CRISPR/Cas9技术在水稻育种中的基因编辑实验教学设计

doi:10.11983/CBB25080

摘要/Abstract

摘要： CRISPR/Cas9技术作为一种高效的基因组编辑工具，在生命科学领域的应用已经变得越来越广泛。将这一技术引入大学的实验教学中，能够帮助学生更深入地理解前沿科研进展，增强科研创新实践能力。本文设计了一个创新性教学实验，“利用CRISPR/Cas9技术研究敲除水稻SSA1(seedling stage albino1)基因对表型的影响”。该实验在帮助学生加深理解CRISPR/Cas9技术的原理和实验流程，直观认识到SSA1基因在水稻生长发育中发挥的重要作用的同时，提高学生实验操作的能力和严谨的科研思维，培养学生的探索能力和创新精神。

关键词: CRISPR/Cas9, 基因编辑, 水稻, 实验教学设计

Abstract: CRISPR/Cas9 technology, as an efficient genome editing tool, has gained increasingly widespread application in the life sciences. Incorporating this technology into university experimental teaching enables students to deepen their understanding of cutting-edge research advances while enhancing their innovative research capabilities and practical skills. In this paper, we have designed an innovative teaching experiment, “Using CRISPR/Cas9 technology to study the effect of knocking out rice SSA1(seedling stage albino1)gene on phenotype”. This experiment helps students to deepen their understanding of the principles and experimental procedures of CRISPR/Cas9 technology and to recognize the important role played by the SSA1 gene in rice growth and development. At the same time, the experiment can improve students' ability of experimental operation and rigorous scientific research thinking, and cultivate students' exploratory skills and innovative spirit.

Key words: CRISPR/Cas9,, gene editing,, rice,, experimental instructional design

吕若彤寇云霞肖雨佳艾鹏飞王雁伟. CRISPR/Cas9技术在水稻育种中的基因编辑实验教学设计. 植物学报, DOI: 10.11983/CBB25080.

Ruo-Tong LV. Experimental teaching design for gene editing in rice breeding by CRISPR/Cas9 technology. Chinese Bulletin of Botany, DOI: 10.11983/CBB25080.

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