多肉植物劳尔的组织培养

doi:10.11983/CBB15036

植物学报 ›› 2016, Vol. 51 ›› Issue (2): 251-256.DOI: 10.11983/CBB15036 cstr: 32102.14.CBB15036

多肉植物劳尔的组织培养

刘芳¹^,^†, 唐映红¹^,²^,^†, 袁有美², 郭清泉¹, 沈帆¹, 陈建荣¹^,^*()

¹长沙学院生物与环境工程系, 长沙 410022
²湖南农业大学农学院, 长沙 410128

收稿日期:2015-02-16 接受日期:2015-05-11 出版日期:2016-03-01 发布日期:2016-03-31
通讯作者: *E-mail: 249809957@qq.com
作者简介:第一联系人：
^†共同第一作者
基金资助:
湖南省科技计划(2014CK4018)

Tissue Culture of the Succulent Plant Sedum clavatum

Fang Liu¹^,^†, Yinghong Tang¹^,²^,^†, Youmei Yuan², Qingquan Guo¹, Fan Shen¹, Jianrong Chen¹^,^*()

¹Department of Biological and Environmental Engineering of Changsha University, Changsha 410022, China
²Agriculture College of Hunan Agriculture University, Changsha 410128, China

Received:2015-02-16 Accepted:2015-05-11 Online:2016-03-01 Published:2016-03-31
Contact: *E-mail: 249809957@qq.com
About author:First author contact:
^†These authors contributed equally to this paper

摘要/Abstract

摘要： 以多肉植物劳尔(Sedum clavatum)叶片为外植体, 通过诱导愈伤组织、愈伤组织分化新芽、芽生根和无菌苗移栽等步骤建立劳尔无菌苗快速繁殖体系。研究结果表明, 诱导劳尔叶片愈伤组织的最佳培养基为MS+3.0 mg·L^-1 6-BA+0.1 mg·L^-1 NAA+1 mg·L^-1 KT, 诱导率达95.7%; 愈伤组织诱导分化新芽的最佳培养基为3/4MS+3.0 mg·L^-1 6-BA+0.3 mg·L^-1 NAA, 分化率达80%; 芽生根的最佳培养基为1/2MS+0.03 mg·L^-1 NAA, 生根率可达94.89%; 采用珍珠岩:蛭石(1:2)作为基质移栽培养生根苗, 成活率达90%以上。实验成功建立了劳尔快速繁殖体系。

关键词: 劳尔, 愈伤组织诱导, 植株再生

Abstract: The leaf of Sedum clavatum was used to induce callus; through callus induction, bud induction, rooting and transplatation, aseptic-frequency regeneration systems were established. The optimal medium for induction was MS+3.0 mg·L^-1 6-BA+0.1 mg·L^-1NAA+1.0 mg·L^-1 KT; for redifferentiation 3/4MS+3.0 mg·L^-1 6-BA+0.3mg·L^-1 NAA; and for rooting 1/2MS+0.03 mg·L^-1 NAA. The callus induction, redifferentiation and rooting rate was 95.7%, 80% and 94.89%, respectively. Using perlite:vermiculite (1:2) as a matrix, the transplant survival rate was >90%. We have established a high-frequency regeneration system for S. clavatum.

Key words: Sedum clavatum, callus induction, plant regeneration

中图分类号:

Q945.52

刘芳, 唐映红, 袁有美, 郭清泉, 沈帆, 陈建荣. 多肉植物劳尔的组织培养. 植物学报, 2016, 51(2): 251-256.

Fang Liu, Yinghong Tang, Youmei Yuan, Qingquan Guo, Fan Shen, Jianrong Chen. Tissue Culture of the Succulent Plant Sedum clavatum. Chinese Bulletin of Botany, 2016, 51(2): 251-256.

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https://www.chinbullbotany.com/CN/Y2016/V51/I2/251

图/表 8

参考文献 15

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Level	Factor (mg·L^-1)
Level	6-BA (A)	NAA (B)	KT (C)
1	0.5	0	0
2	1.5	0.1	0.5
3	3.0	0.2	1.0
4	4.5	0.3	1.5

Level	Factor (mg·L^-1)
Level	6-BA (A)	NAA (B)	KT (C)
1	0.5	0	0
2	1.5	0.1	0.5
3	3.0	0.2	1.0
4	4.5	0.3	1.5

No.	Concentrations of plant growth regulator (mg·L^-1)			Callus induction (%)	State of growth
No.	6-BA (A)	NAA (B)	KT (C)	Callus induction (%)	State of growth
1	0.5	0	0	20.0	Less calli could be induced; calli grew slowly and became yellow; lumps were small
2	0.5	0.1	0.5	32.5	Less calli could be induced; calli grew slowly and became yellow; lumps were small
3	0.5	0.2	1.0	47	Less calli could be induced; calli grew slowly and became light green; lumps were small
4	0.5	0.3	1.5	32.3	Less calli could be induced; calli grew slowly and became yellow; lumps were small
5	1.5	0	0.5	71.8	Less calli could be induced; calli grew quickly and became light green; lumps were small
6	1.5	0.1	0	74.9	Less calli could be induced; calli grew quickly and became light green; lumps were small
7	1.5	0.2	1.5	76.7	Less calli could be induced; calli grew quickly and became light green; lumps were small
8	1.5	0.3	1.0	74.1	Less calli could be induced; calli grew quickly and became light green; lumps were small
9	3	0	1.0	83.9	More calli could be induced; the way of callus induction is transverse expansion; calli grew quickly and became bright green; lumps were larger
10	3	0.1	1.5	95.7	More calli could be induced; the way of callus induction is transverse expansion; calli grew quickly and became bright green; lumps were larger
11	3	0.2	0	85.6	More calli could be induced; the way of callus induction is transverse expansion; calli grew quickly and became bright green; lumps were larger
12	3	0.3	0.5	81	More calli could be induced; the way of callus induction is transverse expansion; calli grew quickly and became bright green; lumps were larger
13	4.5	0	1.5	82.1	More calli could be induced; the way of callus induction is longitudinal expansion; calli grew quickly and became yellow green; lumps were larger
14	4.5	0.1	1.0	83.3	More calli could be induced; the way of callus induction is longitudinal expansion; calli grew quickly and became yellow green; lumps were larger
15	4.5	0.2	0.5	70.0	Less calli could be induced; calli could go into medium; calli grew quickly and became fuchsia; lumps were small
16	4.5	0.3	0	55.0	Less calli could be induced; calli could go into medium; calli grew quickly and became fuchsia; lumps were small

No.	Concentrations of plant growth regulator (mg·L^-1)			Callus induction (%)	State of growth
No.	6-BA (A)	NAA (B)	KT (C)	Callus induction (%)	State of growth
1	0.5	0	0	20.0	Less calli could be induced; calli grew slowly and became yellow; lumps were small
2	0.5	0.1	0.5	32.5	Less calli could be induced; calli grew slowly and became yellow; lumps were small
3	0.5	0.2	1.0	47	Less calli could be induced; calli grew slowly and became light green; lumps were small
4	0.5	0.3	1.5	32.3	Less calli could be induced; calli grew slowly and became yellow; lumps were small
5	1.5	0	0.5	71.8	Less calli could be induced; calli grew quickly and became light green; lumps were small
6	1.5	0.1	0	74.9	Less calli could be induced; calli grew quickly and became light green; lumps were small
7	1.5	0.2	1.5	76.7	Less calli could be induced; calli grew quickly and became light green; lumps were small
8	1.5	0.3	1.0	74.1	Less calli could be induced; calli grew quickly and became light green; lumps were small
9	3	0	1.0	83.9	More calli could be induced; the way of callus induction is transverse expansion; calli grew quickly and became bright green; lumps were larger
10	3	0.1	1.5	95.7	More calli could be induced; the way of callus induction is transverse expansion; calli grew quickly and became bright green; lumps were larger
11	3	0.2	0	85.6	More calli could be induced; the way of callus induction is transverse expansion; calli grew quickly and became bright green; lumps were larger
12	3	0.3	0.5	81	More calli could be induced; the way of callus induction is transverse expansion; calli grew quickly and became bright green; lumps were larger
13	4.5	0	1.5	82.1	More calli could be induced; the way of callus induction is longitudinal expansion; calli grew quickly and became yellow green; lumps were larger
14	4.5	0.1	1.0	83.3	More calli could be induced; the way of callus induction is longitudinal expansion; calli grew quickly and became yellow green; lumps were larger
15	4.5	0.2	0.5	70.0	Less calli could be induced; calli could go into medium; calli grew quickly and became fuchsia; lumps were small
16	4.5	0.3	0	55.0	Less calli could be induced; calli could go into medium; calli grew quickly and became fuchsia; lumps were small

K	6-BA (A)	NAA (B)	KT (C)
K₁	32.95	64.45	58.88
K₂	74.38	71.60	63.83
K₃	88.55	69.83	72.08
K₄	72.6	60.60	71.70
R	53.6	11.00	13.20
The optimum level	A₃	B₂	C₃
The optimum assembly	A₃B₂C₃

多肉植物劳尔的组织培养

Tissue Culture of the Succulent Plant Sedum clavatum

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Element	DEVSQ	Degree of freedom	F	Significance
6-BA (A)	6507.10	3	201.38	*
NAA (B)	304.09	3	9.41	*
KT (C)	493.44	3	15.27	*

No.	Concentrations of plant growth regulator (mg·L^-1)		Differentiation rate (%)
No.	6-BA (D)	NAA (E)	Differentiation rate (%)
1	0	0	10.0
2	1	0	20.3
3	2	0	25.1
4	3	0	34.6
5	4	0	30.4
6	0	0.1	20.1
7	1	0.1	27.1
8	2	0.1	35.1
9	3	0.1	45.0
10	4	0.1	40.4
11	0	0.2	25.2
12	1	0.2	30.0
13	2	0.2	40.0
14	3	0.2	64.5
15	4	0.2	55.2
16	0	0.3	34.8
17	1	0.3	44.9
18	2	0.3	55.1
19	3	0.3	80.0
20	4	0.3	64.4
21	0	0.4	19.6
22	1	0.4	26.7
23	2	0.4	30.2
24	3	0.4	70.3
25	4	0.4	50.0

Sources of variation (mg·L^-1)	Sum of squares	F	Signifi- cance	Level	Differentiation rate (%)
6-BA (D)	4433.71	32.61	**	4	61.47 aA
				5	49.63 abAB
				3	40.83 bcBC
				2	30.68 cdCD
				1	23.37 dD
NAA (E)	2837.77	22.35	**	4	58.15 aA
				3	47.35 bAB
				5	39.78 bcB
				2	33.73 cdBC
				1	25.86 dC

Number	NAA (mg·L^-1)	Rooting rate (%)	The state of root	Growth performance
G1	0	0.00 dD	Rootless	Yellow leaves
G2	0.01	53.32 cC	Short and thin	Secretary out some white root points; green leaf
G3	0.02	68.67 bB	Short and thick	Secretary out some white root points; green leaf
G4	0.03	94.89 aA	Long and thick	More roots at base; green leaf
G5	0.04	59.53 cC	Short and thin	Information organization and a few root points at base; green leaf

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