植物学报 ›› 2016, Vol. 51 ›› Issue (2): 251-256.DOI: 10.11983/CBB15036

• 技术方法 • 上一篇    下一篇

多肉植物劳尔的组织培养

刘芳1†, 唐映红1, 2†, 袁有美2, 郭清泉1, 沈帆1, 陈建荣1*   

  1. 1长沙学院生物与环境工程系, 长沙 410022;
    2湖南农业大学农学院, 长沙 410128
  • 收稿日期:2015-02-16 修回日期:2015-05-06 出版日期:2016-03-01 发布日期:2016-03-31
  • 通讯作者: 刘芳
  • 基金资助:

    试管合植小植物景观及商品化

Tissue Culture of the Succulent Plant Sedum clavatum

Fang Liu1†, Yinghong Tang1, 2†, Youmei Yuan2, Qingquan Guo1, Fan Shen1, Jianrong Chen1*   

  1. 1Department of Biological and Environmental Engineering of Changsha University, Changsha 410022, China

    2Agriculture College of Hunan Agriculture University, Changsha 410128, China
  • Received:2015-02-16 Revised:2015-05-06 Online:2016-03-01 Published:2016-03-31
  • Contact: Fang Liu
  • Supported by:

    湖南省科技计划(No.2014CK4018)

摘要: 以多肉植物劳尔(Sedum clavatum)叶片为外植体, 通过诱导愈伤组织、愈伤组织分化新芽、芽生根和无菌苗移栽等步骤建立劳尔无菌苗快速繁殖体系。研究结果表明, 诱导劳尔叶片愈伤组织的最佳培养基为MS+3.0 mg·L–1 6-BA+0.1mg·L–1 NAA+1 mg·L–1 KT, 诱导率达95.7%; 愈伤组织诱导分化新芽的最佳培养基为3/4MS+3.0 mg·L–1 6-BA+0.3 mg·L–1NAA, 分化率达80%; 芽生根的最佳培养基为1/2MS+0.03 mg·L–1 NAA, 生根率可达94.89%; 采用珍珠岩:蛭石(1:2)作为基质移栽培养生根苗, 成活率达90%以上。实验成功建立了劳尔快速繁殖体系。

关键词: 劳尔, 愈伤组织诱导, 植株再生

Abstract: The leaf of Sedum clavatum was used to induce callus; through callus induction, bud induction, rooting and transplatation, aseptic-frequency regeneration systems were established. The optimal medium for induction was MS+3.0 mg·L–1 6-BA+0.1 mg·L–1 NAA+1.0 mg·L–1 KT; for redifferentiation 3/4MS+3.0 mg·L–1 6-BA+0.3mg·L–1 NAA; and for rooting 1/2MS+0.03 mg·L–1 NAA. The callus induction, redifferentiation and rooting rate was 95.7%, 80% and 94.89%, respectively. Using perlite:vermiculite (1:2) as a matrix, the transplant survival rate was >90%. We have established a high-frequency regeneration system for S. clavatum.

Key words: Sedum clavatum, callus induction, plant regeneration

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