利用低温水浴鉴定水稻苗期耐寒性

doi:10.11983/CBB19101

摘要/Abstract

摘要： 高效准确鉴定苗期耐寒性是水稻(Oryza sativa)耐寒研究的前提。基于流动水浴温度均一这一特性, 建立了一种恒温水浴鉴定水稻幼苗耐寒性方法。该方法中环境温度设定为20°C, 水浴温度设定为4°C。根据对2个水稻亚种不同材料的处理结果, 总结出几种常见品种的低温处理时间与存活率参考值, 并对操作过程中的一些注意事项进行了说明。

关键词: 水稻, 苗期, 耐寒, 低温水浴

Abstract: The premise of studies on rice chilling tolerance is to find an efficient and accurate way to evaluate chilling tolerance of rice seedling. In this study, we developed an efficient technology to evaluate the cold tolerance at seedling stage by using a constant temperature water bath, based on the characteristics of excellent temperature uniformity for circulation of water. In this method, the setting temperature of environment temperature and water bath was 20°C and 4°C, respectively. From the results of two subspecies (indica/xian and japonica/geng), we summarized the reference treatment time for different survival rate of cultivars. Some attention to the cold treatment procedure was also discussed.

Key words: rice, seedling, cold tolerance, low temperature water bath

刘栋峰,唐永严,雒胜韬,罗伟,李志涛,种康,徐云远. 利用低温水浴鉴定水稻苗期耐寒性. 植物学报, 2019, 54(4): 509-514.

Dongfeng Liu,Yongyan Tang,Shengtao Luo,Wei Luo,Zhitao Li,Kang Chong,Yunyuan Xu. Identification of Chilling Tolerance of Rice Seedlings by Cold Water Bath. Chinese Bulletin of Botany, 2019, 54(4): 509-514.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB19101

https://www.chinbullbotany.com/CN/Y2019/V54/I4/509

图/表 5

图1 水稻种子萌发、播种和幼苗培养方式 (A) 水稻种子萌发状态; (B) 萌发种子在96孔板上的放置方式; (C) 三叶期幼苗(Bar=2 cm)

Figure 1 Germination and sowing of rice seeds, and the way of seedling culture (A) Germination of rice seeds; (B) Placement of germination seeds in 96-well plates without bottom; (C) Trefoil stage seedlings (Bar=2 cm)

图2 幼苗低温水浴处理示意图 (A) 水浴箱24小时内温度检测结果; (B) 低温水浴箱冷处理三叶期幼苗; (C) 幼苗在低温水浴箱中放置局部放大图; (D) 低温水浴箱中幼苗放置和固定方式

Figure 2 The diagram of low temperature water bath treatment of rice seedlings (A) The temperature of water bath within 24 h; (B) Seedlings under chilling treatment in 4°C water bath; (C) Partially enlarged detail of the seedlings in the cold water bath tank; (D) A diagram of the fixation pattern of seedlings in cold water bath

图3 冷水淹没深度对水稻低温耐受性的影响红色箭头指示茎尖生长点的位置, 该部位在处理I、II和III中分别处于水深的不同位置(虚线为水面)。图中百分数表示恢复生长7天后幼苗的存活率。以28°C水浴作为对照(左图)。Bars=6 cm

Figure 3 Effect of submerged depth of cold water on low temperature tolerance of rice Red arrow indicated the site of shoot apical meristem under different water depth in chilling treatment I, II and III. Dotted lines represent the water surface. The survival rates were indicated on the top of the seedlings recoveried for 7 days. Treatment with 28oC water was used as control (left). Bars=6 cm

图4 不同水稻材料的苗期耐寒鉴定结果 (A) Dongjin (DJ)、日本晴(NIP)和明恢86 (MH86)幼苗4°C处理96小时并恢复7天(28°C)后的表型及存活率(平均值±标准差, n≥30, 3次重复); (B) ZH10和OX8低温处理(28°C)后恢复50天28°C的表型。Bars=2 cm

Figure 4 The evaluation of cold tolerance for different rice varieties at seedling stage (A) Phenotypic response to chilling in DJ, NIP and MH86, the survival rate was determined after cold-water treatment at 4°C for 96 h and subsequent recovery at 28°C for 7 days (means±SD, n≥30, three replicates); (B) The phenotype for seedlings of ZH10 and OX8 after the chilling treatment at 4°C for 120 h, subsequently at 28°C for 50 days. Bars=2 cm

表2 籼稻和粳稻常见品种苗期不同存活率的建议处理时间

Table 2 The recommended treatment time for different survival rates of cultivars of indica/xian and japonica/geng

Subspe- cies	Cultivars	Survival rate (>50%)	Survival rate (<10%)
japonica/ geng	KY131, Kitaake	60-80 h	96-120 h
	DJ, HY	72-84 h	120-144 h
	ZH10, NIP	72-96 h	120-144 h
	YD	96-144 h	168-192 h
indica/ xian	ZF802, GLA4, IR64	12-28 h	40-60 h
indica/ xian	93-11, MH86	24-40 h	48-60 h

参考文献 6

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