植物学报 ›› 2024, Vol. 59 ›› Issue (4): 574-584.DOI: 10.11983/CBB24039  cstr: 32102.14.CBB24039

• 研究报告 • 上一篇    下一篇

水稻突变体pe-1对弱光胁迫的响应机制

黄佳慧, 杨惠敏, 陈欣雨, 朱超宇, 江亚楠, 胡程翔, 连锦瑾, 芦涛, 路梅, 张维林*(), 饶玉春*()   

  1. 浙江师范大学生命科学学院, 金华 321004
  • 收稿日期:2024-03-11 接受日期:2024-05-07 出版日期:2024-07-10 发布日期:2024-07-10
  • 通讯作者: *E-mail: wlzhangwhu@163.com; 饶玉春, 博士/博士后, 教授, 博士生导师, “双龙学者”特聘教授, 浙江省“高校领军人才培养计划”高层次拔尖人才(2022年), 金华市青年拔尖人才(2023年), 浙江省发明协会理事、植物遗传专业委员会副主任, 中国作物学会会员, 浙江省遗传学会会员。主要从事水稻等禾谷类作物的分子遗传学研究。以第一作者或通讯作者身份在Science Bulletin和New Phytologist等权威期刊上发表论文80余篇,以合作者身份在Nature Plants、Proceedings of the National Academy of Sciences of the United States of America等杂志上发表论文30余篇。主持转基因国家重大专项子课题、国家自然科学基金、浙江省自然科学基金等科研项目。授权国家发明专利25项(第一完成人)。E-mail: ryc@zjnu.cn
  • 基金资助:
    浙江省自然科学基金重点项目(LZ23C130003);2024年国家级大学生创新创业训练计划和2024年浙江省大学生科技创新活动计划暨新苗人才计划

Response Mechanism of Rice Mutant pe-1 to Low Light Stress

Jiahui Huang, Huimin Yang, Xinyu Chen, Chaoyu Zhu, Yanan Jiang, Chengxiang Hu, Jinjin Lian, Tao Lu, Mei Lu, Weilin Zhang*(), Yuchun Rao*()   

  1. College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China
  • Received:2024-03-11 Accepted:2024-05-07 Online:2024-07-10 Published:2024-07-10
  • Contact: *E-mail: wlzhangwhu@163.com; ryc@zjnu.cn

摘要: 以γ射线诱变籼稻双科早(Oryza sativa subsp. indica cv. ‘Shuangkezao’)获得的早熟鲜绿突变体pe-1为实验材料, 在三叶期和分蘖期进行弱光胁迫, 探讨pe-1与野生型在形态特征、非生物胁迫相关酶活性及其调控基因表达量、叶绿素含量、叶绿体合成与降解及光形态建成相关基因表达对弱光响应的差异。结果表明, 与野生型相比, 弱光胁迫后, pe-1叶片黄化程度显著降低, 株高和叶面积显著增加; 三叶期和分蘖期的叶片中不同叶绿素含量变化不同。此外, pe-1叶绿素含量增加, 且其抗氧化应激反应相关酶过氧化氢酶(CAT)和过氧化物酶(POD)的活性及相关基因的表达量均高于野生型, 表明在弱光胁迫下pe-1活性氧清除能力增强, 适应能力更强。pe-1的光形态建成相关基因表达量高于野生型, 表明弱光处理下pe-1的光接收能力更强。综上, pe-1突变体具有抵御弱光胁迫的潜力, 该结果有助于耐弱光水稻品种的选育。

关键词: 水稻, pe-1, 弱光胁迫, 光形态建成, 叶绿素, 叶绿体合成与降解

Abstract: This study utilized the γ-ray-induced early-maturation, fresh-green mutant line pe-1 from indica rice as an experimental material. At the trilobal stage and the tillering stage, we observed differences in morphological characteristics between pe-1 and wild type. In addition, we measured the activity of antioxidant-related enzymes and their regulatory genes expression, chlorophyll content and chloroplast synthesis and degradation-related gene expression, and photomorphogenesis-related gene expression to detect the differences in the low light response between the pe-1 and wild type. The results showed that pe-1 exhibited less leaf yellowing, taller stature, and larger leaf area compared to wild type post-stress. The changes in chlorophyll content differed between leaves at the trilobal stage and the tillering stage. Additionally, pe-1 resulted in increased chlorophyll content and elevated levels of the stress-responsive enzymes catalase and peroxidase, as well as increased expression of related genes. This indicates enhanced reactive oxygen species sca- venging and stronger adaptability to adverse conditions under low light conditions. Moreover, pe-1 exhibited increased expression levels of genes associated with photomorphogenesis, indicating superior light perception ability under low light intensities. In summary, the pe-1 mutant shows immense potential for survival under low light stress, contributing to the breeding rice with low light tolerance.

Key words: rice, pe-1, low light stress, photomorphogenesis, chlorophyll, chloroplast synthesis and degradation