植物学报 ›› 2024, Vol. 59 ›› Issue (4): 0-0.DOI: 10.11983/CBB23129

• 研究报告 •    

SlHVA22l基因调节番茄耐旱性研究

赵来鹏1, 2, 王柏柯2, 杨涛2, 李宁2, 杨海涛2, 王娟2*, 闫会转1*




  

  1. 1新疆农业大学园艺学院, 乌鲁木齐 830052;2新疆农业科学院园艺作物研究所, 新疆特色果蔬基因组研究与遗传改良重点研究室, 乌鲁木齐 830091

  • 收稿日期:2023-09-15 修回日期:2023-12-15 出版日期:2024-07-01 发布日期:2024-01-12
  • 通讯作者: 王娟, 闫会转
  • 基金资助:

    农业科技创新稳定支持项目(No.xjnkywdzc-2023001)、自治区重点研发计划项目(No.2022B02032-2)、新疆特色果蔬基因组研究与遗传改良重点实验室开放课题(2023)和新疆维吾尔自治区蔬菜产业技术体系

Investigation of SlHVA22l gene regulating drought tolerance in tomato

Laipeng Zhao1,2, Baike Wang2, Tao Yang2, Ning Li2, Haitao Yang2, Juan Wang2*, Huizhuan Yan1* #br#

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  • Received:2023-09-15 Revised:2023-12-15 Online:2024-07-01 Published:2024-01-12
  • Contact: Juan Wang,Huizhuan Yan

摘要: 植物在生长发育过程中面临周围环境带来的各种非生物胁迫。其中干旱胁迫严重影响作物生长, 降低其产量。在植物中具有以TB2/DP1结构域为特征的HVA22蛋白参与调控植物的生长发育和非生物胁迫响应, 然而在番茄干旱胁迫响应中的功能并不清楚。因此, 在本研究中, 我们探索了番茄SlHVA22l基因的功能。研究表明, 番茄SlHVA22l与其他双子叶植物中的HVA22l同源蛋白具有较高的序列相似性, 且SlHVA22l基因表达受干旱胁迫和植物激素(ABA和MeJA)诱导。同时, 通过酵母(Saccharomyces cerevisiae)异源表达初步探明了SlHVA22l基因的抗旱功能。此外, 通过VIGS技术沉默番茄内源SlHVA22l基因的植株对干旱敏感。沉默植株经干旱处理后表现出较高的H2O2和MDA含量, 以及较低的O2-清除率, 且沉默植株中的SOD、POD、CAT和APX活性较对照植株均显著降低。综上所述, 这些结果表明SlHVA22l基因在番茄抵御干旱胁迫过程中发挥了重要作用。

关键词: 番茄, SlHVA22,  干旱胁迫, VIGS, 基因表达

Abstract: Plants face inevitably encounter a multitude of environmental stresses during the growth and development stages. Among them, drought stress significantly hampers crop growth and reduces yield. Plant HVA22 protein characterized by the TB2/DP1 structural domain and implicate in the modulation of plant growth, development, and responses to abiotic stress. However, its precise function in the context of drought stress response in tomato remains to be elucidated. Therefore, in this study, we investigated the functional role of the tomato SlHVA22l gene in drought tolerance. The results showed that amino acid of SlHVA22l exhibits a higher degree of sequence similarity to HVA22l homologous proteins found in other dicotyledonous plants. Furthermore, the expression pattern analysis revealed a significant up-regulation of the SlHVA22l gene in response to drought stress and phytohormones (ABA and MeJA). Moreover, the drought tolerance function of the SlHVA22l gene was subsequently initially verify by yeast heterologous expression and silencing the endogenous SlHVA22l gene in tomato through VIGS. The silenced plants exhibited higher H2O2 and MDA contents, as well as lower O2- scavenging after drought treatment. Meanwhile, the activities of SOD, POD, CAT, and APX were significantly decreased in silenced plants compared to control plants. Collectively, these results indicate that the SlHVA22l gene plays an important role in tomato resistance to drought stress.

Key words: tomato,  SlHVA22, drought stress,  VIGS,  gene expression