植物学报 ›› 2019, Vol. 54 ›› Issue (4): 539-546.DOI: 10.11983/CBB18161

• 技术方法 • 上一篇    

南瓜高效再生体系的建立

郭佳,李衍素,贺超兴,闫妍,于贤昌()   

  1. 中国农业科学院蔬菜花卉研究所, 北京 100081
  • 收稿日期:2018-07-18 接受日期:2018-12-10 出版日期:2019-07-10 发布日期:2020-01-08
  • 通讯作者: 于贤昌
  • 基金资助:
    国家自然科学基金(31772363);国家现代农业产业技术体系建设专项(CARS-23-B08);中国农业科学院科技创新工程(CAAS-ASTIP-IVFCAAS);农业部园艺作物生物学与种质创制重点实验室

Establishing a High-efficiency Regeneration System in Pumpkin (Cucurbita moschata)

Jia Guo,Yansu Li,Chaoxing He,Yan Yan,Xianchang Yu()   

  1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2018-07-18 Accepted:2018-12-10 Online:2019-07-10 Published:2020-01-08
  • Contact: Xianchang Yu

摘要: 南瓜(Cucurbita moschata)再生率较低, 为建立高效的南瓜再生体系, 以南瓜子叶为外植体, 进行35组不同激素浓度的不定芽诱导研究。结果表明, 南瓜再生受培养基中激素浓度和配比的影响, 适宜浓度6-苄氨基腺嘌呤(6-BA)能有效促进不定芽形成; 单独使用脱落酸(ABA)诱导使南瓜子叶发黄, 但与6-BA组合使用可显著提高外植体的再生能力, 1.0 mg∙L -16-BA与0.5 mg∙L -1ABA组合南瓜芽再生率高达90.26%。将不定芽置于MS培养基中进行生根培养, 再生苗移栽易成活。从子叶接种到苗再生约需70天。

关键词: 子叶, 植物激素, 植株再生, 南瓜

Abstract: To improve regeneration efficiency of pumpkin (Cucurbita moschata), a high-efficiency regeneration system of plantlets was established. Cotyledons were used as explants and cultured on MS supplemented with 35 concentrations of phytohormone to induce shoots. The frequency of regeneration plantlets was affected by the concentration and proportion of phytohormone in medium. Within a certain concentration, 6-BA appeared to promote the efficiency of shoot regeneration. The addition of ABA in MS caused chlorosis of cotyledons; however, 6-BA and ABA combined could effectively promote the regeneration frequency of explants. Explants cultured on MS containing 1.0 mg∙L -1 6-BA and 0.5 mg∙L -1 ABA showed the highest frequency of shoot regeneration (90.26%). The elongated shoots were cultured on MS for rooting; plantlets appeared healthy after transplanting and nearly 70 days was required for explants to become a completely regenerated plant with the high-efficiency regeneration system for pumpkin.

Key words: cotyledons, phytohormone, plant regeneration, pumpkin