植物学报 ›› 2024, Vol. 59 ›› Issue (2): 231-244.DOI: 10.11983/CBB23065  cstr: 32102.14.CBB23065

• 研究报告 • 上一篇    下一篇

糜子SBP基因家族全基因组鉴定及表达分析

段政勇1, 丁敏1, 王宇卓1, 丁艺冰2, 陈凌3, 王瑞云1,3,*(), 乔治军3,*()   

  1. 1山西农业大学农学院, 太谷 030801
    2山西农业大学玉米研究所, 忻州 034000
    3山西农业大学农业基因资源研究中心/农业农村部黄土高原作物基因资源与种质创制重点实验室/杂粮种质资源发掘与遗传改良山西省重点实验室, 太原 030031
  • 收稿日期:2023-05-21 接受日期:2023-12-25 出版日期:2024-03-10 发布日期:2024-03-10
  • 通讯作者: * 王瑞云, 山西农业大学农学院教授, 博士生导师, 山西省杂粮产业技术体系岗位专家, 中国作物学会粟类作物专业委员会委员。长期从事糜子种质资源遗传多样性研究。以通讯作者和第一作者身份发表学术论文80篇(其中SCI收录期刊8篇, 国家一级学术期刊11篇); 独著1部, 参编合著论著2部。E-mail: wry925@126.com;乔治军, 山西省农业科学院(山西农业大学)研究员, 博士生导师, “十三五”“十四五”国家谷子高粱现代农业产业技术体系谷子糜子生理岗位科学家。长期从事农作物种质资源的搜集、保存、鉴定、评价和利用以及旱作栽培生理研究。以通讯作者和第一作者身份发表论文70余篇; 其中SCI收录期刊6篇, 国家一级学术期刊10余篇, 核心期刊30余篇。目前研究团队以糜子为模式, 利用遗传学、基因组学、统计学、进化生物学和计算机科学等跨学科手段, 通过研究方法的不断创新, 旨在阐明作物品种优化的遗传机制; 通过整合生物信息学和系统生物学方法揭示作物在不同环境下的适应性和产量形成的遗传基础。E-mail: nkypzs@126.com
  • 基金资助:
    国家现代农业产业技术体系建设专项(CARS-06-14.5-A16);山西省现代农业产业技术体系建设(杂粮)项目(2023CYJSTX03- 12);山西省重点研发项目(2022ZDYF110);国家自然科学基金(31271791)

Genome-wide Identification and Expression Analysis of SBP Genes in Panicum miliaceum

Zhengyong Duan1, Min Ding1, Yuzhuo Wang1, Yibing Ding2, Ling Chen3, Ruiyun Wang1,3,*(), Zhijun Qiao3,*()   

  1. 1College of Agronomy, Shanxi Agricultural University, Taigu 030801, China
    2Corn Research Institute, Shanxi Agricultural University, Xinzhou 034000, China
    3Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops/ Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture and Rural Affairs/Center for Agricultural Genetic Resources Research, Shanxi Agricultural University, Taiyuan 030031, China
  • Received:2023-05-21 Accepted:2023-12-25 Online:2024-03-10 Published:2024-03-10
  • Contact: * E-mail: wry925@126.com;nkypzs@126.com

摘要: SBP (squamosa promoter binding protein)家族广泛参与植物生长发育、信号转导及多种生理生化过程。从糜子(Panicum miliaceum)全基因组中筛选并鉴定到25个SBP基因。系统发育分析表明, PmSBP家族成员分为6个亚家族。同一亚家族成员具有相似的基因结构和保守基序。共线性分析表明, PmSBP与拟南芥(Arabidopsis thaliana) AtSBP和水稻(Oryza sativa) OsSBP分别形成7对和31对直系同源基因。顺式作用元件分析表明, PmSBP启动子区富含逆境胁迫、光反应及激素信号响应元件。基因表达模式分析表明, PmSBP基因表达具有明显的组织特异性、品种特异性及发育阶段特异性, 表明SBP基因在糜子生长发育中发挥重要作用。研究结果为揭示SBP基因在糜子生长发育中的生物学功能奠定了基础, 也为其它作物SBP基因研究提供了借鉴参考。

关键词: 糜子, SBP基因, 全基因组鉴定, 基因表达分析

Abstract: Squamosa promoter binding protein (SBP) gene family is widely involved in plant growth and development, signal transduction, and many physiological and biochemical processes. Here, we identified a total of 25 SBP genes in the proso millet (Panicum miliaceum) genome, which were divided into 6 subfamilies through phylogenetic analysis. The members of the same subfamily had similar gene structure and conserved motifs. The collinearity analysis revealed 7 pairs of orthologous genes with Arabidopsis thaliana AtSBP and 31 pairs with Oryza sativa OsSBP. The analysis of cis-acting elements showed that the promoter region of SBP genes in P. miliaceum was rich in elements related to stress, plant light response and plant hormone signal response. The analysis of gene expression patterns showed that the SBP genes in P. miliaceum had obvious tissue specificity, variety specificity, and developmental stage specificity, suggesting important roles of SBP genes in the growth and development of P. miliaceum. The results provide a basis for further studying the biological function of SBP gene family in the growth and development of P. miliaceum and a reference for studying the SBP genes in other crops.

Key words: Panicum miliaceum, SBP gene, whole genome identification, analysis of gene expression