植物学报

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毛建草愈伤组织诱导及植株再生

田旭平*, 岳康杰, 王佳丽, 刘慧欣, 史子尹, 亢红伟
  

  1. 山西农业大学林学院, 太谷 030801



  • 收稿日期:2023-12-29 修回日期:2024-05-06 出版日期:2024-05-16 发布日期:2024-05-16
  • 通讯作者: 田旭平
  • 基金资助:

    山西农业大学科技创新基金(No.2020BQ37)

Callus Induction and Plant Regeneration of Dracocephalum rupestre

Xuping Tian*, Kangjie Yue, Jiali Wang, Huixin Liu, Ziyin Shi, Hongwei Kang #br#   

  1. Forestry College of Shanxi Agricultural University, Taigu 030800, China


     

  • Received:2023-12-29 Revised:2024-05-06 Online:2024-05-16 Published:2024-05-16
  • Contact: Xuping Tian

摘要: 以毛建草(Dracocephalum rupestre)大田叶片和组培苗叶片为外植体, 探讨激素对愈伤组织诱导分化、不定芽增殖及生根的影响, 建立了叶片离体再生体系。结果表明, 诱导大田叶片愈伤组织的最适培养基MS+1.0 mg·L−1 6-BA+1.0 mg·L−1 2,4-D+0.1 mg·L−1 IAA, 诱导率达84.51%, 不定芽分化最佳培养基MS+3.0 mg·L−1 6-BA+0.5 mg·L−1 TDZ+0.5 mg·L−1 IAA, 分化率为66.37%; 诱导组培苗叶片愈伤组织的最适培养基为MS+2.0 mg·L−1 6-BA+0.1 mg·L−1 2,4-D+0.5 mg·L−1 IAA, 诱导率达86.73%, 不定芽分化最佳培养基MS+2.0 mg·L−1 6-BA+2.0 mg·L−1 TDZ+0.05 mg·L−1 IAA, 分化率为53.48%。适宜不定芽增殖培养基为MS+2 mg·L−1 6-BA+0.05 mg·L−1 NAA, 增殖率为83.57%, 最适生根培养基为1/2MS+0.1 mg·L−1 NAA+0.1 mg·L−1 IBA, 生根率为86.97%; 在草炭:蛭石=1:1 (v/v)的混合基质中组培苗长势最好。该研究建立了毛建草叶片离体培养再生体系, 毛建草种质资源保和种苗快繁提供了技术支持。

关键词:

毛建草,   愈伤组织诱导,   分化,   增殖,   生根


Abstract: Dracocephalum rupestre field leaves and tissue-cultured seedling leaves were used as explants to investigate the impact of hormones on callus induction, differentiation, shoot proliferation, and rooting. The aim was to establish a leaf explant regeneration system. Results indicated that the optimal medium for inducing callus from field leaves was MS+1 mg·L−1 6-BA+1 mg·L−1 2,4-D+0.1 mg·L−1 IAA, achieving an induction rate of 84.51%. For adventitious bud differentiation, the preferred medium comprised MS+3 mg·L−1 6-BA+0.5 mg·L−1 TDZ+0.5 mg·L−1 IAA, resulting in a differentiation rate of 66.37%. Similarly, for inducing callus from tissue-cultured seedling leaves, the optimal medium included MS+2 mg·L−1 6-BA+0.1 mg·L−1 2,4-D+0.5 mg·L−1 IAA, with an induction rate of 86.73%. The medium conducive to adventitious bud differentiation consisted of MS+2 mg·L−1 6-BA+2 mg·L-1 TDZ+0.05 mg·L−1 IAA, yielding a differentiation rate of 53.48%. Furthermore, the appropriate medium for shoot proliferation was MS+2 mg·L−1 6-BA+0.05 mg·L−1 NAA, achieving a proliferation rate of 83.57%. The rooting medium was 1/2MS +0.1 mg·L−1 NAA+0.1 mg·L−1 IBA, resulting in a rooting rate of 86.97%. Tissue-cultured seedlings exhibited optimal growth in a mixed substrate of peat and vermiculite at a ratio of 1:1 (v/v). This study successfully established a leaf explant regeneration system for D. rupestre, providing valuable technical support for the conservation and rapid propagation of its germplasm resources.

Key words:

Dracocephalum rupestre,   callus induction,  differentiation,   proliferation,   rooting