植物学报 ›› 2023, Vol. 58 ›› Issue (1): 159-168.DOI: 10.11983/CBB22188

所属专题: 杂粮生物学专辑 (2023年58卷1期)

• 研究报告 • 上一篇    

山西糜子核心种质分子身份证构建

王宇卓1, 林元香1, 薛亚鹏1, 段政勇1, 王晓丹1, 陈凌2, 曹晓宁2, 王瑞云1,2,*(), 乔治军2,*()   

  1. 1山西农业大学农学院, 太谷 030801
    2山西农业大学农业基因资源研究中心, 农业农村部黄土高原作物基因资源与种质创制重点实验室, 杂粮种质资源发掘与遗传改良山西省重点实验室, 太原 030031
  • 收稿日期:2022-08-06 接受日期:2023-01-10 出版日期:2023-01-01 发布日期:2023-01-10
  • 通讯作者: *E-mail: wry925@126.com;nkypzs@126.com
  • 基金资助:
    国家自然科学基金(31271791);现代农业产业技术体系建设专项资金(CARS-06-14.5-A16);山西省研究生创新项目(2022Y331);山西省重点研发项目(2022ZDYF110)

Construction of Molecular ID Card of Core Germplasm of Hog Millet (Panicum miliaceum) in Shanxi

Yuzhuo Wang1, Yuanxiang Lin1, Yapeng Xue1, Zhengyong Duan1, Xiaodan Wang1, Ling Chen2, Xiaoning Cao2, Ruiyun Wang1,2,*(), Zhijun Qiao2,*()   

  1. 1College of Agronomy, Shanxi Agricultural University, Taigu 030801, China
    2Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops, Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture and Rural Affairs, Center for Agricultural Genetic Resources Research, Shanxi Agricultural University, Taiyuan 030031, China

摘要: 为快速鉴定糜子(Panicum miliaceum)资源, 建立分子标记检测平台, 以272份山西糜子核心种质为研究材料, 利用85对简单重复序列(SSR)引物, 应用ID Analysis 4.0软件, 构建DNA分子身份证。结果表明, 对85对SSR引物进行筛选, 发现20对引物组合(RYW67、RYW53、RYW37、RYW65、RYW62、RYW77、RYW5、RYW49、RYW84、RYW19、RYW11、RYW40、RYW54、RYW28、RYW31、RYW7、RYW16、RYW8、RYW9和RYW18)可区分272份材料。共检测到等位变异(Na) 60个, 平均每个位点检出3个; Shannon多样性指数(I)为0.957 8 (RYW16)-1.096 7 (RYW5), 平均值为1.055 2; 多态性信息含量(PIC)为0.604 4 (RYW77)-0.753 0 (RYW37), 平均值为0.692 1。利用20对引物构建山西糜子核心种质的字符串、条形码和二维码DNA分子身份证, 可为种质身份标识和溯源提供实践路径。

关键词: 糜子, 核心种质, 分子身份证, 山西, 简单重复序列

Abstract: In order to quickly identify Panicum miliaceum resources, a molecular label/ID detection platform was established. In this experiment, 272 core germplasms of hog millet in Shanxi province were used as research materials, and 85 pairs of SSR primers were used as detection tools. ID Analysis 4.0 software was used to construct the DNA molecular ID of the materials. The main results were as follows: out of the 85 pairs of SSR primers that were screened, and 20 pairs of primer combinations (RYW67, RYW53, RYW37, RYW65, RYW62, RYW77, RYW5, RYW49, RYW84, RYW19, RYW11, RYW40, RYW54, RYW28, RYW31, RYW7, RYW16, RYW8, RYW9, and RYW18) could distinguish 272 materials. A total of 60 alleles (Na) were detected, with an average of 3 distinct alleles per locus. Shannon diversity index (I) was 0.957 8 (RYW16)-1.096 7 (RYW5), with an average of 1.055 2. The polymorphism information content (PIC) was 0.604 4 (RYW77)-0.753 0 (RYW37), with an average of 0.692 1. The 20 pairs of primers were used to construct the string, bar code and two-dimensional code DNA molecular identity card of Shanxi hog millet core germplasm, providing a practical way for germplasm identification and traceability management.

Key words: hog millet, core germplasm, molecular identity card, Shanxi, simple sequence repeat