植物学报 ›› 2020, Vol. 55 ›› Issue (1): 83-89.DOI: 10.11983/CBB19213

• 技术方法 • 上一篇    下一篇

植物蛋白SUMO化修饰检测方法

曲高平(),金京波   

  1. 中国科学院植物研究所, 植物分子生理学重点实验室, 北京 100093
  • 收稿日期:2019-10-30 接受日期:2019-12-11 出版日期:2020-01-01 发布日期:2019-12-20
  • 通讯作者: 曲高平
  • 基金资助:
    国际自然科学基金(31670186)

Detection of SUMOylation in Plants

Gaoping Qu(),Jingbo Jin   

  1. Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
  • Received:2019-10-30 Accepted:2019-12-11 Online:2020-01-01 Published:2019-12-20
  • Contact: Gaoping Qu

摘要:

SUMO化是一种重要的蛋白质翻译后修饰, 对植物正常生长发育不可或缺。到目前为止已筛选到上千个可能的SUMO底物, 但由于SUMO化修饰水平普遍很低, 其生物学功能研究相对较少。该文详细描述了检测蛋白SUMO化修饰的常用方法, 包括体外和体内SUMO化实验, 以及SUMO化修饰位点的检测方法, 旨在为深入研究植物蛋白SUMO化修饰提供技术支持。

关键词: 拟南芥, SUMO, SUMO化修饰, 检测方法

Abstract:

SUMOylation, a post-translational modification, is essential for plant normal growth and development. To date, thousands of possible SUMO substrates have been identified, but due to the relatively low SUMOylation level, biological significance of the SUMOylation remains largely unknown. Here, we summarized the SUMOylation detection methods, including in vitro and in vivo SUMOylation assays, which help to understand the functions of SUMOylation in plants.

Key words: Arabidopsis, SUMO, SUMOylation, detection methods