水稻抗细菌性条斑病基因挖掘与初定位

doi:10.11983/CBB23071

植物学报 ›› 2024, Vol. 59 ›› Issue (1): 1-9.DOI: 10.11983/CBB23071

• 研究报告 • 下一篇

水稻抗细菌性条斑病基因挖掘与初定位

方妍力¹^,²^,^†, 田传玉¹^,²^,^†, 苏如意¹, 刘亚培², 王春连², 陈析丰¹, 郭威¹^,^*(), 纪志远²^,^*()

¹浙江师范大学生命科学学院, 金华 321004
²中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程, 北京 100081

收稿日期:2023-05-31 接受日期:2023-12-19 出版日期:2024-01-01 发布日期:2024-01-12
通讯作者: *E-mail: weiguo817@zjnu.cn; jizhiyuan@caas.cn
作者简介:
†共同第一作者
基金资助:
国家重点基础研究发展计划(2021YFD1200501);国家自然科学基金(32072412);科技创新2030-重大项目(2022ZD-0400203-2)

Mining and Preliminary Mapping of Rice Resistance Genes Against Bacterial Leaf Streak

Yanli Fang¹^,²^,^†, Chuanyu Tian¹^,²^,^†, Ruyi Su¹, Yapei Liu², Chunlian Wang², Xifeng Chen¹, Wei Guo¹^,^*(), Zhiyuan Ji²^,^*()

¹College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China
²National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2023-05-31 Accepted:2023-12-19 Online:2024-01-01 Published:2024-01-12
Contact: *E-mail: weiguo817@zjnu.cn; jizhiyuan@caas.cn
About author:
†These authors contributed equally to this paper

摘要/Abstract

摘要： 由稻黄单胞菌稻生致病变种(Xanthomonas oryzae pv. oryzicola, Xoc)引起的细菌性条斑病(BLS)是水稻(Oryza sativa)生产上的重要病害, 近年来其发病呈快速上升趋势, 特别是在我国南方(包括江苏、浙江、福建和广东等地)稻区危害严重。种植抗病品种是防治BLS最理想的措施, 但目前生产上严重缺乏可用于育种的优异抗病基因资源。通过人工接种鉴定筛选水稻种质资源, 挖掘到2份高抗BLS材料(M1和D1)。多菌株系接种结果表明, M1具有非小种特异性广谱抗病(RNS BSR)特征。经遗传群体分析表明, 栽培稻M1携带单个显性抗BLS新基因Xo-3。通过混池测序和关联分析, 将Xo-3基因初步定位在2号染色体上的一段候选区域内。抗BLS种质资源的挖掘及其抗性遗传基础的解析, 将有助于理解水稻-Xoc互作机理, 从而培育抗BLS水稻新品种和制定科学的防治BLS策略。

关键词: 细菌性条斑病, 种质资源, 抗病基因, 水稻, 稻黄单胞菌

Abstract: Bacterial leaf streak (BLS), caused by Xanthomonas oryzae pv. oryzicola (Xoc), is one of the most important diseases of rice, which is now highly prevalent in rice-growing regions of China, especially in Southern China (including Jiangsu, Zhejiang, Fujian, and Guangdong). Planting resistant varieties is considered as the best approach to control BLS. However, no resistant cultivars are available owing to limited genetic resources for BLS resistance. Two highly BLS-resistant materials (M1 and D1) have been discovered in our research by screening germplasm against BLS via syringe inoculation. Multi-strain inoculation showed that M1 had the characteristics of race-nonspecific broad-spectrum resistance (RNS BSR). The genetic population analysis showed that the cultivated rice M1 harbored a single dominant new gene Xo-3 of resistance to BLS. Through BSA-seq and association analysis, Xo-3 was initially mapped in a candidate region on chromosome 2. The mining of germplasm resources of BLS-resistant and the analysis of the genetic basis of its resistance will help our understanding of the interaction mechanism between rice and Xoc, so as to cultivate new varieties of BLS-resistant rice and development scientific strategies in controlling BLS.

Key words: bacterial leaf streak, germplasm resources, resistance gene, rice, Xanthomonas oryzae

方妍力, 田传玉, 苏如意, 刘亚培, 王春连, 陈析丰, 郭威, 纪志远. 水稻抗细菌性条斑病基因挖掘与初定位. 植物学报, 2024, 59(1): 1-9.

Yanli Fang, Chuanyu Tian, Ruyi Su, Yapei Liu, Chunlian Wang, Xifeng Chen, Wei Guo, Zhiyuan Ji. Mining and Preliminary Mapping of Rice Resistance Genes Against Bacterial Leaf Streak. Chinese Bulletin of Botany, 2024, 59(1): 1-9.

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图/表 6

图1 水稻细菌性条斑病(BLS)抗性种质鉴定示意图 (A) 水稻细菌性条斑病发生田块(bar=1 cm); (B) 注射接种; (C) 水稻抗性种质材料鉴定

Figure 1 Diagram for the identification of rice germplasms that confer resistance to bacterial leaf streak (BLS) (A) Occurrence of bacterial leaf streak in paddy (bar=1 cm); (B) Syringe inoculation; (C) Rice materials for resistance identification

图2 水稻细菌性条斑病(BLS)抗性的无针注射接种鉴定 (A) 代表性材料病斑类型; (B) 代表性材料病斑长度(柱形图上方不同小写字母表示差异极显著(P<0.01)); (C) 测试材料的抗性类型分布(R: 高抗; MR: 中抗; S: 感病; HS: 高感)

Figure 2 Identification of rice germplasms that confer resistance to bacterial leaf streak (BLS) with needleless syringes (A) Phenotype of representative rice materials; (B) Lesion length of representative rice materials (different lowercase letters above horizontal columns indicate significant statistical differences at P<0.01); (C) Distribution of rice materials in BLS disease reactions (R: High resistance; MR: Middle resistance; S: Susceptibility; HS: High susceptibility)

图3 M1和D1材料的白叶枯病(BB)抗性鉴定 (A) M1和D1接种白叶枯病菌(PXO99A)后的表型(bar=1 cm); (B) M1和D1的Xa5/xa5位点序列(红色虚线框表示变异位点)

Figure 3 Identification of M1 and D1 that confer resistance to bacterial blight (BB) (A) Disease response of M1 and D1 inoculated with Xoo (PXO99A) (bar=1 cm); (B) Sequence of Xa5/xa5 locus in M1 and D1 (the red dashed box represents the mutation sites)

图4 M1的抗细菌性条斑病(BLS)病谱鉴定分析 (A) Xoc强毒菌株接种Nip和M1后的表型(bar=0.5 cm); (B) 病斑长度(**表示差异极显著(P<0.01))

Figure 4 Bacterial leaf streak (BLS) resistance spectrum assay of M1 (A) Phenotype of hypervirulent Xoc strains in Nip and M1 (bar=0.5 cm); (B) Lesion length of hypervirulent Xoc strains in Nip and M1 (** indicate extremely significant differences (P<0.01))

图5 M1材料的细菌性条斑病(BLS)抗性遗传分析 (A) Nipponbare × M1群体F2单株的BLS抗病反应(bar=0.5 cm); (B) F2群体抗感单株的统计分析。R、MR、S和HS同图2。

Figure 5 Genetic analysis of M1 that confers resistance to bacterial leaf streak (BLS) (A) Resistance identification of F2 plants derived from the cross between Nipponbare and M1 (bar=0.5 cm); (B) Statistical analysis of resistant and susceptible F2 plants. R, MR, S, and HS are the same as shown in Figure 2.

图6 M1携带抗细菌性条斑病(BLS)基因Xo-3的BSA-Seq定位分析红色虚线框表示候选基因区间。

Figure 6 Mapping of bacterial leaf streak (BLS) resistance gene Xo-3 in M1 using BSA-Seq method The red dashed box represents the candidate gene region.

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水稻抗细菌性条斑病基因挖掘与初定位

Mining and Preliminary Mapping of Rice Resistance Genes Against Bacterial Leaf Streak

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