植物学报 ›› 2025, Vol. 60 ›› Issue (3): 393-406.DOI: 10.11983/CBB24118  cstr: 32102.14.CBB24118

• 研究论文 • 上一篇    下一篇

紫薇GolS家族基因的理化特性与表达特征

徐田甜1,2, 杨培建1,2, 周晓茜1,2, 曹怡1,2, 陈艳红1,2, 刘国元1,2, 张健1,2, 魏辉1,2,*()   

  1. 1南通大学生命科学学院, 南通 226019
    2南通市观赏植物遗传育种重点实验室, 南通 226019
  • 收稿日期:2024-08-04 接受日期:2024-11-12 出版日期:2025-05-10 发布日期:2024-11-26
  • 通讯作者: *E-mail: 15850682752@163.com
  • 基金资助:
    江苏省林业科技创新与推广项目(LYKJ[2023]02);江苏省现代农业重大关键技术攻关项目(BE2022420);南通市基础研究(JC2023104)

Analysis of Physicochemical Characteristics and Expression Characteristics of Lagerstroemia indica GolS Family Genes

Xu Tiantian1,2, Yang Peijian1,2, Zhou Xiaoxi1,2, Cao Yi1,2, Chen Yanhong1,2, Liu Guoyuan1,2, Zhang Jian1,2, Wei Hui1,2,*()   

  1. 1School of Life Sciences, Nantong University, Nantong 226019, China
    2Nantong Key Laboratory of Ornamental Plant Genetics and Breeding, Nantong 226019, China
  • Received:2024-08-04 Accepted:2024-11-12 Online:2025-05-10 Published:2024-11-26
  • Contact: *E-mail: 15850682752@163.com

摘要: 植物肌醇半乳糖苷合成酶(GolS)是棉子糖家族寡糖(RFOs)生物合成途径中的关键酶, 为棉子糖系列寡糖提供活化的半乳糖基, 调控植物体内棉子糖系列寡糖的生物合成与积累, 在植物响应非生物胁迫中发挥重要作用。然而, 关于紫薇(Lagerstroemia indica) GolS (LiGolS)家族基因的分子结构特征未见报道。该研究在紫薇全基因组水平鉴定了13个LiGolS基因, 并对其理化性质、染色体定位、进化关系、基因结构、保守基序以及盐胁迫下的表达进行分析。结果表明, 13个LiGolS基因不均匀地分布在10条染色体上, 13个LiGolS蛋白的等电点为4.75-9.45, 分子量为37.69-46.12 kDa, 氨基酸数量为327-404 aa。亚细胞定位预测结果表明, 6个蛋白定位在叶绿体, 1个蛋白定位在线粒体, 5个蛋白定位在细胞质, 1个蛋白定位在液泡。13个基因含有的外显子数目为0-4。盐胁迫下LiGolS的表达分析表明, LiGolS家族基因均表现出不同程度的表达上调, 表明这些基因可能参与紫薇的盐胁迫响应。研究结果为解析紫薇GolS基因功能奠定了基础。

关键词: 紫薇, LiGolS, 盐胁迫

Abstract: INTRODUCTION: Galactinol synthase (GolS) is a key enzyme in the biosynthetic pathway of raffinose family oligosaccharides (RFOs), providing the activated galactosyl group for the biosynthesis and accumulation of RFOs in plants. It plays an important role in plant responses to abiotic stresses. RATIONALE: Although the role of GolS in plant stress responses has been extensively studied, little is known about the molecular characteristics of the GolSgene family in Lagerstroemia indica. This study aims to identify the members of the LiGolS gene family, analyze their physicochemical properties, gene structure, and expression patterns, and explore their potential functions in salt stress response.RESULTS: A total of 13 LiGolS gene family members were identified at the whole-genome level. These genes were unevenly distributed across 10 chromosomes. The isoelectric points of the 13 LiGolS proteins ranged from 4.75 to 9.45, with molecular weights varying from 37.69 to 46.12 kDa, and amino acid counts ranging from 327 to 404. Subcellular localization prediction revealed that six proteins were localized to chloroplasts, one to mitochondria, five to the cytoplasm, and one to vacuoles. Additionally, the number of exons in the 13 gene members ranged from 0 to 4. Expression analysis under salt stress showed that all LiGolS genes were upregulated to various degrees after salt treatment, suggesting their potential involvement in salt stress response in L. indica.CONCLUSION: This study systematically identified and characterized the LiGolS gene family members in L. indica, including their physicochemical properties, gene structure, and expression patterns. These results lay the foundation for further functional analysis of LiGolS genes and provide theoretical insights into their roles in stress responses.

Expression patterns of LiGolS genes in Lagerstroemia indica under salt treatment
(A) Cloud and rain map of LiGolS gene expression in salt-sensitive L. indica cultivars under normal and salt treatment conditions; (B) Cloud and rain map of LiGolS gene expression in salt-tolerant L. indica cultivars under normal and salt treatment conditions; (C) LiGolS gene expression in different varieties of L. indica under normal and salt treatment conditions; (D) Heatmap of LiGolS gene expression patterns before and under salt treatment, this heatmap illustrated the log2-transformed expression levels of LiGolS genes before and after salt treatment (yellow modules indicate higher expression levels, while green modules indicate lower expression levels). M-CK and N-CK indicate salt-sensitive and salt-tolerant cultivars under normal conditions, respectively; M-T and N-T indicate salt-sensitive and salt-tolerant groups under salt treatment conditions, respectively.

Key words: Lagerstroemia indica, LiGolS, salt stress