水稻茎秆脆性及叶尖枯死突变体fld1的鉴定与基因定位

doi:10.3724/SP.J.1259.2014.00663

植物学报 ›› 2014, Vol. 49 ›› Issue (6): 663-671.DOI: 10.3724/SP.J.1259.2014.00663 cstr: 32102.14.SP.J.1259.2014.00663

水稻茎秆脆性及叶尖枯死突变体fld1的鉴定与基因定位

蒋钰东, 何沛龙, 廖红香, 张孝波, 吴国超, 何光华, 林婷婷, 桑贤春^*

西南大学水稻研究所, 转基因植物与安全控制重庆市重点实验室, 重庆 400716

收稿日期:2013-08-27 修回日期:2014-03-17 出版日期:2014-11-01 发布日期:2014-11-21
通讯作者: 桑贤春
基金资助:
国家自然科学基金

Identification and Gene Mapping of a Fragile and Leaf-tip Dead Mutant fld1 in Oryza sativa

Yudong Jiang, Peilong He, Hongxiang Liao, Xiaobo Zhang, Guochao Wu, Guanghua He, Tingting Lin, Xianchun Sang^*

Rice Research Institute/Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Southwest University, Chongqing 400716, China

Received:2013-08-27 Revised:2014-03-17 Online:2014-11-01 Published:2014-11-21
Contact: Xianchun Sang
Supported by:
Natural Science Foundation of China

摘要/Abstract

摘要： 用EMS诱变籼型水稻(Oryza sativa)恢复系缙恢10号, 获得稳定遗传的脆性叶尖枯死突变体fld1, 苗期植株呈现脆性和叶尖枯死, 机械强度显著下降, 一直持续到成熟。突变体fld1茎秆中的纤维素和木质素含量仅分别为野生型的67.92%和50.16%, 差异达极显著水平。与野生型相比, fld1叶片中的光合色素含量呈现一定程度的下降。其中, 类胡萝卜素含量在衰老和正常部位均极显著降低, 净光合速率(P_n)、气孔导度(G_s)和蒸腾速率(T_r)也极显著降低, 胞间CO₂浓度(C_i)则极显著升高。遗传分析表明, fld1的脆性和叶尖枯死性状共分离, 且受1对隐性核基因调控。利用西农1A/fld1和fld1/日本晴的F₂群体, 最终将FLD1定位在第9染色体Indel标记Ind09-2与Ind09-3之间215 kb的物理距离内, 包含33个注释基因。研究结果为下一步基因的克隆和功能研究奠定了基础。

关键词: 水稻, 脆性, 叶尖枯死, 基因定位

Abstract: A fragile and leaf-tip dead mutant, fld1, was identified from the progeny of the restorer line Jinhui10 with seeds treated with ethyl methanesulfonate (EMS). The mutant showed stable inherited traits of brittleness as well as dry and withered leaf tip throughout development. Besides showing significantly decreased mechanism strength, in fld1, cellulose and lignin content in culm occupied only 67.92% and 50.16% of that in the wild type, for a significant difference. The content of photosynthetic pigment was lower in fld1 than the wild type, as was that of carotenoid. Photosynthetic parameters of photosynthetic rate (P_n), stomatal conductance (G_s) and transpiration rate (T_r) were lower and intercellular CO₂ concentration (C_i) were higher in fld1 than the wild type. The mutational traits of brittleness and dead leaf-tip were co-segregated and controlled by one recessive nuclear gene. The FLD1 gene was mapped on chromosome 9 between Indel markers Ind09-2 and Ind09-3 with 215 kb physical distance with F₂ groups of Xinong 1A/fld1 and fld1/Nipponbare. The restricted region has 33 annotated genes. These results provide a stable foundation for FLD1 gene cloning and its application in molecular breeding.

Key words: Oryza sativa, frangibility, leaf-tip dead, gene mapping

蒋钰东, 何沛龙, 廖红香, 张孝波, 吴国超, 何光华, 林婷婷, 桑贤春. 水稻茎秆脆性及叶尖枯死突变体fld1的鉴定与基因定位. 植物学报, 2014, 49(6): 663-671.

Yudong Jiang, Peilong He, Hongxiang Liao, Xiaobo Zhang, Guochao Wu, Guanghua He, Tingting Lin, Xianchun Sang. Identification and Gene Mapping of a Fragile and Leaf-tip Dead Mutant fld1 in Oryza sativa. Chinese Bulletin of Botany, 2014, 49(6): 663-671.

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https://www.chinbullbotany.com/CN/Y2014/V49/I6/663

参考文献

[1]李文丽, 吴先军(2006).一个水稻脆性突变体的遗传分析与基因定位. 核农学报, 26, 500–502
[2]吴超, 傅亚萍, 朱丽, 胡国成, 斯华敏, 孙宗修（2008）.转高赖氨酸蛋白基因脆茎水稻的收获指数及秸秆赖氨酸含量的研究.浙江农业学报, 20, 225–230
[3]全东兴, 南钟浩, 韩龙植(2004).特种稻种质资源研究进展与展望[J]. 植物遗传资源学报, 5, 227–232
[4]桑贤春, 何光华, 张毅, 杨正林, 裴炎(2003).水稻PCR扩增模板的快速制备. 遗传, 25, 705–707
[5]徐芳芳, 桑贤春, 任德勇, 唐彦强, 胡宏伟, 杨正林, 赵芳明, 何光华(2012).水稻早衰突变体esl2的遗传分析及基因定位. 作物学报, 38, 1347–135
[6]杜青, 方立魁, 桑贤春, 凌荫华, 李云峰, 杨正林, 何光华, 赵芳明(2012).水稻叶尖早衰突变体lad 的形态、生理分析与基因定位. 作物学报, 38, 168–173
[7]苗润隆, 蒋钰东, 廖红香, 徐芳芳, 何光华, 杨正林, 赵芳明, 桑贤春(2013).水稻早衰突变体esl3的遗传分析及基因定位作物学报, 39, 862–867
[8]Yoshida S (2003).Molecular regulation of leaf senescence. Plant Boil, 6, 79–84
[9]Wu Z M, Zhang X, He B, Diao L P, Sheng Sh L, Wang J L, Guo X P, Su N, Wang L F, Jiang L, Wang Ch M, Zhai H Q, Wan J M (2007).A chlorophyll-deficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis. Plant Physiology, 145, 29–40
[10]Wang J, Wu S J, Zhou L H, Xu J F, Hu J, Fang Y X, Gu M H, Liang G H (2006).Genetic analysis and molecular mapping of a presenescing leaf gene psl1 in rice (Oryza sativa L.). Chin Sci Bull, 51, 2986–2992
[11]Zhu L, Liu W Z, Wu C, Luan W J, Fu Y P, Hu G C, Si H M, Sun Z X.Identification and fine mapping of a gene related to pale green leaf phenotype near centromere region in rice (Oryza sativa L.). Rice Sci, 2007, 14, 172–180
[12]Fang L K, Li Y F, Gong X P, Sang X C, Ling Y H, Wang X W, Cong Y F, He G H (2010).Genetic analysis and gene mapping of dominant presenescing leaf gene PSL3 in rice (Oryza sativa). Chin Sci Bull, 55, 2517–2521
[13]Aihong Lin, Yiqin Wang, Jiuyou Tang, Peng Xue, Chunlai Li, Linchuan Liu, Bin Hu, Fuquan Yang, Gary J.Loake, and Chengcai Chu(2012). Nitric Oxide and Protein S-Nitrosylation Are Integral to Hydrogen Peroxide-Induced Leaf Cell Death in Rice1[W][OA]. Plant Physiology, 158, 451–464
[14]Ben Shia, Lan Nia, Aying Zhang, Jianmei Cao, Hong Zhang, Tingting Qin, Mingpu Tan, Jianhua Zhang, and Mingyi Jiang(2012).OsDMI3 Is a Novel Component of Abscisic Acid Signaling in the Induction of Antioxidant Defense in Leaves of Rice. Molecular Plant, 5, 1359–1374
[15]Wellburn A R (1994).The Spectral Determination of Chlorophyll a and b, as well as Total Carotenoids, Using Various Solvents with Spectrophotometers of Different Resolution. Plant Physiol, 144, 307–313
[16]Li Y, Qian Q, Zhou Y, Yan M, Sun L, Zhang M, Fu Z, Wang Y, Han B, Pang X, Chen M (2003).BRITTLE CULM 1, which encodes a COBRA-like protein, affects the mechanical properties of rice plants. Plant Cell, 15, 2020–2031
[17]David M.Updegraff(1969). Semimicro determination of cellulose in biological materials. Anal Biochem, 32, 420?424
[18]Musel G, Schindler T, Bergfeld R, Ruel K, Jacquet G, Lapierre C, Speth V, Schopfer P (1997).Structure and distribution of lignin in primary and secondary cell walls of maize coleoptiles analyzed by chemical and immunological probes. Planta, 201, 146?159
[19]Michelmore R W，Paran I，Kesseli R V (1991).Identification of markers linked to disease-resistance genes by bulked segregant analysis：a rapid method to detect markers in specific genomic regions by using segregating populations．Proc Natl Acad Sci USA, 88, 9828-9832
[20]McCouch SR, Kochert G, Yu ZH.Molecular mapping of rice chromosome (1988). Theor Appl Genet, 76, 148–159
[21]Lander E S, Green P, Abrahamson J, Barlow A, Daly M J, Lin-coin S E, Newburg L (1987).MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics, 1, 174–l81
[22]Kosambi D D (1944).The estimation of map distances from recombination values. Ann Human Genet, 12, 172–175
[23]Crowell E F, Gonneau M, Stierhof Y D, H?fte H, Vernhettes S (2010).Regulated trafficking of cellulose synthases. Curr Opin Plant Biol, 13, 700–705
[24]Zhang B C, Zhou Y H (2011) .Rice brittleness mutants: A Way to open the ‘BlackBox’ of monocot cell wall biosynthesis. J Integr Plant Biol, 53(2), 136–142
[25]Wu B, Zhang B, Dai Y, Zhang L, Shang-Guan K, Peng Y, Zhou Y, Zhu Z (2012).Brittle Culm15 encodes a membrane-associated chitinase-like protein required for cellulose biosynthesis in rice. Plant Physiol, 159(4), 1440–1452
[26]Zhang B C, Liu X L, Qian Q, Liu L F, Dong G J, Xiong G Y, Zeng D L, Zhou Y H (2011).Golgi nucleotide sugar transporter modulates cell wall biosynthesis and plant growth in rice. Proc Natl Acad Sci USA, 108(12), 5110–5115
[27]Hirano K, Kotake T, Kamihara K, Tsuna K, Aohara T, Kaneko Y, Takatsuji H, Tsumuraya Y, Kawasaki S (2010).Rice BRITTLE CULM 3 (BC3) encodes a classical dynamin OsDRP2B essential for proper secondary cell wall synthesis. Planta, 232, 95–108
[28]Changjie Yan, Song Yan, Xiuhong Zeng, Zhengqiu Zhang, Minghong Gu (2007).Fine Mapping and Isolation of Bc7(t), Allelic to OsCesA4. Journal of Genetics and Genomics, 34(11), 1019–1027
[29]Yihua Zhou, Shengben Li, Qian Qian, Dali Zeng, Mu Zhang, Longbiao Guo, Xiangling Liu, Baocai Zhang, Lingwei Deng, Xinfang Liu, Guanzheng Luo, Xiujie Wang, and Jiayang Li (2009).BC10, a DUF266-containing and Golgi-located type II membrane protein, is required for cell-wall biosynthesis in rice (Oryza sativa L.). The Plant Journal, 57, 446–462
[30]Mu Zhang, BaoCai Zhang, Qian Q, Yanchun Yu, Rui Li, Junwen Zhang, Xiangling Liu, Dali Zeng, Jiayang Li, and Yihua Zhou (2010).Brittle Culm 12, a dual-targeting kinesin-4 protein, controls cell-cycle progression and wall properties in rice. Plant J, 63, 312–328
[31]Toshihisa Kotake, Tsutomu Aohara, Shinji Kawasaki, Ko Hirano, Ami Sato, Yasuko Kaneko, Yoichi Tsumuraya, Hiroshi Takatsuji and Shinji Kawasaki (2011).Rice Brittle culm 6 encodes a dominant-negative form of CesA protein that perturbs cellulose synthesis in secondary cell walls. Journal of Experimental Botany, 62( 6), 2053–2062
[32]Katsuyuki Tanaka, Kazumasa Murata, Hirohiko Hirochika (2003).Three Distinct Rice Cellulose Synthase Catalytic Subunit Genes Required for Cellulose Synthesis in the Secondary Wall. Plant Physiology, 133, 73–83
[33]Badal C Saha (2003).Hemicellulose bioconversion. J Ind Microbiol Biotechnol, 30, 279–291
[34]Fuzhen Li, Guocheng Hu, Yaping Fu, Huamin Si, Xuemei Bai, and Zongxiu Sun (2005).Genetic analysis and high-resolution mapping of a premature senescence gene Pse(t) in rice (Oryza sativa L.).Genome, 48, 738–746

水稻茎秆脆性及叶尖枯死突变体fld1的鉴定与基因定位

Identification and Gene Mapping of a Fragile and Leaf-tip Dead Mutant fld1 in Oryza sativa

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