拟南芥光敏色素B氨基酸位点突变对其结构与功能的影响

doi:10.11983/CBB23074

植物学报 ›› 2024, Vol. 59 ›› Issue (3): 481-494.DOI: 10.11983/CBB23074 cstr: 32102.14.CBB23074

拟南芥光敏色素B氨基酸位点突变对其结构与功能的影响

陈艳晓¹^,², 李亚萍², 周晋军³, 解丽霞², 彭永彬², 孙伟², 和亚男², 蒋聪慧², 王增兰¹, 郑崇珂²^,^*(), 谢先芝²^,^*()

¹山东师范大学生命科学学院, 济南 250014
²山东省农业科学院湿地农业与生态研究所, 济南 250100
³山东省农业科学院农作物种质资源研究所, 济南 250100

收稿日期:2023-06-05 接受日期:2023-12-19 出版日期:2024-05-10 发布日期:2024-05-10
通讯作者: *E-mail: zhengck1983@163.com; 谢先芝, 山东省农业科学院湿地农业与生态研究所研究员, 博士生导师, 山东省自然科学杰出青年基金获得者。长期从事水稻光信号转导研究和水稻抗逆品种培育。以通讯作者和第一作者身份在Nature Communications、New Phytologist和National Science Review等国内外权威学术期刊发表研究论文90余篇。目前其研究团队利用遗传学、基因组学、发育生物学等手段在水稻光信号转导机制解析和水稻抗逆功能基因挖掘等方面取得重要成果, 完善了水稻光信号转导网络, 并为水稻新品种培育提供了基因资源。xzhxie2010@163.com
基金资助:
国家自然科学基金(32070216);山东省农业科学院农业科技创新工程(CXGC2023A15)

Effect of Amino Acid Point Mutations on the Structure and Function of Phytochrome B in Arabidopsis thaliana

Yanxiao Chen¹^,², Yaping Li², Jinjun Zhou³, Lixia Xie², Yongbin Peng², Wei Sun², Yanan He², onghui Jiang², Zenglan Wang¹, Chongke Zheng²^,^*(), Xianzhi Xie²^,^*()

¹School of Life Sciences, Shandong Normal University, Jinan 250014, China
²Institute of Wetland Agriculture and Ecology, Shandong Academy of Agricultural Sciences, Jinan 250100, China
³Institute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences, Jinan 250100, China

Received:2023-06-05 Accepted:2023-12-19 Online:2024-05-10 Published:2024-05-10
Contact: *E-mail: zhengck1983@163.com; xzhxie2010@163.com

摘要/Abstract

摘要： 生物体为适应外界不断变化的光环境, 进化出不同的光受体, 其中光敏色素是一类经典的植物感受红光和远红光的受体蛋白, 其通过暗适应的Pr状态和光激活的Pfr状态之间的光转换来检测红光和远红光。植物光敏色素具有1个保守的N端感光区域和1个C端调节区域, 其中N端部分包括NTE、PAS、GAF和PHY亚结构域, C端部分包括2个PAS结构域和1个组氨酸激酶相关结构域(HKRD)。为深入了解光敏色素的结构及功能, 已获得许多光敏色素功能缺失或氨基酸位点突变体, 并对其进行功能研究, 发现N端结构域在光敏色素的光谱特性、光信号感知和光信号转导等方面均具有重要作用; 而C端结构域是光敏色素的二聚化与核定位所必需。该文综述了拟南芥(Arabidopsis thaliana)中光敏色素B (phyB)各亚结构域中氨基酸位点突变对其功能的影响, 以期深入理解phyB的结构及功能, 为未来通过基因编辑手段进行作物农艺性状的遗传改良奠定基础。

关键词: 光敏色素B, 拟南芥, 结构域, 氨基酸位点突变, 功能

Abstract: Organisms have evolved different photoreceptors to adapt to the ever-changing conditions of the external light environment. Phytochromes (phys) are one of the classic plant photoreceptors, mainly perceiving red and far-red light. Phytochromes detect red and far-red light through the light conversion between the dark-adapted Pr state and the light-activated Pfr state. All plant phytochromes have a conserved N-terminal photoreceptor region and a C-terminal regulatory region. The N-terminal includes NTE, PAS, GAF, and PHY subdomains, while C-terminal includes two PAS domains and a histidine kinase-related domain (HKRD). In order to understand how the structure of photochromes controls its function, many function-deficient photochrome derivatives and amino acid point mutants have been obtained and studied. The N-terminal domain plays important roles in the spectral properties, light signal perception and light signal transduction of phyB. The C-terminal domain is essential for dimerization and nuclear localization of photochrome. This paper mainly reviews point mutations of amino acid in various subdomains of phyB in Arabidopsis thaliana and their effects on the function of phyB, in order to have a better understanding of the structure and functional regulation of phyB. It lays a foundation for obtaining crops with desired agronomic characteristics through gene editing.

Key words: phyB, Arabidopsis thaliana, domain, amino acid point mutation, function

陈艳晓, 李亚萍, 周晋军, 解丽霞, 彭永彬, 孙伟, 和亚男, 蒋聪慧, 王增兰, 郑崇珂, 谢先芝. 拟南芥光敏色素B氨基酸位点突变对其结构与功能的影响. 植物学报, 2024, 59(3): 481-494.

Yanxiao Chen, Yaping Li, Jinjun Zhou, Lixia Xie, Yongbin Peng, Wei Sun, Yanan He, onghui Jiang, Zenglan Wang, Chongke Zheng, Xianzhi Xie. Effect of Amino Acid Point Mutations on the Structure and Function of Phytochrome B in Arabidopsis thaliana. Chinese Bulletin of Botany, 2024, 59(3): 481-494.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB23074

https://www.chinbullbotany.com/CN/Y2024/V59/I3/481

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结构域	突变位点	光敏感性	发色团组装及结合	光敏色素稳定性	光小体形成	光敏色素核定位	光信号传递	参考文献
NTE	D64N	-						Oka et al., 2008
	S80A					-		Zhao et al., 2023
	S86D(A)	-(+)		o(o)			-	Medzihradszky et al., 2013; Viczián et al., 2020
PAS	Y104E	-					-	Nito et al., 2013
	S106A					-	-	Zhao et al., 2023
	R110Q	-					-	Oka et al., 2008; Kikis et al., 2009
	G111D	-					-	Oka et al., 2008; Kikis et al., 2009
	G112D	-					-	Oka et al., 2008
	G118R		-					Oka et al., 2008
	C119Y		-				-	Kikis et al., 2009
	S134G	-	-				-	Oka et al., 2008; Kikis et al., 2009
	I143L	+						Ruiz-Diaz et al., 2022
PAS	P149L	-						Oka et al., 2008
PAS	I208T	-	o					Oka et al., 2008
	S227A			+			-	Liu et al., 2023
GAF	L256C		-					Kikis et al., 2009
	V264E		-					Kikis et al., 2009
	V273L		-					Kikis et al., 2009
	Y276H	+					+	Su and Lagarias, 2007; Bae and Choi, 2008; Hu et al., 2009
	H283T			-				Reed et al., 1993; Oka et al., 2008
	G284E	-						Oka et al., 2008
	P304L	o		o			-	Oka et al., 2008
	I308T		o					Kikis et al., 2009
	P309L	-						Oka et al., 2008
	R313K	-	o	-				Oka et al., 2008
	R322Q	-	o	-				Oka et al., 2008
	C327Y			-				Chen et al., 2003; Oka et al., 2008
	S343Y		-					Kikis et al., 2009
	C345R		-					Kikis et al., 2009
	R352K	o		o			-	Oka et al., 2008
	C357S		-					Wagner et al., 1996
	S367P		o					Kikis et al., 2009
	S370F	-						Oka et al., 2008
	A372T	-		-				Chen et al., 2003; Oka et al., 2008
	V401I	-	o	-				Oka et al., 2008
	P411L		-					Kikis et al., 2009
	R415W		-					Kikis et al., 2009
PHY	D453R	+		+				Viczian et al., 2020
	G564E(A)	+	o	+			+(-)	Kretsch et al., 2000; Oka et al., 2008
	M579T	-						Maloof et al., 2001; Burgie et al., 2014
	R582A	+		+				Burgie et al., 2014
	S584F			-				Oka et al., 2008
	A587T			-		-		Chen et al., 2003; Oka et al., 2008
PRD	G674D	-			-			Chen et al., 2003
	A719V	-			-			Chen et al., 2003
	A750V	-					-	Wagner and Quail, 1995
	G767E(R)	-(-)					-(-)	Wagner and Quail, 1995; Matsushita et al., 2003
	E812K	-		-			-	Wagner and Quail, 1995; Elich and Chory, 1997; Chen et al., 2003
HKRD	V980I	-						Ruiz-Diaz et al., 2022
	D1040V	-			-		-	Qiu et al., 2017; Kwon et al., 2018
	L1072V	-						Ruiz-Diaz et al., 2022

结构域	突变位点	光敏感性	发色团组装及结合	光敏色素稳定性	光小体形成	光敏色素核定位	光信号传递	参考文献
NTE	D64N	-						Oka et al., 2008
	S80A					-		Zhao et al., 2023
	S86D(A)	-(+)		o(o)			-	Medzihradszky et al., 2013; Viczián et al., 2020
PAS	Y104E	-					-	Nito et al., 2013
	S106A					-	-	Zhao et al., 2023
	R110Q	-					-	Oka et al., 2008; Kikis et al., 2009
	G111D	-					-	Oka et al., 2008; Kikis et al., 2009
	G112D	-					-	Oka et al., 2008
	G118R		-					Oka et al., 2008
	C119Y		-				-	Kikis et al., 2009
	S134G	-	-				-	Oka et al., 2008; Kikis et al., 2009
	I143L	+						Ruiz-Diaz et al., 2022
PAS	P149L	-						Oka et al., 2008
PAS	I208T	-	o					Oka et al., 2008
	S227A			+			-	Liu et al., 2023
GAF	L256C		-					Kikis et al., 2009
	V264E		-					Kikis et al., 2009
	V273L		-					Kikis et al., 2009
	Y276H	+					+	Su and Lagarias, 2007; Bae and Choi, 2008; Hu et al., 2009
	H283T			-				Reed et al., 1993; Oka et al., 2008
	G284E	-						Oka et al., 2008
	P304L	o		o			-	Oka et al., 2008
	I308T		o					Kikis et al., 2009
	P309L	-						Oka et al., 2008
	R313K	-	o	-				Oka et al., 2008
	R322Q	-	o	-				Oka et al., 2008
	C327Y			-				Chen et al., 2003; Oka et al., 2008
	S343Y		-					Kikis et al., 2009
	C345R		-					Kikis et al., 2009
	R352K	o		o			-	Oka et al., 2008
	C357S		-					Wagner et al., 1996
	S367P		o					Kikis et al., 2009
	S370F	-						Oka et al., 2008
	A372T	-		-				Chen et al., 2003; Oka et al., 2008
	V401I	-	o	-				Oka et al., 2008
	P411L		-					Kikis et al., 2009
	R415W		-					Kikis et al., 2009
PHY	D453R	+		+				Viczian et al., 2020
	G564E(A)	+	o	+			+(-)	Kretsch et al., 2000; Oka et al., 2008
	M579T	-						Maloof et al., 2001; Burgie et al., 2014
	R582A	+		+				Burgie et al., 2014
	S584F			-				Oka et al., 2008
	A587T			-		-		Chen et al., 2003; Oka et al., 2008
PRD	G674D	-			-			Chen et al., 2003
	A719V	-			-			Chen et al., 2003
	A750V	-					-	Wagner and Quail, 1995
	G767E(R)	-(-)					-(-)	Wagner and Quail, 1995; Matsushita et al., 2003
	E812K	-		-			-	Wagner and Quail, 1995; Elich and Chory, 1997; Chen et al., 2003
HKRD	V980I	-						Ruiz-Diaz et al., 2022
	D1040V	-			-		-	Qiu et al., 2017; Kwon et al., 2018
	L1072V	-						Ruiz-Diaz et al., 2022

模块	亚结构域	功能	主要作用
PSM	NTE	调控光敏色素Pfr形式的稳定性, 如影响光敏色素的热逆转反应; 负责光信号传递, 如影响phyB与下游转录因子PIF结合及通过光-钙调控环促进phyB入核	光信号感知及传递
	PAS	调控发色团的组装; 负责光信号传递, 如影响phyB与下游转录因子PIF3的结合能力
	GAF	负责发色团的组装, 感应光信号; 维持phyB Pr/Pfr形式正常的可逆转换; 保持光敏色素Pfr形式的数量或整体结构, 如影响phyB的暗逆转率; 负责光信号传递, 如感光结可能直接与PIF相互作用
	PHY	调控光敏色素Pfr形式的稳定性, 如影响phyB的暗逆转率以及热逆转反应; 维持phyB Pr/Pfr形式正常的可逆转换; 负责光信号传递, 如影响phyB与下游成分的相互作用及phyB的核定位
OPM	PRD	调控光敏色素Pfr形式的稳定性, 如影响phyB的暗逆转反应; 负责光信号传递, 如影响phyB的核定位	光信号传递
OPM	HKRD	介导光敏色素的二聚化, 从而影响phyB在核内积累和光小体定位中的早期信号传递	光信号传递

模块	亚结构域	功能	主要作用
PSM	NTE	调控光敏色素Pfr形式的稳定性, 如影响光敏色素的热逆转反应; 负责光信号传递, 如影响phyB与下游转录因子PIF结合及通过光-钙调控环促进phyB入核	光信号感知及传递
	PAS	调控发色团的组装; 负责光信号传递, 如影响phyB与下游转录因子PIF3的结合能力
	GAF	负责发色团的组装, 感应光信号; 维持phyB Pr/Pfr形式正常的可逆转换; 保持光敏色素Pfr形式的数量或整体结构, 如影响phyB的暗逆转率; 负责光信号传递, 如感光结可能直接与PIF相互作用
	PHY	调控光敏色素Pfr形式的稳定性, 如影响phyB的暗逆转率以及热逆转反应; 维持phyB Pr/Pfr形式正常的可逆转换; 负责光信号传递, 如影响phyB与下游成分的相互作用及phyB的核定位
OPM	PRD	调控光敏色素Pfr形式的稳定性, 如影响phyB的暗逆转反应; 负责光信号传递, 如影响phyB的核定位	光信号传递
OPM	HKRD	介导光敏色素的二聚化, 从而影响phyB在核内积累和光小体定位中的早期信号传递	光信号传递

拟南芥光敏色素B氨基酸位点突变对其结构与功能的影响

Effect of Amino Acid Point Mutations on the Structure and Function of Phytochrome B in Arabidopsis thaliana

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