植物学报 ›› 2025, Vol. 60 ›› Issue (4): 551-561.DOI: 10.11983/CBB24154  cstr: 32102.14.CBB24154

• 研究论文 • 上一篇    下一篇

拟南芥AtFTCD-L参与根系响应土壤紧实度的机制

刘雨函1, 曹启江1,*(), 张诗晗1, 李益慧1, 王菁1, 谭晓萌1, 刘筱儒1, 王显玲2   

  1. 1沈阳大学生命科学与工程学院, 辽宁省城市有害生物治理与生态安全重点实验室, 沈阳 110044
    2沈阳农业大学生物科学技术学院, 沈阳 110866
  • 收稿日期:2024-10-13 接受日期:2025-01-20 出版日期:2025-07-10 发布日期:2025-01-21
  • 通讯作者: *E-mail: caojiang2010@126.com
  • 基金资助:
    2024年辽宁省教育厅基本科研项目(LJ212411035019);2024年沈阳大学本科大学生创新创业训练计划项目-国家级(202411035017)

Mechanism by which AtFTCD-L is Involved in the Root Response to Soil Compaction

Yuhan Liu1, Qijiang Cao1,*(), Shihan Zhang1, Yihui Li1, Jing Wang1, Xiaomeng Tan1, Xiaoru Liu1, Xianling Wang2   

  1. 1Key Laboratory of Urban Pest Control and Ecological Security in Liaoning, College of Life Sciences and Engineering, Shenyang University, Shenyang 110044, China
    2College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China
  • Received:2024-10-13 Accepted:2025-01-20 Online:2025-07-10 Published:2025-01-21
  • Contact: *E-mail: caojiang2010@126.com

摘要: 植物根系在生长发育过程中响应各种非生物胁迫, 包括干旱、重金属、盐、冷、热以及生理性缺素等, 其中土壤结构特别是土壤紧实度会影响根系的生长与形态, 进而影响作物产量。高尔基体通过囊泡分泌参与根系的生长以及响应非生物胁迫。然而, 高尔基体如何参与根系响应土壤紧实度的机制还不清楚。前期研究发现拟南芥(Arabidopsis thaliana) AtFTCD-L定位在高尔基体反面(trans Golgi network, TGN)上, 参与囊泡的分选和/或分泌, 调节根冠外周细胞中的黏液成分。在前期研究的基础上, 模拟土壤高紧实度生长条件, 观察稳定表达PINs-GFP的纯合体拟南芥植株表型, 通过观察生长素相关荧光信号, 发现AtFTCD-L突变体根尖以及根尖细胞在纵向上短于野生型等材料, 而在横向上宽于野生型等材料, 并且细胞形态明显异常。通过对PINs相关材料进行荧光信号收集, 发现突变体植株中PIN7低表达或不表达。综上表明, AtFTCD-L在拟南芥植株根系中通过调节PIN7的分布或表达来响应土壤紧实度。研究结果为揭示植物根系响应土壤紧实度胁迫的适应机制提供了理论指导。

关键词: AtFTCD-L, 紧实度, 根尖, 拟南芥, PIN7

Abstract: INTRODUCTION: Plant roots respond to various abiotic stresses, including drought stress, heavy metal stress, salt stress, and deficiencies in essential nutrients, during their growth and development. Among these factors, soil structure, especially soil compaction, significantly affects root growth and morphology, ultimately influencing crop yield. RATIONALE: The Golgi apparatus plays a role in root growth and responds to abiotic stress through vesicle secretion. However, the mechanisms by which the Golgi apparatus contributes to the response of the root system to soil compaction remain unclear. Previous studies have demonstrated that AtFTCD-L in Arabidopsis is located on the trans-Golgi network (TGN) opposite the Golgi apparatus, and plays a role in vesicle sorting and/or secretion regulation of mucin components in the peripheral cells of the root cap. RESULTS: Compared with those of the wild type, the root tips and root tip cells of the ftcd mutant are shorter in the longitudinal direction, but wider in the transverse direction, indicating abnormal cell morphology. Analysis of fluorescent signals from PIN-GFP plants revealed that PIN7 was either not expressed or expressed at very low levels in mutants. This study provides theoretical insights into the adaptive mechanisms of plant roots in response to abiotic stress induced by soil compaction. CONCLUSION: In summary, AtFTCD-L responds to soil compaction in the roots of Arabidopsis by regulating the distribution or expression of PIN7.
Phenotypic differences in the effects of soil compaction on AtFTCD-L (WT)-, and mutant (ftcd)-mediated PIN7 regulation of root cell growth. The growth phenotypes of the root tips of the Arabidopsis lines on the 7th day.

Key words: AtFTCD-L, compaction, root tip, Arabidopsis thaliana, PIN7