水稻减数分裂染色体分析方法

doi:10.11983/CBB19087

摘要/Abstract

摘要： 减数分裂粗线期染色体研究技术的发展, 很大程度上克服了水稻(Oryza sativa)细胞遗传研究中较小染色体所带来的研究困难。减数分裂染色体的制备与观察已经成为水稻细胞遗传学研究中的常规方法。该文详细描述了水稻中常用的减数分裂染色体制备、荧光原位杂交和免疫荧光染色的实验方法。

关键词: 水稻, 减数分裂, 染色体, 荧光原位杂交, 免疫荧光染色

Abstract: The development of techniques to analyze pachytene chromosomes has greatly overcome most of the difficulties in cytological studies of rice chromosomes caused by their small size. Visualization of meiotic chromosomes has now become routine in cytogenetic studies of this species. This chapter provides protocols on basic meiotic chromosome preparation, FISH analysis and immunocytology in rice.

Key words: rice, meiosis, chromosome, FISH, immunostaining

程新杰, 于恒秀, 程祝宽. 水稻减数分裂染色体分析方法. 植物学报, 2019, 54(4): 503-508.

Xinjie Cheng, Hengxiu Yu, Zhukuan Cheng. Protocols for Analyzing Rice Meiotic Chromosomes. Chinese Bulletin of Botany, 2019, 54(4): 503-508.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB19087

https://www.chinbullbotany.com/CN/Y2019/V54/I4/503

图/表 1

图1 水稻减数分裂粗线期染色体 (A) 水稻花粉母细胞减数分裂粗线期染色体的荧光原位杂交结果, 红色为端粒探针pAtT4的杂交信号, 蓝色为DAPI染色的粗线期染色体(普通荧光显微镜下观察); (B) 水稻花粉母细胞减数分裂粗线期染色体的免疫荧光染色结果, 绿色为PAIR3荧光信号, 红色为ZEP1荧光信号(超分辨荧光显微镜下观察)。Bars=5 µm

Figure 1 Characterization of rice meiotic pachytene chromosomes (A) Fluorescent in situ hybridization using rice pachytene chromosomes probed with the telomeric sequence pAtT4 (red); Chromosomes stained with DAPI (blue) (Images taken with a regular fluorescence microscope); (B) Immunodetection of PAIR3 (green) and ZEP1 (red) in rice pachytene chromosomes (Images taken with a super resolution microscope). Bars=5 µm

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