不同钾水平下烟草根内皮层木栓化的适应性发育差异研究

doi:10.11983/CBB24191

植物学报

不同钾水平下烟草根内皮层木栓化的适应性发育差异研究

许耘祥^{1, 2}, 张莉汶^{1, 2}, 王朋¹, 顾迎晨^{1, 2}, Madan Lal Kolhi^{1, 2}, 张标^{1, 2}, 朱莹莹¹, 刘海伟¹^*

¹中国农业科学院烟草研究所/农业农村部烟草生物学与加工重点实验室, 山东青岛 266101; ²中国农业科学院研究生院, 北京 100081

收稿日期:2024-12-09 修回日期:2025-03-04 出版日期:2025-03-18 发布日期:2025-03-18
通讯作者: 刘海伟
基金资助:
山东省自然科学基金面上项目(No.ZR2023MC150)和中国农业科学院科技创新工程项目(No.ASTIP-TRIC03)

Differences in Adaptive Development of Suberin in Tobacco Root Endothelial Layer under Different Potassium Levels

Yunxiang Xu^{1, 2}, Liwen Zhang^{1, 2}, Peng Wang¹, Yingchen Gu^{1, 2}, Madan Lal Kolhi^{1, 2}, Biao Zhang^{1, 2}, Yingying Zhu¹, Haiwei Liu¹*

¹Institute of Tobacco Research of CAAS/Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Qingdao 266101, China; ²Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2024-12-09 Revised:2025-03-04 Online:2025-03-18 Published:2025-03-18
Contact: Haiwei Liu

摘要/Abstract

摘要： 根内皮层分化的质外体屏障在植物抗逆和养分吸收均起到重要作用, 其中木栓层发育成为近年研究的热点。本研究以烟草栽培品种中烟100为材料, 通过0.1–4.0 mmol∙L^–1钾浓度梯度水培实验, 探讨供钾水平对根内皮层木栓化发育的影响及其生理分子机制。结果表明，低钾胁迫(0.1 mmol∙L^–1)显著增强内皮层木栓化: 完全木栓化区域绝对长度由对照的0–2 cm延伸至4–6 cm, 相对占比从0–15.0%提升至33.2–44.3%, 表明木栓化是烟草响应低钾胁迫的关键形态适应机制之一。表型分析显示, 低钾胁迫下植株根系伸长但生物量下降, 地上部与根系钾离子含量及积累量分别减少, 木质部汁液流量及钾离子浓度运输效率降低。内源激素含量检测发现, 低钾胁迫提高根系内源脱落酸含量, 并抑制乙烯和茉莉酸甲酯含量, 形成特异性激素调控网络。转录组数据进一步佐证木栓化发育的分子基础, 木栓质合成与转运相关基因(如CYP86、GPAT、ABCG)及其上游正调控因子MYB36/41/92/93显著上调。因此, 本研究首次阐明烟草低钾胁迫通过ABA介导的激素信号, 调控木栓化发育程序, 为解析作物钾胁迫适应机制提供了全新视角。

关键词: 木栓化, 钾, 根内皮层, 激素

Abstract: INTRODUCTION: The apoplastic barriers differentiated from the root endodermis play an important role in plant stress resistance and nutrient uptake, and the development of suberin lamella has become a hot research topic in recent years.

RATIONALE: Hydroponic experiments with potassium concentration gradients ranging from 0.1 to 4.0 mmol∙L^–1 were conducted, using the cultivated tobacco variety Zhongyan 100 as the experimental material, to explore the effect of different potassium supplies on the endodermal suberization and its physiological and molecular mechanisms.
RESULTS: It was found that low potassium stress (0.1 mmol∙L^–1) significantly enhanced the endodermal suberization: the absolute length of the fully suberized region extended from 0–2 cm in the control to 4–6 cm, and the relative proportion increased from 0–15.0% to 33.2–44.3%. This indicates that suberization is one of the key morphological adaptation mechanisms in tobacco under low potassium stress. Phenotypic analysis showed that under low potassium stress, root elongation increased while plant biomass decreased, and potassium ion content and accumulation in both the aboveground and root parts were reduced. Additionally, the flow rate and potassium ion concentration in the xylem sap decreased, indicating reduced transport efficiency. Endogenous hormone analysis revealed that low potassium stress increased abscisic acid (ABA) content in roots while suppressing ethylene and methyl jasmonate levels, forming a specific hormonal regulatory network. Transcriptome data further supported the molecular basis of suberization development, showing significant upregulation of genes related to suberin synthesis and transport (e.g., CYP86, GPAT, ABCG) and their upstream positive regulatory factors (MYB36, MYB41, MYB92, MYB93).
CONCLUSION: This study is the first to elucidate that low potassium stress regulates the suberization developmental program of tobacco root through ABA-mediated hormonal signaling reprogramming, providing a novel perspective for understanding the adaptation mechanisms of plants to potassium stress.

Fluorescence imaging of transverse sections of endodermis development in tobacco root under three potassium concentrations

Key words: suberization,, potassium,, root endodermis,, hormone

许耘祥, 张莉汶, 王朋, 顾迎晨, Madan Lal Kolhi, 张标, 朱莹莹, 刘海伟. 不同钾水平下烟草根内皮层木栓化的适应性发育差异研究. 植物学报, DOI: 10.11983/CBB24191.

Yunxiang Xu, Liwen Zhang, Peng Wang, Yingchen Gu, Madan Lal Kolhi, Biao Zhang, Yingying Zhu, Haiwei Liu.

Differences in Adaptive Development of Suberin in Tobacco Root Endothelial Layer under Different Potassium Levels

. Chinese Bulletin of Botany, DOI: 10.11983/CBB24191.

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不同钾水平下烟草根内皮层木栓化的适应性发育差异研究

Differences in Adaptive Development of Suberin in Tobacco Root Endothelial Layer under Different Potassium Levels

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