	92	Wang et al., 2021c

名称	取样部位	胁迫处理类型和时间	miRNA差异表达数量	参考文献
拟南芥 (Arabidopsis thaliana)	全幼苗	200 mmol·L^-1甘露醇7天(干旱胁迫)	123	Pegler et al., 2019
	全幼苗	昼夜温度为32°C/28°C 7天(高温胁迫)	121	Pegler et al., 2019
	全幼苗	150 mmol·L^-1氯化钠7天(盐胁迫)	118	Pegler et al., 2019
	叶片通气组织	放置4°C下4小时(低温胁迫)	93	Tiwari et al., 2020
水稻 (Oryza sativa)	叶片组织	放置4°C下5小时(低温胁迫)	23	Shen et al., 2010
	叶片	不浇水直至出现典型症状(干旱胁迫)	64	Cheah et al., 2015
	茎	不浇水直至出现典型症状(干旱胁迫)	71	Cheah et al., 2015
	全幼苗	200 mmol·L^-1氯化钠2天(盐胁迫)	164	Chen et al., 2023
	具有乳熟期颖果的小穗	昼夜温度为38°C/22°C 6小时(高温胁迫)	96	Payne et al., 2023
	全幼苗	昼夜温度为10°C/8°C 5天(低温胁迫)	26	Zhao et al., 2022b
玉米 (Zea mays)	每株第4片叶	昼夜温度为25°C/4°C, 直至该叶片完全成熟(低温胁迫)	65	Aydinoglu, 2020
	每株第10片叶中部	昼夜温度为40°C/25°C 5天(高温胁迫)	102	Zhang et al., 2019
	叶片	16%聚乙二醇24小时(干旱胁迫)	68	Wei et al., 2009
	根系	200 mmol·L^-1氯化钠24小时(盐胁迫)	98	Ding et al., 2009
马铃薯 (Solanum tuberosum)	第3片完全展开的叶片	放置0°C下4小时(低温胁迫)	136	Yan et al., 2021
	全植株	去除培养液6小时(干旱胁迫)	230	Shin et al., 2017
	叶片组织	放置35°C下1小时(高温胁迫)	204	张国栋, 2021
	根系	50 mmol·L^-1 NaHCO₃ 24小时(盐胁迫)	169	康益晨, 2021
番茄(S. lycopersicum)	长度为6.0-6.5 mm的花蕾雄蕊	昼夜温度为35°C/30°C 2天(高温胁迫)	69	Pan et al., 2017
	长度为6.0-6.5 mm的花蕾雌蕊	昼夜温度为35°C/30°C 12天(高温胁迫)	30	Pan et al., 2017
	根系	5%聚乙二醇7天(干旱胁迫)	101	Candar-Cakir et al., 2016
	茎顶端第3片叶	放置4°C下48小时(低温胁迫)	49	Cao et al., 2014
	根系	200 mmol·L^-1氯化钠12小时(盐胁迫)	145	Wang et al., 2021d
烟草 (Nicotiana tabacum)	叶片	放置6°C下24小时(低温胁迫)	25	Hu et al., 2019
	叶片	不浇水10天(干旱胁迫)	32	Chen et al., 2017
	叶片	100 mmol·L^-1氯化钠24小时(盐胁迫)	33	Xu et al., 2019
小麦 (Triticum aestivum)	幼穗	放置0°C下48小时(低温胁迫)	39	Song et al., 2017
	叶片组织	放置37°C下5天(高温胁迫)	79	Ragupathy et al., 2016
	旗叶	保持土壤含水率为田间容量的一半(6%) (干旱胁迫)	54	Liu et al., 2020
	全植株	150 mmol·L^-1氯化钠24小时(盐胁迫)	222	Feng et al., 2017
	根和叶组织	脱水处理8小时(干旱胁迫)	141	Kantar et al., 2011
	冠状组织	自然条件下冬季生育期(低温胁迫)	92	Wang et al., 2021c

miRNA	靶基因	功能	参考文献
miR156	SPLs	调节植物生长时期的转变, 促进生长发育和果实成熟, 增强光合作用, 提高作物产量	芦梦荻, 2021; He et al., 2022
miR159	MYB33、MYB65和MYB101	促进种子萌发, 转导激素信号, 调节气孔数量	Guo et al., 2021
miR166	HD-ZipIII家族	维持渗透平衡, 改善养分吸收, 促进根系生长, 调节脱落酸和生长素信号转导	Li et al., 2020b; Yadav et al., 2023
miR169	NF-YA家族	调节种子发芽和根系伸长, 提高光合速率	Jiao et al., 2021; Wang et al., 2022
miR171	SCL6	促进植物根系发育, 转导光化学信号和激素信号	Sun et al., 2022; Um et al., 2022; 冯继鹏等, 2023
miR319	TCP4/14/21和PCF5/6/8	控制叶片形态, 调节植物发育和昼夜节律	Fang et al., 2021; Lu et al., 2023
miR390	TAS3和ARF2/3/4	控制侧根发育, 转导激素信号	Lin et al., 2015; Vanesa Hobecker et al., 2017; Xia et al., 2017; Chu et al., 2022; Dong et al., 2022
miR393	TIR1和AFBs	转导生长素信号, 调节气孔开闭	Yuan et al., 2019b
miR395	SULTR2;1、SULTR2;2和APS1/3/4	调节植物硫代谢, 促进光合作用, 减少氧化损伤	Sunkar et al., 2007; Zhang et al., 2013
miR396	GRFs	促进营养吸收, 调节生长发育, 调节氮代谢	何彬等, 2022
miR397	LACs	调控木质素合成及细胞壁厚度	Huang et al., 2021; 吴鹏等, 2022
miR408	LACs和铜氧还蛋白编码基因	调节铜稳态, 参与光合作用和光形态发生, 维持活性氧平衡	Hu et al., 2023a; Qin et al., 2023
miR444	MADS23/25/27/57/61	调控营养元素吸收, 参与激素信号转导, 调节根系发育	黄圣, 2022; 李鲁华等, 2022
miR528	LAC3/5和AO	调节抗氧化酶活性, 减少氧化损伤	Guo et al., 2023
miR827	SPX结构域基因和WRKY48	调节营养发育, 控制气孔密度	Lin et al., 2010; Yang et al., 2022a

miRNA	靶基因	功能	参考文献
miR156	SPLs	调节植物生长时期的转变, 促进生长发育和果实成熟, 增强光合作用, 提高作物产量	芦梦荻, 2021; He et al., 2022
miR159	MYB33、MYB65和MYB101	促进种子萌发, 转导激素信号, 调节气孔数量	Guo et al., 2021
miR166	HD-ZipIII家族	维持渗透平衡, 改善养分吸收, 促进根系生长, 调节脱落酸和生长素信号转导	Li et al., 2020b; Yadav et al., 2023
miR169	NF-YA家族	调节种子发芽和根系伸长, 提高光合速率	Jiao et al., 2021; Wang et al., 2022
miR171	SCL6	促进植物根系发育, 转导光化学信号和激素信号	Sun et al., 2022; Um et al., 2022; 冯继鹏等, 2023
miR319	TCP4/14/21和PCF5/6/8	控制叶片形态, 调节植物发育和昼夜节律	Fang et al., 2021; Lu et al., 2023
miR390	TAS3和ARF2/3/4	控制侧根发育, 转导激素信号	Lin et al., 2015; Vanesa Hobecker et al., 2017; Xia et al., 2017; Chu et al., 2022; Dong et al., 2022
miR393	TIR1和AFBs	转导生长素信号, 调节气孔开闭	Yuan et al., 2019b
miR395	SULTR2;1、SULTR2;2和APS1/3/4	调节植物硫代谢, 促进光合作用, 减少氧化损伤	Sunkar et al., 2007; Zhang et al., 2013
miR396	GRFs	促进营养吸收, 调节生长发育, 调节氮代谢	何彬等, 2022
miR397	LACs	调控木质素合成及细胞壁厚度	Huang et al., 2021; 吴鹏等, 2022
miR408	LACs和铜氧还蛋白编码基因	调节铜稳态, 参与光合作用和光形态发生, 维持活性氧平衡	Hu et al., 2023a; Qin et al., 2023
miR444	MADS23/25/27/57/61	调控营养元素吸收, 参与激素信号转导, 调节根系发育	黄圣, 2022; 李鲁华等, 2022
miR528	LAC3/5和AO	调节抗氧化酶活性, 减少氧化损伤	Guo et al., 2023
miR827	SPX结构域基因和WRKY48	调节营养发育, 控制气孔密度	Lin et al., 2010; Yang et al., 2022a