植物适应土壤氮素环境的基因选择: 以水稻为例

doi:10.11983/CBB20208

摘要/Abstract

摘要： 在农业生产过程中, 施用无机氮肥是提高作物产量的基础, 但氮肥过量施加对生态系统和植物发育进程均会产生不利影响。因此, 提高作物氮素利用效率是农业可持续发展的关键。目前, 对重要粮食作物水稻(Oryza sativa)的氮高效研究取得了一系列重要进展, 并克隆到多个调控氮素吸收、转运和代谢的关键基因。然而, 在不断被人工选育的过程中, 水稻适应土壤不同氮素环境的遗传基础尚不清楚。近日, 中国科学院遗传与发育生物学研究所储成才团队通过评估全球不同生态地理区域的水稻种质资源, 以分蘖数对氮的响应(TRN)为指标进行全基因组关联分析, 鉴定到1个TRN关键负调控基因OsTCP19, 发现其启动子区1个29 bp的核苷酸插入缺失多态性(InDel)变异与品种间的TRN差异密切相关。OsTCP19通过抑制其下游分蘖促进基因DLT转录进而负调控TRN, 而OsTCP19自身的转录活性受氮响应因子OsLBD负调控。OsTCP19的不同等位变异在不同水稻品种中被差异选择并固定, 且与当地土壤含氮量显著相关, 是调控水稻氮素适应性的重要遗传基础。该研究揭示了水稻适应土壤氮素环境变化的分子遗传机制, 为水稻氮素高效利用育种提供了新的遗传资源。

关键词: 水稻, 分蘖对氮的响应, 全基因组关联分析, 氮素利用效率, 地理适应性, OsTCP19, OsLBD, DLT

Abstract: Crop productivity relies heavily on inorganic nitrogen (N) fertilization, while excess application of N fertilizers results in detrimental effects on ecosystem and plant developmental process. Thus, the improvement of crop N use efficiency (NUE) is critical for the development of sustainable agriculture. Thus far, significant advances in understanding the regulation of NUE have been achieved in rice (Oryza sativa), one of the most important food crops. Several key transporter and regulatory genes involved in N uptake, translocation, and metabolism have been cloned and characterized in rice. However, the genetic mechanisms underlying the geographic adaptation of rice to the change of local soil N status remain elusive. Recently, a team led by Prof. Chengcai Chu, in Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, evaluated the responses to N supplies of rice germplasm resources collected from different eco-geographical regions worldwide. By performing genome-wide association study on rice tillering response to N (TRN), OsTCP19 is identified as a repressor of TRN, and a 29 bp InDel polymorphism in its promoter determines TRN variations among the rice varieties. OsTCP19 regulates TRN by inhibiting the transcription of DLT, a tiller-promoting gene, whilst the transcription of OsTCP19 itself is controlled by a N responsive suppressor LATERAL ORGAN BOUNDARIES DOMAIN (LBD) protein. Notably, OsTCP19 haplotypes were selected among rice germplasms and correlated with local soil N content. This study not only reveals the genetic basis of geographic adaptation of cultivated rice to the changes of soil N environment, but also provides novel genetic candidates for effective breeding of higher NUE rice cultivars.

Key words: rice (Oryza sativa), tillering response to nitrogen, genome-wide association study, nitrogen use efficiency, geographic adaptation, OsTCP19, OsLBD, DLT

宣伟, 徐国华. 植物适应土壤氮素环境的基因选择: 以水稻为例. 植物学报, 2021, 56(1): 1-5.

Wei Xuan, Guohua Xu. Genomic Basis of Rice Adaptation to Soil Nitrogen Status. Chinese Bulletin of Botany, 2021, 56(1): 1-5.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB20208

https://www.chinbullbotany.com/CN/Y2021/V56/I1/1

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