植物学报 ›› 2024, Vol. 59 ›› Issue (2): 217-230.DOI: 10.11983/CBB23161 cstr: 32102.14.CBB23161
朱超宇1, 胡程翔1, 朱哲楠1, 张芷宁1, 汪理海1, 陈钧1, 李三峰2, 连锦瑾1, 唐璐瑶1, 钟芊芊1, 殷文晶1, 王跃星2,*(), 饶玉春1,*()
收稿日期:
2023-12-04
接受日期:
2024-01-30
出版日期:
2024-03-10
发布日期:
2024-03-10
通讯作者:
* E-mail: 基金资助:
Chaoyu Zhu1, Chengxiang Hu1, Zhenan Zhu1, Zhining Zhang1, Lihai Wang1, Jun Chen1, Sanfeng Li2, Jinjin Lian1, Luyao Tang1, Qianqian Zhong1, Wenjing Yin1, Yuexing Wang2,*(), Yuchun Rao1,*()
Received:
2023-12-04
Accepted:
2024-01-30
Online:
2024-03-10
Published:
2024-03-10
Contact:
* E-mail: 摘要: 水稻(Oryza sativa)穗部性状与产量直接相关, 其相关基因的挖掘与功能解析对于保障国家粮食安全意义重大。以籼稻华占(HZ)和粳稻热研2号(Nekken2)及构建的120个重组自交系(RILs)为实验材料, 测定了穗长、每穗粒数、结实率、柱头外露率及一次枝梗数等穗部性状。结合高密度分子遗传图谱进行QTL定位, 结果共检测到31个QTLs, 分别位于第1、2、3、4、5、6、10和11号染色体上, 其中2个位点的LOD值分别高达5.45与5.28。通过分析筛选QTL区间内可能影响穗部性状的相关基因, 并利用qRT-PCR进行基因表达检测, 发现LOC_Os05g05490、LOC_Os05g06150、LOC_Os03g11700、LOC_Os03g12430、LOC_Os05g28720、LOC_Os05g30890、LOC_Os05g31740和LOC_Os02g17880在双亲间的表达水平差异显著。其中, 前5个基因编码三角状五肽重复蛋白, 而后3个基因编码糖基转移酶。研究挖掘到31个与穗部性状相关的QTLs, 为进一步定位和克隆相关基因, 从而选育高产水稻新品种奠定理论基础。
朱超宇, 胡程翔, 朱哲楠, 张芷宁, 汪理海, 陈钧, 李三峰, 连锦瑾, 唐璐瑶, 钟芊芊, 殷文晶, 王跃星, 饶玉春. 水稻穗部性状QTL定位及候选基因分析. 植物学报, 2024, 59(2): 217-230.
Chaoyu Zhu, Chengxiang Hu, Zhenan Zhu, Zhining Zhang, Lihai Wang, Jun Chen, Sanfeng Li, Jinjin Lian, Luyao Tang, Qianqian Zhong, Wenjing Yin, Yuexing Wang, Yuchun Rao. Mapping of QTLs Associated with Rice Panicle Traits and Candidate Gene Analysis. Chinese Bulletin of Botany, 2024, 59(2): 217-230.
Traits | Primer name | Forward primer (5′-3′) | Reverse primer (5′-3′) |
---|---|---|---|
OsActin | TGGCATCTCAGCACATTCC | TGCACAATGGATGGGTCAGA | |
Panicle length | LOC_Os03g08900 | CTCTTCTTCTGGGTGCTCAT | GATGCCGATGAGGTAGTAGG |
LOC_Os03g10210 | AGTTGCTGAAGCTGAAGGAG | CGCGTACTCACTCATGTAGG | |
LOC_Os03g10520 | GCTCCTTATCCGACTCAAGA | AACCAACTGCTCCACAATTT | |
LOC_Os03g10620 | GACAACCTCGACGCCTAC | ACCTGCTGTATCTCCTCCAG | |
LOC_Os03g11670 | TGTACAACATGCTCCTTTCG | CGAGGTGAGGATGAAGATGT | |
LOC_Os03g12120 | TGCAGATTCTTCTGGACAAA | AACTGAAGATAGCGGGACTG | |
Grain number per panicle | LOC_Os05g30890 | GAATCTCGGTGGCATCAAAT | TCATGCTGAGAGCCATCTTG |
LOC_Os05g31040 | CTGACATTGCCACAACCATC | GTTCTCCTCCTGAAGCATCG | |
LOC_Os05g31280 | GAACAACTCGTCGTCGTCGT | GACTGCTTGTTGCCGTAGGT | |
LOC_Os05g31380 | CAGTTCCATCCGGTGCTATC | CATCGTATGCTGGCTGGAT | |
LOC_Os05g31740 | GACTGGTACACCGACCTGCT | TAGGACGCCACTTTCCTCTG | |
Seed-setting rate | LOC_Os02g50240 | CACCAACAAGAGGCACAATG | TCAACAATATCACGCCCAAA |
LOC_Os02g50740 | GTTCCCTTCCTTCCCTTGAC | TGCTGCTCCTCCTGACTTCT | |
LOC_Os02g52990 | CGATCGCCTACCTCTACCAC | CACGGGGAGAAGGAGTAGTG | |
LOC_Os02g53680 | GAAATTGAGGATGCCCAGAA | CACAGGAAAGATCCCACGTT | |
LOC_Os02g54910 | CCAGAAATCGAAGGAACCAA | CTCGCGGAAGTATCCATCAT | |
LOC_Os02g56140 | GCTCCAGGTCCAGATGTTGT | CGGTTGCATTAGGAACACCT | |
Stigma exsertion rate | LOC_Os05g02870 | CAAGCTCAAGGTGCTCGAC | TTGTTCTTGCTGCCAAAGTG |
LOC_Os05g04160 | GTGCGGGTCTACAATCACCT | ACTATCGTCCCATGGCTCAC | |
LOC_Os05g04770 | ATCCGTCTGTGTTTCGATCC | TATTCGTCCATCCGAAAAGC | |
LOC_Os05g05280 | GCCACCAGCCTAATCATTGT | GCACAAGAACCGACAAGACA | |
LOC_Os05g05490 | TGGAGTTCTTCCAAGGATGG | GCAACACATAAGCAGCCGTA | |
LOC_Os05g06150 | GGAAATTGTTTGGGGTGATG | GCATCCCTTGCTTCAACAAT | |
LOC_Os05g08350 | ATCCCCGGAGATTCGATTAC | AGTAAATTTCCACCGCAACG | |
Number of primary branches | LOC_Os05g09740 | AACATCATCGGCTGGAAGAC | TGCCTGATCATATGGCTGAA |
LOC_Os05g10210 | AACATCATCGGCTGGAAGAC | GCGTGGTCGTATGGCTTAGT | |
LOC_Os05g10370 | CTGCTCCTCCTCCTCTTCCT | ATCACGTTGTGTGCCTCCAC | |
LOC_Os05g10690 | CTTGACAAGCTCGGAAAAGG | TGCAGTTCAGATGCAGATCC | |
LOC_Os05g10770 | TGGTGCTGATCTGGAGACTG | GTCTTCAACATGCCAGCAGA | |
Number of secondary branches | LOC_Os03g11614 | CTCTTCGAGTTCTCCAGCTC | CCAGCTCCTTCATGCTTAGT |
LOC_Os03g11700 | ACTGCTCCTATGCCGTATCG | AGGTACAAGCGGCTTCCTTT | |
LOC_Os03g11790 | TGAATCGGCTCATGTCCATA | GGCAGCATCTTGAAGTGACA | |
LOC_Os03g12430 | GATGACAGGAAGAGGGGTGA | TCATCATCCTCTGCACAAGC | |
LOC_Os03g12940 | ACCCTGCCAGAGCTTGTAGA | TCTGCTGTGCTCACCTCATC | |
LOC_Os05g28720 | TGATCCAAGAGATGGGGAAG | GCATTCTGTCGAACACCTGA | |
Thousand grain weight | LOC_Os02g17880 | TGTACGCCTACTCGAGCATA | ATCCTGAAGTTGTCCTGCAC |
LOC_Os02g18820 | TCTCAGCCTTCAGAACATGA | TCTAGAACTGCCGACATCAA | |
LOC_Os02g19820 | CATGAAAATGGTGATCCAGA | GCTGCGTCGACTTGTAGTAG | |
LOC_Os02g29530 | ACTTCTCGCACCCACTTATC | ATTTTTCCAGAGGCTTTGTG | |
LOC_Os02g30850 | GTGAAGCGCCTCTTCTGC | GAGGGAGCCGTTGATGTG | |
LOC_Os02g32370 | GCAAACCCTGTACCACTTCT | AAGACGCCTTCTCGGTAAC | |
LOC_Os05g11700 | CGGACTCGGTGAGCTATAAT | CAACAAATTTCCTCACACCA | |
Grain length | LOC_Os03g44430 | CAAGGGTCGGAAGACAGTTC | TATCATCCGCATCCTCATCA |
LOC_Os03g44500 | GTGTGTGATGGATGGCTTTG | GTTGGAGTCGGTGGTCTGTT | |
LOC_Os03g45830 | GATGTGGAGGAGGAAGAGCA | GCCGTACCCGTACTGCTG | |
LOC_Os03g46180 | AAGAGGAGGAGGAGGAGCAG | CTTCTTCAGCACGCTGTTCA | |
LOC_Os03g46560 | GGCTTTGTCCAAAAATGGAA | CTGGCAGTCCAAAGACATCA | |
Grain width | LOC_Os02g30910 | TCACCTTTGGCATCTTAGGT | AGGTACATGGCGAGGTAGAC |
LOC_Os06g10950 | GTACCAATCCGCCATGTACC | GCGGTAGGAGATGGAGACAA | |
LOC_Os06g10960 | ACCTCGACCACGACTACACC | GACCCCAGATGTGGTTGTTC | |
LOC_Os06g11270 | GGTTACCAAGAACGGACGAA | CACCTGCGATCCTACCTTGT | |
LOC_Os06g09270 | TACGTGCTGTCCTGGGATCT | AAGGTTTGTGCCCATCTCAG |
表1 qRT-PCR引物序列
Table 1 Primer sequences for qRT-PCR
Traits | Primer name | Forward primer (5′-3′) | Reverse primer (5′-3′) |
---|---|---|---|
OsActin | TGGCATCTCAGCACATTCC | TGCACAATGGATGGGTCAGA | |
Panicle length | LOC_Os03g08900 | CTCTTCTTCTGGGTGCTCAT | GATGCCGATGAGGTAGTAGG |
LOC_Os03g10210 | AGTTGCTGAAGCTGAAGGAG | CGCGTACTCACTCATGTAGG | |
LOC_Os03g10520 | GCTCCTTATCCGACTCAAGA | AACCAACTGCTCCACAATTT | |
LOC_Os03g10620 | GACAACCTCGACGCCTAC | ACCTGCTGTATCTCCTCCAG | |
LOC_Os03g11670 | TGTACAACATGCTCCTTTCG | CGAGGTGAGGATGAAGATGT | |
LOC_Os03g12120 | TGCAGATTCTTCTGGACAAA | AACTGAAGATAGCGGGACTG | |
Grain number per panicle | LOC_Os05g30890 | GAATCTCGGTGGCATCAAAT | TCATGCTGAGAGCCATCTTG |
LOC_Os05g31040 | CTGACATTGCCACAACCATC | GTTCTCCTCCTGAAGCATCG | |
LOC_Os05g31280 | GAACAACTCGTCGTCGTCGT | GACTGCTTGTTGCCGTAGGT | |
LOC_Os05g31380 | CAGTTCCATCCGGTGCTATC | CATCGTATGCTGGCTGGAT | |
LOC_Os05g31740 | GACTGGTACACCGACCTGCT | TAGGACGCCACTTTCCTCTG | |
Seed-setting rate | LOC_Os02g50240 | CACCAACAAGAGGCACAATG | TCAACAATATCACGCCCAAA |
LOC_Os02g50740 | GTTCCCTTCCTTCCCTTGAC | TGCTGCTCCTCCTGACTTCT | |
LOC_Os02g52990 | CGATCGCCTACCTCTACCAC | CACGGGGAGAAGGAGTAGTG | |
LOC_Os02g53680 | GAAATTGAGGATGCCCAGAA | CACAGGAAAGATCCCACGTT | |
LOC_Os02g54910 | CCAGAAATCGAAGGAACCAA | CTCGCGGAAGTATCCATCAT | |
LOC_Os02g56140 | GCTCCAGGTCCAGATGTTGT | CGGTTGCATTAGGAACACCT | |
Stigma exsertion rate | LOC_Os05g02870 | CAAGCTCAAGGTGCTCGAC | TTGTTCTTGCTGCCAAAGTG |
LOC_Os05g04160 | GTGCGGGTCTACAATCACCT | ACTATCGTCCCATGGCTCAC | |
LOC_Os05g04770 | ATCCGTCTGTGTTTCGATCC | TATTCGTCCATCCGAAAAGC | |
LOC_Os05g05280 | GCCACCAGCCTAATCATTGT | GCACAAGAACCGACAAGACA | |
LOC_Os05g05490 | TGGAGTTCTTCCAAGGATGG | GCAACACATAAGCAGCCGTA | |
LOC_Os05g06150 | GGAAATTGTTTGGGGTGATG | GCATCCCTTGCTTCAACAAT | |
LOC_Os05g08350 | ATCCCCGGAGATTCGATTAC | AGTAAATTTCCACCGCAACG | |
Number of primary branches | LOC_Os05g09740 | AACATCATCGGCTGGAAGAC | TGCCTGATCATATGGCTGAA |
LOC_Os05g10210 | AACATCATCGGCTGGAAGAC | GCGTGGTCGTATGGCTTAGT | |
LOC_Os05g10370 | CTGCTCCTCCTCCTCTTCCT | ATCACGTTGTGTGCCTCCAC | |
LOC_Os05g10690 | CTTGACAAGCTCGGAAAAGG | TGCAGTTCAGATGCAGATCC | |
LOC_Os05g10770 | TGGTGCTGATCTGGAGACTG | GTCTTCAACATGCCAGCAGA | |
Number of secondary branches | LOC_Os03g11614 | CTCTTCGAGTTCTCCAGCTC | CCAGCTCCTTCATGCTTAGT |
LOC_Os03g11700 | ACTGCTCCTATGCCGTATCG | AGGTACAAGCGGCTTCCTTT | |
LOC_Os03g11790 | TGAATCGGCTCATGTCCATA | GGCAGCATCTTGAAGTGACA | |
LOC_Os03g12430 | GATGACAGGAAGAGGGGTGA | TCATCATCCTCTGCACAAGC | |
LOC_Os03g12940 | ACCCTGCCAGAGCTTGTAGA | TCTGCTGTGCTCACCTCATC | |
LOC_Os05g28720 | TGATCCAAGAGATGGGGAAG | GCATTCTGTCGAACACCTGA | |
Thousand grain weight | LOC_Os02g17880 | TGTACGCCTACTCGAGCATA | ATCCTGAAGTTGTCCTGCAC |
LOC_Os02g18820 | TCTCAGCCTTCAGAACATGA | TCTAGAACTGCCGACATCAA | |
LOC_Os02g19820 | CATGAAAATGGTGATCCAGA | GCTGCGTCGACTTGTAGTAG | |
LOC_Os02g29530 | ACTTCTCGCACCCACTTATC | ATTTTTCCAGAGGCTTTGTG | |
LOC_Os02g30850 | GTGAAGCGCCTCTTCTGC | GAGGGAGCCGTTGATGTG | |
LOC_Os02g32370 | GCAAACCCTGTACCACTTCT | AAGACGCCTTCTCGGTAAC | |
LOC_Os05g11700 | CGGACTCGGTGAGCTATAAT | CAACAAATTTCCTCACACCA | |
Grain length | LOC_Os03g44430 | CAAGGGTCGGAAGACAGTTC | TATCATCCGCATCCTCATCA |
LOC_Os03g44500 | GTGTGTGATGGATGGCTTTG | GTTGGAGTCGGTGGTCTGTT | |
LOC_Os03g45830 | GATGTGGAGGAGGAAGAGCA | GCCGTACCCGTACTGCTG | |
LOC_Os03g46180 | AAGAGGAGGAGGAGGAGCAG | CTTCTTCAGCACGCTGTTCA | |
LOC_Os03g46560 | GGCTTTGTCCAAAAATGGAA | CTGGCAGTCCAAAGACATCA | |
Grain width | LOC_Os02g30910 | TCACCTTTGGCATCTTAGGT | AGGTACATGGCGAGGTAGAC |
LOC_Os06g10950 | GTACCAATCCGCCATGTACC | GCGGTAGGAGATGGAGACAA | |
LOC_Os06g10960 | ACCTCGACCACGACTACACC | GACCCCAGATGTGGTTGTTC | |
LOC_Os06g11270 | GGTTACCAAGAACGGACGAA | CACCTGCGATCCTACCTTGT | |
LOC_Os06g09270 | TACGTGCTGTCCTGGGATCT | AAGGTTTGTGCCCATCTCAG |
图1 水稻重组自交系穗部性状分布 (A) 穗长; (B) 每穗粒数; (C) 结实率; (D) 柱头外露率; (E) 一次枝梗数; (F) 二次枝梗数; (G) 粒长; (H) 粒宽; (I) 千粒重
Figure 1 Distribution of panicle traits in recombinant inbred lines of rice (A) Panicle length; (B) Grain number per panicle; (C) Seed-setting rate; (D) Stigma exsertion rate; (E) Number of primary branches; (F) Number of secondary branches; (G) Grain length; (H) Grain width; (I) Thousand grain weight
图2 父本华占与母本热研2号表型 (A) 成熟期水稻植株表型(bar=10 cm); (B) 成熟期水稻穗部对比(bar=2 cm)
Figure 2 Phenotypes of the male parent HZ and the female parent Nekken2 (A) Phenotype of mature rice plants (bar=10 cm); (B) Comparison of mature rice panicles (bar=2 cm)
Traits | QTL | Chromosome | Physical distance (bp) | Position of support (cM) | Limit of detection (LOD) |
---|---|---|---|---|---|
Panicle length | qPL3.1 | 3 | 4514694-9883047 | 19.35-42.37 | 5.45 |
qPL5.1 | 5 | 16473540-17216667 | 70.62-73.80 | 2.54 | |
qPL11.1 | 11 | 17453046-17570705 | 74.82-75.32 | 2.29 | |
qPL11.2 | 11 | 26791773-26969035 | 114.85-115.61 | 2.61 | |
Grain number per panicle | qGNPP3.1 | 3 | 6470188-15088477 | 27.74-64.68 | 2.21 |
qGNPP5.1 | 5 | 16020128-18858682 | 68.67-80.84 | 2.36 | |
qGNPP5.2 | 5 | 27007193-27230204 | 115.77-116.73 | 2.41 | |
Seed-setting rate | qSSR1.1 | 1 | 7203302-7421463 | 30.88-31.81 | 2.41 |
qSSR2.1 | 2 | 30004559-34415035 | 128.62-147.53 | 3.47 | |
qSSR10.1 | 10 | 5567413-15675357 | 23.87-67.20 | 2.78 | |
Stigma exsertion rate | qSER5.1 | 5 | 926888-4584013 | 3.97-19.65 | 3.25 |
qSER10.1 | 10 | 10115736-11156109 | 43.36-47.82 | 2.41 | |
Number of primary branches | qNPB1.1 | 1 | 25791086-25812159 | 110.56-110.65 | 2.24 |
qNPB5.1 | 5 | 5350624-6230055 | 22.94-26.71 | 2.66 | |
Number of secondary branches | qNSB2.1 | 2 | 34269184-34415035 | 146.90-147.16 | 2.25 |
qNSB3.1 | 3 | 5859763-7034710 | 25.00-30.16 | 3.18 | |
qNSB5.1 | 5 | 14979844-15200902 | 64.21-65.16 | 2.13 | |
qNSB5.2 | 5 | 15944247-17819466 | 68.35-76.39 | 3.06 | |
qNSB6.1 | 6 | 4263281-5187517 | 18.28-22.24 | 2.88 | |
qNSB11.1 | 11 | 4416803-4813122 | 18.93-20.63 | 2.47 | |
Grain length | qGL3.1 | 3 | 24904943-26519283 | 106.76-113.68 | 2.26 |
qGL6.1 | 6 | 23905310-24717124 | 102.48-105.96 | 2.14 | |
Grain width | qGW2.1 | 2 | 7512377-21714903 | 32.20-93.09 | 3.61 |
qGW3.1 | 3 | 10039327-11005670 | 43.04-47.18 | 2.24 | |
qGW4.1 | 4 | 19218278-19642144 | 82.38-84.20 | 3.28 | |
qGW5.1 | 5 | 3697712-6833061 | 15.85-29.29 | 3.46 | |
qGW5.2 | 5 | 28202582-29177297 | 120.90-125.16 | 3.27 | |
qGW6.1 | 6 | 4227455-6069227 | 18.12-26.02 | 3.77 | |
Thousand grain weight | qTGW1.1 | 1 | 6064691-6235269 | 26.00-26.73 | 2.80 |
qTGW2.1 | 2 | 9429264-21032980 | 40.42-90.16 | 5.28 | |
qTGW5.1 | 5 | 6526040-6673100 | 27.98-28.61 | 2.11 |
表2 水稻穗部性状QTL分析
Table 2 QTL analysis of rice panicle traits
Traits | QTL | Chromosome | Physical distance (bp) | Position of support (cM) | Limit of detection (LOD) |
---|---|---|---|---|---|
Panicle length | qPL3.1 | 3 | 4514694-9883047 | 19.35-42.37 | 5.45 |
qPL5.1 | 5 | 16473540-17216667 | 70.62-73.80 | 2.54 | |
qPL11.1 | 11 | 17453046-17570705 | 74.82-75.32 | 2.29 | |
qPL11.2 | 11 | 26791773-26969035 | 114.85-115.61 | 2.61 | |
Grain number per panicle | qGNPP3.1 | 3 | 6470188-15088477 | 27.74-64.68 | 2.21 |
qGNPP5.1 | 5 | 16020128-18858682 | 68.67-80.84 | 2.36 | |
qGNPP5.2 | 5 | 27007193-27230204 | 115.77-116.73 | 2.41 | |
Seed-setting rate | qSSR1.1 | 1 | 7203302-7421463 | 30.88-31.81 | 2.41 |
qSSR2.1 | 2 | 30004559-34415035 | 128.62-147.53 | 3.47 | |
qSSR10.1 | 10 | 5567413-15675357 | 23.87-67.20 | 2.78 | |
Stigma exsertion rate | qSER5.1 | 5 | 926888-4584013 | 3.97-19.65 | 3.25 |
qSER10.1 | 10 | 10115736-11156109 | 43.36-47.82 | 2.41 | |
Number of primary branches | qNPB1.1 | 1 | 25791086-25812159 | 110.56-110.65 | 2.24 |
qNPB5.1 | 5 | 5350624-6230055 | 22.94-26.71 | 2.66 | |
Number of secondary branches | qNSB2.1 | 2 | 34269184-34415035 | 146.90-147.16 | 2.25 |
qNSB3.1 | 3 | 5859763-7034710 | 25.00-30.16 | 3.18 | |
qNSB5.1 | 5 | 14979844-15200902 | 64.21-65.16 | 2.13 | |
qNSB5.2 | 5 | 15944247-17819466 | 68.35-76.39 | 3.06 | |
qNSB6.1 | 6 | 4263281-5187517 | 18.28-22.24 | 2.88 | |
qNSB11.1 | 11 | 4416803-4813122 | 18.93-20.63 | 2.47 | |
Grain length | qGL3.1 | 3 | 24904943-26519283 | 106.76-113.68 | 2.26 |
qGL6.1 | 6 | 23905310-24717124 | 102.48-105.96 | 2.14 | |
Grain width | qGW2.1 | 2 | 7512377-21714903 | 32.20-93.09 | 3.61 |
qGW3.1 | 3 | 10039327-11005670 | 43.04-47.18 | 2.24 | |
qGW4.1 | 4 | 19218278-19642144 | 82.38-84.20 | 3.28 | |
qGW5.1 | 5 | 3697712-6833061 | 15.85-29.29 | 3.46 | |
qGW5.2 | 5 | 28202582-29177297 | 120.90-125.16 | 3.27 | |
qGW6.1 | 6 | 4227455-6069227 | 18.12-26.02 | 3.77 | |
Thousand grain weight | qTGW1.1 | 1 | 6064691-6235269 | 26.00-26.73 | 2.80 |
qTGW2.1 | 2 | 9429264-21032980 | 40.42-90.16 | 5.28 | |
qTGW5.1 | 5 | 6526040-6673100 | 27.98-28.61 | 2.11 |
Traits | Gene ID | Function |
---|---|---|
Panicle length | LOC_Os03g08900 | Multi antimicrobial extrusion protein |
LOC_Os03g10210 | Helix-turn-helix motif | |
LOC_Os03g10520 | Radical SAM enzyme | |
LOC_Os03g10620 | Strigolactone receptor | |
LOC_Os03g11670 | PPR domain containing protein | |
LOC_Os03g12120 | NAC domain | |
Grain number per panicle | LOC_Os05g30890 | CYP450 |
LOC_Os05g31040 | Cytokinin oxidase/dehydrogenase | |
LOC_Os05g31280 | Gibberellin regulated protein | |
LOC_Os05g31380 | Transcription factor GRAS domain containing protein | |
LOC_Os05g31740 | Cytochrome P450 86A2 | |
Seed-setting rate | LOC_Os02g50240 | Glutamine synthetase |
LOC_Os02g50740 | WD40 repeat containing protein | |
LOC_Os02g52990 | Auxin responsive SAUR protein family protein | |
LOC_Os02g53680 | Replication protein A | |
LOC_Os02g54910 | WD40 repeat containing protein | |
LOC_Os02g56140 | MYC-type, bHLH domain | |
Stigma exsertion rate | LOC_Os05g02870 | Similar to stigma/style ABC transporter |
LOC_Os05g04160 | Pentatricopeptide containing protein | |
LOC_Os05g04770 | F-box domain, cyclin-like domain containing protein | |
LOC_Os05g05280 | RING-type E3 ubiquitin ligase | |
LOC_Os05g05490 | Pentatricopeptide | |
LOC_Os05g06150 | Pentatricopeptide repeat domain containing protein | |
LOC_Os05g08350 | F-box domain, cyclin-like domain containing protein | |
Number of primary branches | LOC_Os05g09740 | HAD superfamily phosphatase |
LOC_Os05g10210 | HAD superfamily phosphatase | |
LOC_Os05g10370 | Acid phosphatase | |
LOC_Os05g10690 | Similar to transcription factor MYBS2 | |
LOC_Os05g10770 | Histone H3K4 demethylase | |
Number of secondary branches | LOC_Os03g11614 | MADS-domain transcription factor |
LOC_Os03g11700 | PPR repeat containing protein | |
LOC_Os03g11790 | Cyclin-like F-box domain containing protein | |
LOC_Os03g12430 | PPR repeat containing protein | |
LOC_Os03g12940 | Cyclin-like F-box domain containing protein | |
LOC_Os05g28720 | Pentatricopeptide repeat domain containing protein | |
Thousand grain weight | LOC_Os02g17880 | Glycosyl hydrolases family 16 |
LOC_Os02g18820 | WD40/YVTN repeat-like domain containing protein | |
LOC_Os02g19820 | RAG1-activating protein 1 homologue domain containing protein | |
LOC_Os02g29530 | Glycosyl transferase family 8 | |
LOC_Os02g30850 | CC-type glutaredoxin | |
LOC_Os02g32370 | Inositol polyphosphate kinase | |
LOC_Os05g11700 | Pentatrico peptide repeat (PPR) protein | |
Grain length | LOC_Os03g44430 | Ubiquitin carboxy-terminal hydrolase family 1 |
LOC_Os03g44500 | Ser/Thr phosphatase; cryptic inhibitor of cytokinin phosphorelay | |
LOC_Os03g45830 | Auxin responsive SAUR protein domain containing protein | |
LOC_Os03g46180 | Plant lipid transfer protein/seed storage/trypsin-alpha amylase inhibitor domain containing protein | |
Grain length | LOC_Os03g46560 | RPD1 |
Grain width | LOC_Os02g30910 | Sugar transporter |
LOC_Os06g10950 | Similar to glycosyltransferase family 37 | |
LOC_Os06g10960 | Similar to glycosyltransferase family 37 | |
LOC_Os06g11270 | UDP-glucuronosyl | |
LOC_Os06g09270 | Hypro1 |
表3 水稻穗部性状相关候选基因的功能
Table 3 The functions of candidate genes associated with rice panicle traits
Traits | Gene ID | Function |
---|---|---|
Panicle length | LOC_Os03g08900 | Multi antimicrobial extrusion protein |
LOC_Os03g10210 | Helix-turn-helix motif | |
LOC_Os03g10520 | Radical SAM enzyme | |
LOC_Os03g10620 | Strigolactone receptor | |
LOC_Os03g11670 | PPR domain containing protein | |
LOC_Os03g12120 | NAC domain | |
Grain number per panicle | LOC_Os05g30890 | CYP450 |
LOC_Os05g31040 | Cytokinin oxidase/dehydrogenase | |
LOC_Os05g31280 | Gibberellin regulated protein | |
LOC_Os05g31380 | Transcription factor GRAS domain containing protein | |
LOC_Os05g31740 | Cytochrome P450 86A2 | |
Seed-setting rate | LOC_Os02g50240 | Glutamine synthetase |
LOC_Os02g50740 | WD40 repeat containing protein | |
LOC_Os02g52990 | Auxin responsive SAUR protein family protein | |
LOC_Os02g53680 | Replication protein A | |
LOC_Os02g54910 | WD40 repeat containing protein | |
LOC_Os02g56140 | MYC-type, bHLH domain | |
Stigma exsertion rate | LOC_Os05g02870 | Similar to stigma/style ABC transporter |
LOC_Os05g04160 | Pentatricopeptide containing protein | |
LOC_Os05g04770 | F-box domain, cyclin-like domain containing protein | |
LOC_Os05g05280 | RING-type E3 ubiquitin ligase | |
LOC_Os05g05490 | Pentatricopeptide | |
LOC_Os05g06150 | Pentatricopeptide repeat domain containing protein | |
LOC_Os05g08350 | F-box domain, cyclin-like domain containing protein | |
Number of primary branches | LOC_Os05g09740 | HAD superfamily phosphatase |
LOC_Os05g10210 | HAD superfamily phosphatase | |
LOC_Os05g10370 | Acid phosphatase | |
LOC_Os05g10690 | Similar to transcription factor MYBS2 | |
LOC_Os05g10770 | Histone H3K4 demethylase | |
Number of secondary branches | LOC_Os03g11614 | MADS-domain transcription factor |
LOC_Os03g11700 | PPR repeat containing protein | |
LOC_Os03g11790 | Cyclin-like F-box domain containing protein | |
LOC_Os03g12430 | PPR repeat containing protein | |
LOC_Os03g12940 | Cyclin-like F-box domain containing protein | |
LOC_Os05g28720 | Pentatricopeptide repeat domain containing protein | |
Thousand grain weight | LOC_Os02g17880 | Glycosyl hydrolases family 16 |
LOC_Os02g18820 | WD40/YVTN repeat-like domain containing protein | |
LOC_Os02g19820 | RAG1-activating protein 1 homologue domain containing protein | |
LOC_Os02g29530 | Glycosyl transferase family 8 | |
LOC_Os02g30850 | CC-type glutaredoxin | |
LOC_Os02g32370 | Inositol polyphosphate kinase | |
LOC_Os05g11700 | Pentatrico peptide repeat (PPR) protein | |
Grain length | LOC_Os03g44430 | Ubiquitin carboxy-terminal hydrolase family 1 |
LOC_Os03g44500 | Ser/Thr phosphatase; cryptic inhibitor of cytokinin phosphorelay | |
LOC_Os03g45830 | Auxin responsive SAUR protein domain containing protein | |
LOC_Os03g46180 | Plant lipid transfer protein/seed storage/trypsin-alpha amylase inhibitor domain containing protein | |
Grain length | LOC_Os03g46560 | RPD1 |
Grain width | LOC_Os02g30910 | Sugar transporter |
LOC_Os06g10950 | Similar to glycosyltransferase family 37 | |
LOC_Os06g10960 | Similar to glycosyltransferase family 37 | |
LOC_Os06g11270 | UDP-glucuronosyl | |
LOC_Os06g09270 | Hypro1 |
图4 水稻穗部性状候选基因表达差异 (A) 穗长; (B) 每穗粒数; (C) 结实率; (D) 柱头外露率; (E) 一次枝梗数; (F) 二次枝梗数; (G) 千粒重; (H) 粒长; (I) 粒宽。* P<0.05; ** P<0.01
Figure 4 Differences in the expression of candidate genes for panicle traits in rice (A) Panicle length; (B) Grain number per panicle; (C) Seed-setting rate; (D) Stigma exsertion rate; (E) Number of primary branches; (F) Number of secondary branches; (G) Thousand grain weight; (H) Grain length; (I) Grain width. * P<0.05; ** P<0.01
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