大花三色堇再生体系建立

doi:10.11983/CBB24164

植物学报

大花三色堇再生体系建立

张汝鑫1, 李晨荣1, 王童欣1, 黎洁1, 李霆格1, 许慧娴1, 李梅儿1, 赵莹1, 彭婷2, 3*, 王健1*

¹热带特色林木花卉遗传与种质创新教育部重点实验室/海南省热带特色花木资源生物学重点实验室/海南大学热带农林学院, 海口 570228; ²贵州大学农学院, 贵阳550025; 3华中农业大学生命科学技术学院, 武汉 430070

收稿日期:2024-10-28 修回日期:2025-02-10 出版日期:2025-02-26 发布日期:2025-02-26
通讯作者: 彭婷，王健
基金资助:
贵州省自然科学基金(No. ZK [2022] 095)、国家自然科学基金(No.32160719, No.32060365)、海口市重点科技创新项目(No.2022-013)和海南省研究生创新科研课题(No.Qhys20230-215)

Establishment of a Regeneration System for Viola × wittrochiana

Ruxin Zhang1, Chenrong Li1, Tongxin Wang1, Jie Li1, Tingge Li1, Huixian Xu1, Meier Li1, Ying Zhao1, Ting Peng2, 3*, Jian Wang1*

¹Key Laboratory of Genetics and Germplasm Innovation of Tropical Forest Trees and Ornamental Plants, Ministry of Education/Key Laboratory of Germplasm Resources Biology of Tropical Special Ornamental Plants of Hainan/School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China; ²College of Agriculture, Guizhou University, Guiyang 550025, China; 3College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

Received:2024-10-28 Revised:2025-02-10 Online:2025-02-26 Published:2025-02-26
Contact: Ting Peng, Jian Wang

摘要/Abstract

摘要： 为构建大花三色堇(Viola × wittrochiana)植株再生体系, 以大花三色堇(Viola × wittrochiana) 8个品种的叶片和叶柄为实验材料, 筛选并确定了再生率高的品种和部位, 并以PXP的叶柄为外植体, 进行再生体系的优化。结果表明, PXP是用于再生体系建立的最佳品种, 其叶柄是最佳外植体, 诱导愈伤组织的最佳培养基为1/2MS (不含糖)+30 g∙L–1 蔗糖+1.5 mg∙L–1 2,4-D+1.5 mg∙L–1 KT, 不定芽诱导的最优培养基为1/2MS (不含糖)+30 g∙L–1 海藻糖+0.05 mg∙L–1 2,4-D+3 mg∙L–1 6-BA, 再生苗经多次诱导分化, 再生率可达67.33%±3.06%。不定芽增殖的最优培养基为MS (不含糖)+30 g∙L–1 海藻糖+0.5 mg∙L–1 2,4-D+1 mg∙L–1 6-BA, 增殖系数为3.29±0.22。再生苗在生根培养基1/2MS (不含糖)+30 g∙L–1 海藻糖+0.1 mg∙L–1 NAA上培养2周后开始生根, 生根率为84.44%±6.93%。该研究建立了分化率较高的大花三色堇再生体系, 解决了大花三色堇不定芽分化困难的难题, 为其育种改良和优良品种快繁提供了技术支持。

关键词: 大花三色堇, 叶柄, 再生体系, 组织培养, 多次诱导

Abstract: In this study, the leaves and petioles of eight varieties of Viola × wittrochiana were used as explants to screen and identify the varieties and tissues of the highest regeneration rates. Subsequently, the petioles of the PXP were chosen as explants to optimize the regeneration system, thereby establishing a complete plant regeneration protocol for V. × wittrochiana. This protocol aimed to provide a technical system for rapid propagation and genetic improvement of V. × wittrochiana. The results indicated that PXP was the optimal variety and petioles were the optimal explants for establishing the regeneration system. The optimal medium for callus induction was 1/2MS (sugar-free)+30 g∙L–1 sucrose+1.5 mg∙L–1 2,4-D+1.5 mg∙L–1 KT. The optimal medium for adventitious shoot induction was 1/2MS (sugar-free)+30 g∙L–1 trehalose+0.05 mg∙L–1 2,4-D+3 mg∙L–1 6-BA. Repeate induction could promote the regeneration rate of shoot differentiation to 67.33%±3.06%. The optimal medium for adventitious shoot proliferation was MS (sugar-free)+30 g∙L–1 trehalose+0.5 mg∙L–1 2,4-D+1 mg∙L–1 6-BA, with a proliferation coefficient of 3.29±0.22. Regenerated shoots began rooting after two weeks on rooting medium consisting of 1/2MS (sugar-free)+30 g∙L–1 trehalose+0.1 mg∙L–1 NAA, with a rooting rate of 84.44%±6.93%. This study addressed the challenges of adventitious shoot differentiation in V. × wittrochiana and established a regeneration system with high shoot differentiation rate, providing technical support for breeding and genetic improvement of the species.

Key words: Viola×wittrochiana, petiole, regeneration system, tissue culture, repeate induction

张汝鑫, 李晨荣, 王童欣, 黎洁, 李霆格, 许慧娴, 李梅儿, 赵莹1, 彭婷, 王健. 大花三色堇再生体系建立. 植物学报, DOI: 10.11983/CBB24164.

Ruxin Zhang, Chenrong Li, Tongxin Wang, Jie Li, Tingge Li, Huixian Xu, Meier Li, Ying Zhao, Ting Peng, Jian Wang. Establishment of a Regeneration System for Viola × wittrochiana. Chinese Bulletin of Botany, DOI: 10.11983/CBB24164.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB24164

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大花三色堇再生体系建立

Establishment of a Regeneration System for Viola × wittrochiana

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