拟南芥光敏色素B氨基酸位点突变对其结构与功能的影响

doi:10.11983/CBB23074

摘要/Abstract

摘要： 生物体为适应外界不断变化的光环境条件, 进化出不同的光受体, 其中光敏色素是一类经典的植物感受红光和远红光的受体蛋白, 其通过暗适应的Pr状态和光激活的Pfr状态之间的光相互转换来检测红光和远红光。植物的光敏色素都有1个保守的N端感光区域和1个C端调节区域, 其中N端部分包括NTE、PAS、GAF和PHY四个亚结构域, C端部分包括2个PAS结构域和1个组氨酸激酶相关结构域(HKRD)。为深入了解光敏色素的结构是如何控制其功能的, 已经获得许多光敏色素功能缺失或氨基酸位点突变体, 并对其进行了功能研究, 结果表明N端结构域在光敏色素的光谱特性、光信号感知和光信号转导等方面都发挥着重要作用, 而C端结构域是光敏色素的二聚化与核定位所必需的。本文主要综述了拟南芥(Arabidopsis thaliana)中phyB各个亚结构域中氨基酸位点突变对phyB功能的影响, 以期对光敏色素B的结构及功能调控有更深入的理解, 为未来通过基因编辑手段进行农艺性状的作物遗传改良奠定基础。

关键词: 光敏色素B, 拟南芥, 结构域, 氨基酸突变位点, 功能

Abstract: Organisms have evolved different photoreceptors in order to adapt to the ever-changing conditions of the external light environment. Among them, photochrome is one of classic plant photoreceptors, mainly perceiving red and far-red light. Phytochrome detects red and far-red light through the light conversion between the dark-adapted Pr state and the light-activated Pfr state. All plant phytochromes have a conserved N-terminal photoreceptor region and a C-terminal regulatory region. The N-terminal includes NTE, PAS, GAF and PHY subdomains, while C-terminal includes two PAS domains and a histidine kinase-related domain (HKRD). In order to further understand how the structure of photochromes controls its function, many photochrome-deficient derivatives and amino acid site mutants have been obtained and fully studied. It has been found that the N-terminal domain plays important roles in the spectral properties, light signal perception and light signal transduction of phyB. The C-terminal domain is believed to be essential for dimerization and nuclear localization of photochrome. This paper mainly reviews the mutations of amino acid sites in various subdomains of phyB in Arabidopsis thaliana and their effects on the function of phyB, in order to have a deeper understanding of the structure and functional regulation of phytochrome B. It lays a foundation for future crops to obtain desired physiological characteristics through gene editing.

Key words: phyB, Arabidopsis thaliana, domain, amino acid mutation sites, function

陈艳晓, 李亚萍, 周晋军, 解丽霞, 彭永彬, 孙伟, 和亚男, 蒋聪慧, 王增兰, 郑崇珂, 谢先芝. 拟南芥光敏色素B氨基酸位点突变对其结构与功能的影响 . 植物学报, 2024, 59(3): 0-0.

Yanxiao Chen, Yaping Li, Jinjun Zhou, Lixia Xie, Yongbin Peng, Wei Sun, Yanan He, Conghui Jiang, Zenglan Wang, Zhongke Zheng, Xianzhi Xie. Effect of Amino Acid Site Mutation on the Structure and Function of Phytochrome B in Arabidopsis thaliana. Chinese Bulletin of Botany, 2024, 59(3): 0-0.

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