紫薇GolS家族基因理化特性与表达特征分析

doi:10.11983/CBB24118

摘要/Abstract

摘要： 植物肌醇半乳糖苷合成酶(galactinol synthase, GolS) 是棉子糖家族寡糖(raffinose family oligosaccharides, RFOs) 生物合成途径中的关键酶, 为棉子糖系列寡糖提供活化的半乳糖基, 调控植物体内棉子糖系列寡糖的生物合成与积累, 在植物对非生物胁迫的反应中发挥重要作用。然而, 关于紫薇(Lagerstroemia indica) GolS (LiGolS)基因成员的分子结构特征还未见研究报道。该研究在全基因组水平上鉴定了13个紫薇LiGolS基因成员, 并对其理化性质、染色体定位、进化关系、基因结构、保守基序以及盐胁迫下的表达量进行了分析。结果表明: 13个LiGolS基因不均匀地分布在10条染色体上, 13个LiGolS蛋白的等电点为4.75−9.45, 分子量变化范围为37.69−46.12 kDa, 氨基酸数量为327−404 aa; 亚细胞定位预测结果发现6个蛋白定位在叶绿体上, 1个蛋白定位在线粒体, 5个蛋白定位在细胞质, 1个定位在液泡。13个基因成员含有的外显子数目为0−4。基于盐胁迫LiGolS的表达分析表明, 盐处理后所有LiGolS基因成员表现出不同程度的上调表达, 表明这些基因可能参与了紫薇的盐胁迫响应。本研究结果为后续开展紫薇GolS基因的功能解析奠定了基础。

关键词: 紫薇, GolS基因家族, 盐胁迫

Abstract: INTRODUCTION: Galactinol synthase (GolS) is a key enzyme in the biosynthetic pathway of raffinose family oligosaccharides (RFOs), providing the activated galactosyl group for the biosynthesis and accumulation of RFOs in plants. It plays an important role in plant responses to abiotic stresses. RATIONALE: Although the role of GolS in plant stress responses has been extensively studied, little is known about the molecular characteristics of the GolS gene family (LiGolS) in Lagerstroemia indica. This study aims to identify the members of the LiGolS gene family, analyze their physicochemical properties, gene structure, and expression patterns, and explore their potential functions in salt stress response. RESULTS: A total of 13 LiGolS gene family members were identified at the whole-genome level and were classified into three subfamilies based on phylogenetic relationships. These genes were unevenly distributed across 10 chromosomes. The isoelectric points of the 13 LiGolS proteins ranged from 4.75 to 9.45, with molecular weights varying from 37.69 to 46.12 kDa and amino acid counts ranging from 327 to 404. Subcellular localization prediction revealed that six proteins were localized to chloroplasts, one to mitochondria, five to the cytoplasm, and one to vacuoles. Additionally, the number of exons in the 13 gene members ranged from 0 to 4. Expression analysis under salt stress showed that all LiGolS genes were upregulated to varying degrees after salt treatment, suggesting their potential involvement in salt stress response in Lagerstroemia indica. CONCLUSION: This study systematically identified and characterized the LiGolS gene family members in Lagerstroemia indica for the first time, including their physicochemical properties, gene structure, and expression patterns. These results lay the foundation for further functional analysis of LiGolS genes and provide theoretical insights into their roles in stress responses.

Key words: Crape Myrtle, GolS gene family, Salt stress

徐田甜, 杨培建, 周晓茜, 曹怡, 陈艳红, 刘国元, 张健, 魏辉. 紫薇GolS家族基因理化特性与表达特征分析. 植物学报, 2025, 60(3): 1-0.

Tiantian Xu, Yi Cao, Peijian Yang, Fan Yang, Xiaoxi Zhou, Hui Wei, Yanhong Chen. Analysis of physicochemical characteristics and expression characteristics of lagerstroemia GolS family genes. Chinese Bulletin of Botany, 2025, 60(3): 1-0.

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https://www.chinbullbotany.com/CN/Y2025/V60/I3/1

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