西藏野生拟南芥开花时间变异的遗传基础

doi:10.11983/CBB23140

摘要/Abstract

摘要： 开花时间是被子植物生活史中的关键节点。十字花科的拟南芥广布于世界各地，青藏高原海拔4000米以上也发现了该物种的自然居群。高原独特的环境塑造了其生活史的独特表型，在开花时间方面表现为中等程度的早花。本研究构建了西藏拟南芥Lhasa居群的F₂代作图群体，利用基于全基因组测序的QTL-seq定位分析，在该居群中定位到了主效基因FLC，并鉴定到了其第一个内含子中存在2307 bp的缺失，这种单倍型只存在于西藏拟南芥居群。利用CRISPR-Cas9技术构建了Lhasa背景的flc^-/-突变体，表现为开花时间显著提前。以上实验结果表明，西藏拟南芥开花时间改变的主要原因是FLC非编码区的变异，该变异并未使其丧失全部功能。这种变异可能有利于西藏拟南芥适应青藏高原的独特气候环境。

关键词: 西藏拟南芥, 开花时间, Flowering Locus C, 适应性演化

Abstract: Flowering time is a critical point in the life history of angiosperm plants. Arabidopsis thaliana of the Brassicaceae is widely distributed around the world, and natural populations of this species have been found at 4,000 m altitude in the Qinghai-Tibet Plateau. The unique plateau climate has shaped their life history phenotypes, including moderately early in flowering time. In this study, we constructed F₂ mapping population and utilized whole-genome sequencing-based QTL-seq analysis to locate the major effect gene FLC in Lhasa population of A. thaliana, and identified a deletion of 2,307 bp within the first intron of FLC, which is a haplotype unique to Tibetan A. thaliana. Lhasa population flc^-/- mutant was constructed by CRISPR-Cas9 gene editing technique. The mutant exhibited significantly earlier flowering time. The above findings suggest that the variation in the first intron of FLC in Tibetan A. thaliana could be one of the main reasons for the early flowering phenotype, although it does not lose its function completely. This variation may facilitate the adaptation of Tibetan A. thaliana to the unique climatic environment of the Qinghai-Tibet Plateau.

Key words: Tibetan Arabidopsis thaliana, flowering time, Flowering Locus C, adaptive evolution

杨继轩, 王雪霏, 顾红雅. 西藏野生拟南芥开花时间变异的遗传基础. 植物学报, 2024, 59(3): 0-0.

Jixuan Yang, Xuefei Wang, Hongya Gu. Genetic Basis of Variation of Flowering Time in Tibetan Arabidopsis thaliana. Chinese Bulletin of Botany, 2024, 59(3): 0-0.

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https://www.chinbullbotany.com/CN/Y2024/V59/I3/0

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