荒漠植物红砂AP2/ERF基因家族鉴定及在盐胁迫下的表达模式

doi:10.11983/CBB25066

摘要/Abstract

摘要： 红砂(Reaumuria soongorica)是我国西北荒漠区广泛分布的耐盐旱生小灌木, 具有极强的生境适应性, 是研究植物抗逆分子机制的理想材料。本研究以红砂基因组为参考, 鉴定了其AP2/ERF基因家族成员, 并进行了系统发育、基因结构、保守基序、顺式作用元件及基因复制事件等分析, 同时对该家族成员在盐胁迫下的表达模式进行了分析。结果表明, 红砂基因组中有70个AP2/ERF基因, 系统发育分析显示它们归类为AP2、ERF、DREB和RAV亚家族; 它们的启动子区有多种光响应、胁迫响应、生长发育和激素响应等顺式作用元件。红砂AP2/ERF基因非均匀分布在其基因组的11条染色体上, 其中的63个由不同基因复制事件演化而来, 全基因组复制和分散复制是该基因家族扩张的主导因素。在盐胁迫下, 红砂AP2/ERF基因呈现不同表达模式, 6个差异表达显著, 可能在该植物响应盐胁迫过程中发挥积极作用。本研究在基因组水平鉴定和分析了红砂AP2/ERF基因家族的特征, 为进一步解析它们在该植物适应生境中的分子功能奠定了基础。

关键词: AP2/ERF基因家族, 表达分析, 全基因组鉴定, 红砂, 盐胁迫

Abstract: INTRODUCTION: Reaumuria soongorica, a perennial small shrub, is widely distributed in arid desert regions of northwestern China. It exhibits exceptional drought and salt tolerance, making it an ideal model for studying molecular mechanisms of plant stress resistance.
RATIONALE: We used the R. soongorica genome as a reference to identify members of the ‌AP2/ERF gene family‌ in this species, and ‌analyzed‌ their phylogeny, gene structure, conserved motifs, cis-acting elements, gene duplication events, ‌as well as‌ the expression patterns of these family members under salt stress.
RESULTS: ‌Seventy AP2/ERF genes‌ were identified from the R. soongorica genome. Phylogenetic analysis classified these genes into ‌four subfamilies: AP2, ERF, DREB, and RAV‌. Cis-acting element analysis revealed multiple regulatory elements associated with ‌light responsiveness, stress adaptation, growth regulation, and hormone signaling‌ in the promoter regions of R. soongorica AP2/ERF genes. These genes exhibited ‌an uneven distribution‌ across all 11 chromosomes of the R. soongorica genome, with ‌63 genes (90% of the total)‌ originating from gene duplication events. Evolutionary analysis suggested that ‌whole-genome duplication (WGD) and dispersed duplication‌ were the primary drivers of family expansion. Under salt stress, AP2/ERF genes showed ‌divergent expression patterns‌ in R. soongorica seedlings, with ‌six genes displaying significant differential expression‌ (|log2FC|≥1, P<0.05), implicating their potential roles in salt stress response.
CONCLUSION: This study identified and characterized the AP2/ERF gene family in R. soongorica at the genomic level, thereby establishing a foundation for elucidating its functional roles in this species' adaptation to arid and saline environments.

Phylogenetic tree of the AP2/ERF gene family in R. soongorica.

Key words: AP2/ERF gene family, expression analysis, genome-wide identification, Reaumuria soongarica, salt stress

朱梦璇, 刘渊, 赵颢宇, 孙振云, 巩薇, 党振华. 荒漠植物红砂AP2/ERF基因家族鉴定及在盐胁迫下的表达模式. 植物学报, DOI: 10.11983/CBB25066.

Mengxuan Zhu, Yuan Liu, Haoyu Zhao, Zhenyun Sun, Wei Gong, Zhenhua Dang. Genome-wide Identification of the AP2/ERF Gene Family and Its Expression Patterns under Salt Stress in the Desert Plant Reaumuria soongarica. Chinese Bulletin of Botany, DOI: 10.11983/CBB25066.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB25066

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