Chinese Bulletin of Botany ›› 2016, Vol. 51 ›› Issue (1): 89-97.DOI: 10.11983/CBB14207

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Callus Induction and High Efficiency Plant Regeneration System Establishment of Pararuellia delavayana

Meiping Lü1, Yuanzhong Wang2, Hengyu Huang1*   

  1. 1Engineering Research Center for Reproducing Fine Varieties of Chinese Medicinal Plants, Yunnan University of Chinese Traditional Medicine, Kunming 650500, China
    2Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China
  • Received:2014-12-08 Accepted:2015-05-30 Online:2016-01-01 Published:2016-02-01
  • Contact: Huang Hengyu
  • About author:? These authors contributed equally to this paper


The effectiveness of different combinations of growth regulators and concentrations on callus induction, bud induction and plant regeneration were studied using explants excised from seedlings of Pararuellia delavayana. The optimum explants for callus induction and plant regeneration were stem explants with nodes. The percentage of callus induction was up to 85.38% when stem explants with nodes were cultured on MS medium containing 1.0 mg·L-1 6-BA, 0.5 mg·L-1 NAA and 0.1 mg·L-1 KT after 17 days, The percentage of bud formation from callus was up to 97.55% after 25 days, and the frequency of adventitious bud formation was 15.38 after 30 days. Vitrification emerged after 6 subculture cycles, and the phenomenon was aggravated with increasing generation, but the proliferation rate decreased. MS and B5 media were used interchangeably to solve the problem. The optimal medium for rooting was MS+0.5 mg·L-1 NAA; the rooting rate was 100% and the survival rate of transplants was >95%. We established a rapid propagation system and provided an effective solution for protecting wild resources and sprout multiplication of P. delavayana, while providing an experimental base for research in genetic transformation.

Key words: Pararuellia delavayana, stem with nodes, callus, vitrification, optimal culture