关键词:

毛建草,   愈伤组织诱导,   分化,   增殖,   生根

Abstract: Dracocephalum rupestre field leaves and tissue-cultured seedling leaves were used as explants to investigate the impact of hormones on callus induction, differentiation, shoot proliferation, and rooting. The aim was to establish a leaf explant regeneration system. Results indicated that the optimal medium for inducing callus from field leaves was MS + 1 mg·L ⁻¹ 6-BA + 1 mg·L ⁻¹ 2,4-D + 0.1 mg·L ⁻¹ IAA, achieving an induction rate of 84.51%. For adventitious bud differentiation, the preferred medium comprised MS + 3 mg·L ⁻¹ 6-BA + 0.5 mg·L ⁻¹ TDZ + 0.5 mg·L ⁻¹ IAA, resulting in a differentiation rate of 66.37%. Similarly, for inducing callus from tissue-cultured seedling leaves, the optimal medium included MS + 2 mg·L ⁻¹ 6-BA + 0.1 mg·L ⁻¹ 2,4-D + 0.5 mg·L ⁻¹ IAA, with an induction rate of 86.73%. The medium conducive to adventitious bud differentiation consisted of MS + 2 mg·L ⁻¹ 6-BA + 2 mg·L ^-1 TDZ + 0.05 mg·L ⁻¹ IAA, yielding a differentiation rate of 53.48%. Furthermore, the appropriate medium for shoot proliferation was MS + 2 mg·L ⁻¹ 6-BA + 0.05 mg·L ⁻¹ NAA, achieving a proliferation rate of 83.57%. The rooting medium was 1/2MS + 0.1 mg·L ⁻¹ NAA + 0.1 mg·L ⁻¹ IBA, resulting in a rooting rate of 86.97%. Tissue-cultured seedlings exhibited optimal growth in a mixed substrate of peat and vermiculite at a ratio of 1:1 (v/v). This study successfully established a leaf explant regeneration system for D . rupestre, providing valuable technical support for the conservation and rapid propagation of its germplasm resources.

Key words: Dracocephalum rupestre, callus induction, differentiation, proliferation, rooting