植物学报 ›› 2019, Vol. 54 ›› Issue (2): 217-226.DOI: 10.11983/CBB18222

所属专题: 逆境生物学专辑 (2019年54卷2期)

• 研究报告 • 上一篇    下一篇

SmGGPPS2对丹参酮合成的影响

化文平1,2,陈尘3,4,智媛2,刘莉1,王喆之2,李翠芹2,*()   

  1. 1 陕西学前师范学院生命科学与食品工程学院, 西安 710061
    2 陕西师范大学生命科学学院, 西北濒危药材资源开发国家工程实验室, 药用资源与天然药物化学教育部重点实验室, 西安 710062
    3 陕西省西安植物园, 西安 710068
    4 陕西省植物资源保护与利用工程技术研究中心, 西安 710068
  • 收稿日期:2018-10-22 接受日期:2019-02-19 出版日期:2019-03-10 发布日期:2019-09-01
  • 通讯作者: 李翠芹
  • 基金资助:
    国家自然科学基金(31800259);国家自然科学基金(31870276);国家重点研发计划“中医药现代化研究”专项(2018YFC1706504);陕西省教育厅科学研究基金(17JK0182);陕西省教育厅科学研究基金(2015JK1177);中央高校基本科研业务费专项资金(GK201706009);陕西省自然科学基础研究计划(2018JQ3033);陕西省自然科学基础研究计划(2018JZ3001)

Effect of SmGGPPS2 Expression on Tanshinones Biosynthesis in Salvia miltiorrhiza

Wenping Hua1,2,Chen Chen3,4,Yuan Zhi2,Li Liu1,Zhezhi Wang2,Cuiqin Li2,*()   

  1. 1 Department of Life Sciences and Food Technology, Shaanxi Xueqian Normal University, Xi’an 710061, China
    2 Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi’an 710062, China
    3 Xi’an Botanical Garden, Xi’an 710068, China
    4 Shaanxi Engineering Research Centre for Conservation and Utilization of Botanical Resources, Xi’an 710068, China
  • Received:2018-10-22 Accepted:2019-02-19 Online:2019-03-10 Published:2019-09-01
  • Contact: Cuiqin Li

摘要: 香叶基香叶基焦磷酸合酶(GGPPS)是植物二萜类次生代谢物合成过程中的重要调控位点。在药用模式植物丹参(Salvia miltiorrhiza)中, GGPPS基因家族成员SmGGPPS2的生物学功能及其在丹参酮有效成分合成过程中的作用尚不明确。分别在丹参植株中过表达和RNA干涉SmGGPPS2基因, 并对转基因丹参中丹参酮含量和丹参酮合成相关基因表达量 以及转基因植物生理指标进行检测, 结果表明, 过表达SmGGPPS2株系中的丹参酮IIA和铁锈醇等脂溶性成分含量高于野生型; RNA干涉SmGGPPS2株系中的丹参酮IIA和铁锈醇等脂溶性成分含量均低于野生型。调节表达SmGGPPS2后, 丹参株系中SmHMGR1SmCPS1等多个关键酶基因的表达都呈现明显的变化。此外, 调节表达SmGGPPS2还影响丹参植株抗性。以上结果表明, SmGGPPS2在丹参酮等萜类物质的合成中起重要的调控作用。

关键词: 丹参, GGPPS, 丹参酮, 基因表达

Abstract: Geranylgerany pyrophosphate synthase (GGPPS) is an important regulator in the plant diterpenoid biosynthesis pathway. The GGPPS gene family plays a critical role in the development of the medicinal model plant Salvia miltiorrhiza. However, the biological function of SmGGPPS2, especially in the biosynthesis of tanshinone or other active ingredients, is still unclear. For functional investigation, SmGGPPS2 expression was up- or down-regulated in S. miltiorrhiza plants via overexpression or RNA interference, respectively. Then we detected the content of tanshinones, the expression of genes related to tanshinone biosynthesis, and the physiological indexes of transgenic S. miltiorrhiza plants. The content of fat-soluble components, such as tanshinone IIA and ferruginol, was increased significantly in SmGG- PPS2-overexpressed lines compared with the wild type, and the content of fat soluble-components was lower in SmGGPPS2-RNAi lines than those in the wild type lines. With the regulation of SmGGPPS2, the expression of key enzyme genes related to tanshinone biosynthesis in S. miltiorrhiza, such as SmHMGR1 and SmCPS1, was changed. In addition, the regulation of SmGGPPS2 expression also affected the resistance of S. miltiorrhiza. Our results indicate that SmGGPPS2 plays an important regulatory role in tanshinone biosynthesis.

Key words: Salvia miltiorrhiza, GGPPS, tanshinone, gene expression