植物学报 ›› 2019, Vol. 54 ›› Issue (6): 773-778.DOI: 10.11983/CBB18256
张文婷1,何燕红1,舒宁1,邢景景1,刘宝骏1,包满珠1,刘国锋2,*(
)
首页收稿日期:2018-11-29
接受日期:2019-03-05
出版日期:2019-11-01
发布日期:2020-07-09
通讯作者:
刘国锋
基金资助:
Wenting Zhang1,Yanhong He1,Ning Shu1,Jingjing Xing1,Baojun Liu1,Manzhu Bao1,Guofeng Liu2,*()
Received:2018-11-29
Accepted:2019-03-05
Online:2019-11-01
Published:2020-07-09
Contact:
Guofeng Liu
摘要： 以金黄花滇百合(Lilium bakerianum var. aureum)的鳞片为外植体, 探讨不同部位外植体和不同配比的植物生长调节剂对愈伤组织诱导和植株再生的影响。研究结果表明, 鳞片诱导愈伤组织和不定芽的最适培养基为MS+1.0 mg∙L -16-BA+0.1 mg∙L -1NAA+30 g∙L -1蔗糖; 不定芽增殖的最适培养基为MS+0.5 mg∙L -16-BA+0.1 mg∙L -1NAA+30 g∙L -1蔗糖; 不定芽诱导小鳞茎的最适培养基为MS+1.0 mg∙L -1NAA+30 g∙L -1蔗糖; 小鳞茎膨大生根的最适培养基为1/2MS+0.01 mg∙L -1NAA+60 g∙L -1蔗糖。该研究为金黄花滇百合种质资源保护和快速繁殖提供了技术支撑。
张文婷,何燕红,舒宁,邢景景,刘宝骏,包满珠,刘国锋. 金黄花滇百合植株再生与离体快繁技术体系的建立. 植物学报, 2019, 54(6): 773-778.
Wenting Zhang,Yanhong He,Ning Shu,Jingjing Xing,Baojun Liu,Manzhu Bao,Guofeng Liu. Plant Regeneration and Rapid Propagation System of Lilium bakerianum var. aureum. Chinese Bulletin of Botany, 2019, 54(6): 773-778.
| Treatments | Explants | 6-BA (mg∙L-1) | NAA (mg∙L-1) | No. of explants | Survival rate (%) | No. of induced buds | No. of induced callus |
|---|---|---|---|---|---|---|---|
| 1 | Bulbs | 0 | 0 | 8 | 100 | 0 | 0 |
| 2 | Bulbs | 0.5 | 0.5 | 15 | 80.0 | 3 | 11 |
| 3 | Scale | 0 | 0 | 30 | 10.0 | 3 | 0 |
| 4 | Scale | 0.5 | 0.1 | 10 | 30.0 | 3 | 0 |
| 5 | Scale | 0.5 | 0.5 | 30 | 0 | 0 | 0 |
| 6 | Scale | 2.0 | 0.5 | 30 | 23.3 | 2 | 7 |
| 7 | Scale | 1.5 | 0.5 | 10 | 20.0 | 2 | 0 |
| 8 | Callus | 0 | 0 | 13 | 23.1 | 2 | 13 (browning) |
| 9 | Callus | 0.5 | 0.1 | 30 | 83.3 | 53 | 30 (swelled obviously) |
| 10 | Callus | 0.5 | 0.5 | 24 | 62.5 | 46 | 24 (callus proliferation) |
| 11 | Callus | 2.0 | 0.5 | 15 | 80.0 | 14 | 15 (bulbs differentiation) |
| 12 | Callus | 1.0 | 0.1 | 22 | 81.8 | 71 | 22 (budding obviously) |
| 13 | Petiole | 0.5 | 0.1 | 118 | 5.9 | 0 | 7 |
| 14 | Petiole | 0.5 | 0.5 | 60 | 6.7 | 1 | 3 |
| 15 | Petiole | 2.0 | 0.5 | 60 | 0 | 0 | 0 |
| 16 | Petiole | 1.0 | 0.1 | 90 | 4.4 | 9 | 0 |
| 17 | Leaf | 0.5 | 0.1 | 60 | 0 | 0 | 0 |
| 18 | Leaf | 0.5 | 0.5 | 60 | 0 | 0 | 0 |
| 19 | Leaf | 2.0 | 0.5 | 60 | 0 | 0 | 0 |
表1 金黄花滇百合不同部位外植体再生能力比较
Table 1 Comparison of regeneration capabilities of different explants from Lilium bakerianum var. aureum
| Treatments | Explants | 6-BA (mg∙L-1) | NAA (mg∙L-1) | No. of explants | Survival rate (%) | No. of induced buds | No. of induced callus |
|---|---|---|---|---|---|---|---|
| 1 | Bulbs | 0 | 0 | 8 | 100 | 0 | 0 |
| 2 | Bulbs | 0.5 | 0.5 | 15 | 80.0 | 3 | 11 |
| 3 | Scale | 0 | 0 | 30 | 10.0 | 3 | 0 |
| 4 | Scale | 0.5 | 0.1 | 10 | 30.0 | 3 | 0 |
| 5 | Scale | 0.5 | 0.5 | 30 | 0 | 0 | 0 |
| 6 | Scale | 2.0 | 0.5 | 30 | 23.3 | 2 | 7 |
| 7 | Scale | 1.5 | 0.5 | 10 | 20.0 | 2 | 0 |
| 8 | Callus | 0 | 0 | 13 | 23.1 | 2 | 13 (browning) |
| 9 | Callus | 0.5 | 0.1 | 30 | 83.3 | 53 | 30 (swelled obviously) |
| 10 | Callus | 0.5 | 0.5 | 24 | 62.5 | 46 | 24 (callus proliferation) |
| 11 | Callus | 2.0 | 0.5 | 15 | 80.0 | 14 | 15 (bulbs differentiation) |
| 12 | Callus | 1.0 | 0.1 | 22 | 81.8 | 71 | 22 (budding obviously) |
| 13 | Petiole | 0.5 | 0.1 | 118 | 5.9 | 0 | 7 |
| 14 | Petiole | 0.5 | 0.5 | 60 | 6.7 | 1 | 3 |
| 15 | Petiole | 2.0 | 0.5 | 60 | 0 | 0 | 0 |
| 16 | Petiole | 1.0 | 0.1 | 90 | 4.4 | 9 | 0 |
| 17 | Leaf | 0.5 | 0.1 | 60 | 0 | 0 | 0 |
| 18 | Leaf | 0.5 | 0.5 | 60 | 0 | 0 | 0 |
| 19 | Leaf | 2.0 | 0.5 | 60 | 0 | 0 | 0 |
| Treatments | 6-BA (mg∙L-1) | NAA (mg∙L-1) | GA3 (mg∙L-1) | Induction ratio (%) |
|---|---|---|---|---|
| 1 | 0.5 | 0 | 0 | 23.3±21.00 b |
| 2 | 0.5 | 0.1 | 0 | 33.3±6.00 b |
| 3 | 1.0 | 0.1 | 0 | 50.00±10.00 a |
| 4 | 1.0 | 0.1 | 1.0 | 0.00±0.00 d |
| 5 | 1.5 | 0.5 | 0 | 6.70±6.00 cd |
表2 植物生长调节剂对金黄花滇百合鳞片诱导愈伤组织和不定芽的影响
Table 2 Effects of plant growth regulators on the induction of callus and adventitious buds of Lilium bakerianum var. aureum
| Treatments | 6-BA (mg∙L-1) | NAA (mg∙L-1) | GA3 (mg∙L-1) | Induction ratio (%) |
|---|---|---|---|---|
| 1 | 0.5 | 0 | 0 | 23.3±21.00 b |
| 2 | 0.5 | 0.1 | 0 | 33.3±6.00 b |
| 3 | 1.0 | 0.1 | 0 | 50.00±10.00 a |
| 4 | 1.0 | 0.1 | 1.0 | 0.00±0.00 d |
| 5 | 1.5 | 0.5 | 0 | 6.70±6.00 cd |
| Treatments | 6-BA (mg∙L-1) | NAA (mg∙L-1) | Proliferation multiple | Growth state |
|---|---|---|---|---|
| 1 | 0.5 | 0.1 | 3.87±0.24 a | Strong |
| 2 | 0.5 | 0.5 | 1.87±0.18 b | Sparse, slightly yellow |
| 3 | 1.0 | 0.1 | 1.06±0.17 c | Sparse, yellow |
| 4 | 1.0 | 0.5 | 1.23±0.09 c | Sparse, yellow |
| 5 | 2.0 | 0.5 | 0.93±0.24 c | Sparse, yellow |
表3 植物生长调节剂对金黄花滇百合不定芽增殖的影响
Table 3 Effects of plant growth regulators on the multiplication of adventitious buds of Lilium bakerianum var. aureum
| Treatments | 6-BA (mg∙L-1) | NAA (mg∙L-1) | Proliferation multiple | Growth state |
|---|---|---|---|---|
| 1 | 0.5 | 0.1 | 3.87±0.24 a | Strong |
| 2 | 0.5 | 0.5 | 1.87±0.18 b | Sparse, slightly yellow |
| 3 | 1.0 | 0.1 | 1.06±0.17 c | Sparse, yellow |
| 4 | 1.0 | 0.5 | 1.23±0.09 c | Sparse, yellow |
| 5 | 2.0 | 0.5 | 0.93±0.24 c | Sparse, yellow |
| Treatments | 6-BA (mg∙L-1) | NAA (mg∙L-1) | No. of induced bulbs | Growth state |
|---|---|---|---|---|
| 1 | 0 | 0.5 | 1.73±0.23 d | Sparse leaves, thick roots |
| 2 | 0 | 1.0 | 4.70±0.46 a | Sparse leaves, thick roots |
| 3 | 0 | 2.0 | 2.47±0.00 cd | Thick leaves, thick roots |
| 4 | 0.2 | 0.5 | 2.80±1.00 c | Yellow leaves, rootless |
| 5 | 0.2 | 1.0 | 2.03±0.32 d | Yellow leaves, rootless |
| 6 | 0.2 | 2.0 | 3.50±0.42 b | Strong leaves, thick roots |
表4 植物生长调节剂对金黄花滇百合不定芽诱导小鳞茎的影响
Table 4 Effects of plant growth regulators on the induction of bulb from adventitious buds of Lilium bakerianum var. aureum
| Treatments | 6-BA (mg∙L-1) | NAA (mg∙L-1) | No. of induced bulbs | Growth state |
|---|---|---|---|---|
| 1 | 0 | 0.5 | 1.73±0.23 d | Sparse leaves, thick roots |
| 2 | 0 | 1.0 | 4.70±0.46 a | Sparse leaves, thick roots |
| 3 | 0 | 2.0 | 2.47±0.00 cd | Thick leaves, thick roots |
| 4 | 0.2 | 0.5 | 2.80±1.00 c | Yellow leaves, rootless |
| 5 | 0.2 | 1.0 | 2.03±0.32 d | Yellow leaves, rootless |
| 6 | 0.2 | 2.0 | 3.50±0.42 b | Strong leaves, thick roots |
| Treatments | MS | Sucrose (g∙L-1) | NAA (mg∙L-1) | IBA (mg∙L-1) | Swell times | Growth state |
|---|---|---|---|---|---|---|
| 1 | 0.5 | 120 | 0.3 | 0.1 | 2.63±0.35 b | Withered leaves, thick roots |
| 2 | 0.5 | 90 | 0.3 | 0.1 | 1.94±0.41 c | Sparse leaves, thick roots |
| 3 | 0.5 | 60 | 0.3 | 0.1 | 0.57±0.29 d | Rootless |
| 4 | 0.5 | 120 | 0.3 | 0 | 1.57±0.06 c | Curly leaves, black roots |
| 5 | 0.5 | 90 | 0.3 | 0 | 1.58±0.14 c | Thick leaves, thin scales |
| 6 | 0.5 | 60 | 0.3 | 0 | 1.00±0.25 d | Thick leaves, thin roots |
| 7 | 0.5 | 60 | 0.01 | 0 | 2.78±0.19 ab | Thick leaves, thick roots |
| 8 | 0.5 | 60 | 0.1 | 0 | 2.67±0.33 ab | Sparse leaves, thin roots |
表5 植物生长调节剂对金黄花滇百合鳞茎膨大及生根的影响
Table 5 Effects of plant growth regulators on the bulb expansion and rooting of Lilium bakerianum var. aureum
| Treatments | MS | Sucrose (g∙L-1) | NAA (mg∙L-1) | IBA (mg∙L-1) | Swell times | Growth state |
|---|---|---|---|---|---|---|
| 1 | 0.5 | 120 | 0.3 | 0.1 | 2.63±0.35 b | Withered leaves, thick roots |
| 2 | 0.5 | 90 | 0.3 | 0.1 | 1.94±0.41 c | Sparse leaves, thick roots |
| 3 | 0.5 | 60 | 0.3 | 0.1 | 0.57±0.29 d | Rootless |
| 4 | 0.5 | 120 | 0.3 | 0 | 1.57±0.06 c | Curly leaves, black roots |
| 5 | 0.5 | 90 | 0.3 | 0 | 1.58±0.14 c | Thick leaves, thin scales |
| 6 | 0.5 | 60 | 0.3 | 0 | 1.00±0.25 d | Thick leaves, thin roots |
| 7 | 0.5 | 60 | 0.01 | 0 | 2.78±0.19 ab | Thick leaves, thick roots |
| 8 | 0.5 | 60 | 0.1 | 0 | 2.67±0.33 ab | Sparse leaves, thin roots |
图1 金黄花滇百合鳞片植株再生体系的建立 (A), (B) 鳞片诱导不定芽; (C) 愈伤组织分化不定芽; (D) 不定芽增殖; (E) 不定芽诱导小鳞茎; (F) 再生植株; (G) 植株生根; (H), (I) 移栽。Bars=1 cm
Figure 1 Establishment of plantlet regeneration from scales of Lilium bakerianum var. aureum (A), (B) Induction of new bud from scales; (C) Differentiation of new bud from callus; (D) The multiplication culture of adventitious buds; (E) The forming of bulb from adventitious bud; (F) Regenerated plantlets; (G) Plantlets rooting; (H), (I) Transplanting. Bars=1 cm
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