植物学报 ›› 2024, Vol. 59 ›› Issue (4): 0-0.DOI: 10.11983/CBB23129
• 研究报告 • 下一篇
赵来鹏1,2, 王柏柯2, 杨涛,2 李宁2, 杨海涛2, 王娟2*, 闫会转1*
收稿日期:
2023-09-15
修回日期:
2023-12-15
出版日期:
2024-07-01
发布日期:
2024-01-12
通讯作者:
王娟, 闫会转
基金资助:
农业科技创新稳定支持项目(No.xjnkywdzc-2023001)、自治区重点研发计划项目(No.2022B02032-2)、新疆特色果蔬基因组研究与遗传改良重点实验室开放课题(2023)和新疆维吾尔自治区蔬菜产业技术体系
Laipeng Zhao1,2, Baike Wang2, Tao Yang2, Ning Li2, Haitao Yang2, Juan Wang2*, Huizhuan Yan1*
Received:
2023-09-15
Revised:
2023-12-15
Online:
2024-07-01
Published:
2024-01-12
Contact:
Juan Wang,Huizhuan Yan
摘要: 植物在生长发育过程中面临周围环境带来的各种非生物胁迫。其中干旱胁迫严重影响作物生长, 降低其产量。在植物中具有以TB2/DP1结构域为特征的HVA22蛋白参与调控植物的生长发育和非生物胁迫响应, 然而在番茄干旱胁迫响应中的功能并不清楚。因此, 在本研究中, 我们探索了番茄SlHVA22l基因的功能。研究表明, 番茄SlHVA22l与其他双子叶植物中的HVA22l同源蛋白具有较高的序列相似性, 且SlHVA22l基因表达受干旱胁迫和植物激素(ABA和MeJA)诱导。同时, 通过酵母(Saccharomyces
cerevisiae)异源表达初步探明了SlHVA22l基因的抗旱功能。此外, 通过VIGS技术沉默番茄内源SlHVA22l基因的植株对干旱敏感。沉默植株经干旱处理后表现出较高的H2O2和MDA含量, 以及较低的O2-清除率, 且沉默植株中的SOD、POD、CAT和APX活性较对照植株均显著降低。综上所述, 这些结果表明SlHVA22l基因在番茄抵御干旱胁迫过程中发挥了重要作用。
赵来鹏, 王柏柯, 杨涛, 李宁, 杨海涛, 王娟, 闫会转. SlHVA22l基因调节番茄耐旱性研究. 植物学报, 2024, 59(4): 0-0.
Laipeng Zhao, Baike Wang, Tao Yang, Ning Li, Haitao Yang, Juan Wang, Huizhuan Yan. Investigation of SlHVA22l gene regulating drought tolerance in tomato. Chinese Bulletin of Botany, 2024, 59(4): 0-0.
[1]Casaretto JA, Ho T-hD(2005).Transcriptional regulation by abscisic acid in barley (Hordeum vulgare Lseeds involves autoregulation of the transcription factor HvABI5.Plant Molecular Biology, 57:21-34. [2]Chaudhary J, Khatri P, Singla P, Kumawat S, Kumari A, Vikram A, Jindal SK, Kardile H, Kumar R, Sonah H (2019)(2019).Advances in omics approaches for abiotic stress tolerance in tomato.Biology, 8:90-109. [3]Chen C-N, Chu C-C, Zentella R, Pan S-M, David Ho T-H(2002).AtHVA22 gene family in Arabidopsis: phylogenetic relationship,ABA and stress regulation,and tissue-specific expression.Plant molecular biology, 49:631-642. [4]Choudhury FK, Rivero RM, Blumwald E, Mittler R(2017).Reactive oxygen species,abiotic stress and stress combination.The Plant Journal, 90:856-867. [5]De Antoni A, Schmitzová J, Trepte H-H, Gallwitz D, Albert St(2002).Significance of GTP hydrolysis in Ypt1p-regulated endoplasmic reticulum to Golgi transport revealed by the analysis of two novel Ypt1-GAPs.Journal of Biological Chemistry, 277:41023-41031. [6]Eggert E, Obata T, Gerstenberger A, Gier K, Brandt T, Fernie AR, Schulze W, Kühn C(2016).A sucrose transporter‐interacting protein disulphide isomerase affects redox homeostasis and links sucrose partitioning with abiotic stress tolerance.Plant, cell & environment, 39:1366-1380. [7]Ferreira MDG, Castro JA, Silva RJS, Micheli F(2019).HVA22 from citrus: A small gene family whose some members are involved in plant response to abiotic stress.Plant Physiology and Biochemistry, 142:395-404. [8]Fukuda T, Saigusa T, Furukawa K, Inoue K, Yamashita S-I, Kanki T (2023)(2023).Hva22, a REEP family protein in fission yeast, promotes reticulophagy in collaboration with a receptor protein. Autophagy, .Autophagy, 19:2657-2667. [9]Grzesiak MT, Hordyńska N, Maksymowicz A, Grzesiak S, Szechyńska-Hebda M (2019).Variation among spring wheat (triticum aestivum l.) genotypes in response to the drought stress. II—Root system structure. Plants 8, 584.., :-. [10]Guo W-J, David Ho T-H(2008).An abscisic acid-induced protein,HVA22,inhibits gibberellin-mediated programmed cell death in cereal aleurone cells.Plant physiology, 147:1710-1722. [11]Hu J, Shibata Y, Voss C, Shemesh T, Li Z, Coughlin M, Kozlov MM, Rapoport TA, Prinz WA(2008).Membrane proteins of the endoplasmic reticulum induce high-curvature tubules.Science, 319:1247-1250. [12]Krishna R, Karkute SG, Ansari WA, Jaiswal DK, Verma JP, Singh M(2019).Transgenic tomatoes for abiotic stress tolerance: status and way ahead.Biotech, 9:1-14. [13]Letunic I, Bork P (2021).Interactive Tree Of Life (iTOL) v5: an online tool for phylogenetic tree display and annotation. Nucleic acids research 49, W293-W296.., :-. [14]Li Q, Shen H, Yuan S, Dai X, Yang C (2023).miRNAs and lncRNAs in tomato: Roles in biotic and abiotic stress responses. Frontiers in Plant Science 13, 1094459.., :-. [15]Liang M, Li H, Zhou F, Li H, Liu J, Hao Y, Wang Y, Zhao H, Han S(2015).Subcellular distribution of NTL transcription factors in Arabidopsis thaliana.Traffic, 16:1062-1074. [16]Livak KJ, Schmittgen TD(2001).Analysis of relative gene expression data using real-time quantitative PCR and the 2? ΔΔCT method.methods, 25:402-408. [17]Lu P-L(2013).Physiological functional analysis of a stress-induced protein,HVA22,in Escherichia coli.Access Intternational Journals, 1:14-23. [18]Meng F, Zhao Q, Zhao X, Yang C, Liu R, Pang J, Zhao W, Wang Q, Liu M, Zhang Z (2022).A rice protein modulates endoplasmic reticulum homeostasis and coordinates with a transcription factor to initiate blast disease resistance. Cell reports 39, ., :-. [19]Rombauts S, Déhais P, Van Montagu M, Rouzé P(1999).PlantCARE,a plant cis-acting regulatory element database.Nucleic acids research, 27:295-296. [20]Sharon K, Suvarna S(2017).Cloning of HVA22 homolog from Aloe vera and preliminary study of transgenic plant development.Int. J. Pure App. Biosci, 5:1113-1121. [21]Shen Q, Chen C-N, Brands A, Pan S-M, Tuan-Hua DH(2001).The stress-and abscisic acid-induced barley gene HVA22: developmental regulation and homologues in diverse organisms.Plant molecular biology, 45:327-340. [22]Shen Q, Uknes S, Ho T(1993).Hormone response complex in a novel abscisic acid and cycloheximide-inducible barley gene.Journal of Biological Chemistry, 268:23652-23660. [23]Sun H, Li J, Li X, Lv Q, Chen L, Wang B, Li L(2022).RING E3 ubiquitin ligase TaSADR1 negatively regulates drought resistance in transgenic Arabidopsis.Plant Physiology and Biochemistry, 170:255-265. [24]Tamura K, Stecher G, Kumar S(2021).MEGA11: molecular evolutionary genetics analysis version 11.Molecular biology and evolution, 38:3022-3027. [25]Wagih O(2017).ggseqlogo: a versatile R package for drawing sequence logos.Bioinformatics, 33:3645-3647. [26]Wai AH, Waseem M, Cho L-H, Kim S-T, Lee D-j, Kim C-K, Chung M-Y (2022).Comprehensive genome-wide analysis and expression pattern profiling of the SlHVA22 gene family unravels their likely involvement in the abiotic stress adaptation of tomato. International Journal of Molecular Sciences 23, 12222.., :-. [27]Wang M, Yuan J, Qin L, Shi W, Xia G, Liu S(2020).Ta CYP 81D5,one member in a wheat cytochrome P450 gene cluster,confers salinity tolerance via reactive oxygen species scavenging.Plant biotechnology journal, 18:791-804. [28]Wang Y, Cao X, Zhang D, Li Y, Wang Q, Ma F, Xu X, Zhan X, Hu T (2023).SlGATA17, A tomato GATA protein, interacts with SlHY5 to modulate salinity tolerance and germination. Environmental and Experimental Botany 206, 105191.., :-. [29]Xiong H, Yu J, Miao J, Li J, Zhang H, Wang X, Liu P, Zhao Y, Jiang C, Yin Z(2018).Natural variation in OsLG3 increases drought tolerance in rice by inducing ROS scavenging.Plant Physiology, 178:451-467. [30]Yang Y-G, Lv W-T, Li M-J, Wang B, Sun D-M, Deng X(2013).Maize membrane-bound transcription factor Zmbzip17 is a key regulator in the cross-talk of ER quality control and ABA signaling.Plant and cell physiology, 54:2020-2033. [31]Zhang H, Yuan Y, Xing H, Xin M, Saeed M, Wu Q, Wu J, Zhuang T, Zhang X, Mao L (2023).Genome-wide identification and expression analysis of the HVA22 gene family in cotton and functional analysis of GhHVA22E1D in drought and salt tolerance. Frontiers in Plant Science 14, 1139526.., :-. [32]Zhao L, Wang B, Yang T, Yan H, Yu Q, Wang J (2023).Genome-wide identification and analysis of the evolution and expression pattern of the HVA22 gene family in three wild species of tomatoes. PeerJ 11, e14844.., :-. [33]Zhou P, An Y, Wang Z, Du H, Huang B (2014).Characterization of gene expression associated with drought avoidance and tolerance traits in a perennial grass species. PLoS One 9, e103611.., :-. |
[1] | 廖人玉, 王佳伟. 从损伤到重生——REF1小肽如何激发植物的内在再生潜能[J]. 植物学报, 2024, 59(3): 347-350. |
[2] | 段政勇, 丁敏, 王宇卓, 丁艺冰, 陈凌, 王瑞云, 乔治军. 糜子SBP基因家族全基因组鉴定及表达分析[J]. 植物学报, 2024, 59(2): 231-244. |
[3] | 蔡淑钰, 刘建新, 王国夫, 吴丽元, 宋江平. 褪黑素促进镉胁迫下番茄种子萌发的调控机理[J]. 植物学报, 2023, 58(5): 720-732. |
[4] | 吴楠, 覃磊, 崔看, 李海鸥, 刘忠松, 夏石头. 甘蓝型油菜EXA1的克隆及其对植物抗病的调控作用[J]. 植物学报, 2023, 58(3): 385-393. |
[5] | 王菲菲, 周振祥, 洪益, 谷洋洋, 吕超, 郭宝健, 朱娟, 许如根. 大麦NF-YC基因鉴定及在盐胁迫下的表达分析[J]. 植物学报, 2023, 58(1): 140-149. |
[6] | 郭书亚, 艾金祥, 陈虹宇, 邵烨瑶, 汪妍, 王倩, 叶怡彤, 张雅婷, 丁哲晓, 吴昊辰, 吴玉环, 张建新, 饶米德, 刘鹏. 基于主成分-聚类-逐步回归分析构建番茄苗期耐铝性综合评价体系[J]. 植物学报, 2022, 57(4): 479-489. |
[7] | 王晓敏, 李洪磊, 王林, 周鹏泽, 白圣懿, 李国花, 郑福顺, 陶小荣, 程国新, 高艳明, 李建设. 银川番茄斑萎病毒的分子鉴定[J]. 植物学报, 2021, 56(6): 715-721. |
[8] | 范凯, 叶方婷, 毛志君, 潘鑫峰, 李兆伟, 林文雄. 被子植物小热激蛋白家族的比较基因组学分析[J]. 植物学报, 2021, 56(3): 245-261. |
[9] | 谢露露, 崔青青, 董春娟, 尚庆茂. 植物嫁接愈合分子机制研究进展[J]. 植物学报, 2020, 55(5): 634-643. |
[10] | 范业赓,丘立杭,黄杏,周慧文,甘崇琨,李杨瑞,杨荣仲,吴建明,陈荣发. 甘蔗节间伸长过程赤霉素生物合成关键基因的表达及相关植物激素动态变化[J]. 植物学报, 2019, 54(4): 486-496. |
[11] | 王小龙,刘凤之,史祥宾,王孝娣,冀晓昊,王志强,王宝亮,郑晓翠,王海波. 葡萄NCED基因家族进化及表达分析[J]. 植物学报, 2019, 54(4): 474-485. |
[12] | 化文平,陈尘,智媛,刘莉,王喆之,李翠芹. SmGGPPS2对丹参酮合成的影响[J]. 植物学报, 2019, 54(2): 217-226. |
[13] | 刘铭, 刘霞, 孙然, 李玉灵, 杜克久. 多氯联苯促进毛白杨不定根分化的效应[J]. 植物学报, 2018, 53(6): 764-772. |
[14] | 张天鹏, 杨兴洪. 番茄果实早期发育的分子生理机制研究进展[J]. 植物学报, 2018, 53(6): 856-866. |
[15] | 马爱民, 漆小泉. 利用多组学手段解析番茄育种过程中代谢物变化的机制[J]. 植物学报, 2018, 53(5): 578-580. |
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||