南瓜高效再生体系的建立

doi:10.11983/CBB18161

摘要/Abstract

摘要： 南瓜(Cucurbita moschata)再生率较低, 为建立高效的南瓜再生体系, 以南瓜子叶为外植体, 进行35组不同激素浓度的不定芽诱导研究。结果表明, 南瓜再生受培养基中激素浓度和配比的影响, 适宜浓度6-苄氨基腺嘌呤(6-BA)能有效促进不定芽形成; 单独使用脱落酸(ABA)诱导使南瓜子叶发黄, 但与6-BA组合使用可显著提高外植体的再生能力, 1.0 mg∙L ^-16-BA与0.5 mg∙L ^-1ABA组合南瓜芽再生率高达90.26%。将不定芽置于MS培养基中进行生根培养, 再生苗移栽易成活。从子叶接种到苗再生约需70天。

关键词: 子叶, 植物激素, 植株再生, 南瓜

Abstract: To improve regeneration efficiency of pumpkin (Cucurbita moschata), a high-efficiency regeneration system of plantlets was established. Cotyledons were used as explants and cultured on MS supplemented with 35 concentrations of phytohormone to induce shoots. The frequency of regeneration plantlets was affected by the concentration and proportion of phytohormone in medium. Within a certain concentration, 6-BA appeared to promote the efficiency of shoot regeneration. The addition of ABA in MS caused chlorosis of cotyledons; however, 6-BA and ABA combined could effectively promote the regeneration frequency of explants. Explants cultured on MS containing 1.0 mg∙L ^-1 6-BA and 0.5 mg∙L ^-1 ABA showed the highest frequency of shoot regeneration (90.26%). The elongated shoots were cultured on MS for rooting; plantlets appeared healthy after transplanting and nearly 70 days was required for explants to become a completely regenerated plant with the high-efficiency regeneration system for pumpkin.

Key words: cotyledons, phytohormone, plant regeneration, pumpkin

郭佳,李衍素,贺超兴,闫妍,于贤昌. 南瓜高效再生体系的建立. 植物学报, 2019, 54(4): 539-546.

Jia Guo,Yansu Li,Chaoxing He,Yan Yan,Xianchang Yu. Establishing a High-efficiency Regeneration System in Pumpkin (Cucurbita moschata). Chinese Bulletin of Botany, 2019, 54(4): 539-546.

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https://www.chinbullbotany.com/CN/Y2019/V54/I4/539

图/表 6

参考文献 37

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Source	Type III Sum of squares	df	Mean square	F	Sig.
Corrected model	1.194^a	10	0.119	19.893	0.000
Intercept	11.738	1	11.738	1955.511	0.000
6-BA concentration	0.932	5	0.186	31.040	0.000
ABA concentration	0.291	5	0.058	9.699	0.000
Error	0.144	24	0.006
Total	13.664	35
Corrected total	1.338	34

Source	Type III Sum of squares	df	Mean square	F	Sig.
Corrected model	1.194^a	10	0.119	19.893	0.000
Intercept	11.738	1	11.738	1955.511	0.000
6-BA concentration	0.932	5	0.186	31.040	0.000
ABA concentration	0.291	5	0.058	9.699	0.000
Error	0.144	24	0.006
Total	13.664	35
Corrected total	1.338	34

Source	Type III Sum of squares	df	Mean square	F	Sig.
Corrected model	20.734^a	10	2.073	6.731	0.000
Intercept	116.473	1	116.473	378.098	0.000
6-BA concentration	11.488	5	2.298	7.458	0.000
ABA concentration	10.032	5	2.006	6.513	0.001
Error	7.393	24	0.308
Total	151.118	35
Corrected total	28.127	34

Source	Type III Sum of squares	df	Mean square	F	Sig.
Corrected model	20.734^a	10	2.073	6.731	0.000
Intercept	116.473	1	116.473	378.098	0.000
6-BA concentration	11.488	5	2.298	7.458	0.000
ABA concentration	10.032	5	2.006	6.513	0.001
Error	7.393	24	0.308
Total	151.118	35
Corrected total	28.127	34

Treatments	6-BA (mg∙L^-1)	Number of explants	Number of explants with adventitious shoots	Number of shoots	Average number of induced shoots	Rate of plant regeneration (%)
M1	0.5	60	39.78	73.60	1.85	66.31
M2	1.0	60	41.51	72.22	1.74	69.18
M3	1.5	60	34.01	50.00	1.47	56.69
M4	2.0	60	29.88	33.46	1.12	49.79
M5	2.5	60	27.16	31.50	1.16	45.26