植物学报 ›› 2019, Vol. 54 ›› Issue (6): 773-778.DOI: 10.11983/CBB18256

• 技术方法 • 上一篇    下一篇

金黄花滇百合植株再生与离体快繁技术体系的建立

张文婷1,何燕红1,舒宁1,邢景景1,刘宝骏1,包满珠1,刘国锋2,*()   

  1. 1 华中农业大学园艺林学学院, 园艺植物生物学教育部重点实验室, 武汉 430070
    2 广州市林业和园林科学研究院, 广州 510405
  • 收稿日期:2018-11-29 接受日期:2019-03-05 出版日期:2019-11-01 发布日期:2020-07-09
  • 通讯作者: 刘国锋
  • 基金资助:
    公益性行业(农业)科研项目(No.201203071)

Plant Regeneration and Rapid Propagation System of Lilium bakerianum var. aureum

Wenting Zhang1,Yanhong He1,Ning Shu1,Jingjing Xing1,Baojun Liu1,Manzhu Bao1,Guofeng Liu2,*()   

  1. 1 Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China
    2 Guangzhou Institute of Forestry and Landscape Architecture, Guangzhou 510405, China
  • Received:2018-11-29 Accepted:2019-03-05 Online:2019-11-01 Published:2020-07-09
  • Contact: Guofeng Liu

摘要: 以金黄花滇百合(Lilium bakerianum var. aureum)的鳞片为外植体, 探讨不同部位外植体和不同配比的植物生长调节剂对愈伤组织诱导和植株再生的影响。研究结果表明, 鳞片诱导愈伤组织和不定芽的最适培养基为MS+1.0 mg∙L -16-BA+0.1 mg∙L -1NAA+30 g∙L -1蔗糖; 不定芽增殖的最适培养基为MS+0.5 mg∙L -16-BA+0.1 mg∙L -1NAA+30 g∙L -1蔗糖; 不定芽诱导小鳞茎的最适培养基为MS+1.0 mg∙L -1NAA+30 g∙L -1蔗糖; 小鳞茎膨大生根的最适培养基为1/2MS+0.01 mg∙L -1NAA+60 g∙L -1蔗糖。该研究为金黄花滇百合种质资源保护和快速繁殖提供了技术支撑。

关键词: 金黄花滇百合, 组织培养, 植株再生, 快速繁殖, 愈伤组织

Abstract: We investigated the effects of different explants, kinds and concentrations of plant growth regulators on callus induction and plant regeneration from scales of Lilium bakerianum var. aureum at tissue culture. The medium MS+1.0 mg∙L -16-BA+0.1 mg∙L -1NAA+30 g∙L -1 sucrose was suitable for inducing adventitious buds and calli. The medium MS+0.5 mg∙L -16-BA+0.1 mg∙L -1NAA+30 g∙L -1 sucrose was suitable for adventitious bud multiplication. MS+1.0 mg∙L -1NAA+30 g∙L -1 sucrose was optimal for bulblet formation from adventitious buds, and the optimal medium for the growth of bulblets was 1/2MS+0.01 mg∙L -1NAA+60 g∙L -1 sucrose. We provide technical support for germplasm resource protection and rapid propagation of L. bakerianum var. aureum.

Key words: Lilium bakerianum var. aureum, tissue culture, plant regeneration, rapid propagation, calli