亲免素在植物体内的功能研究进展

doi:10.11983/CBB16202

摘要/Abstract

摘要： 亲免素(immunophilin)是一种免疫抑制剂受体, 广泛存在于细菌、病毒、真菌、植物和动物等生物体中。植物体内亲免素由FK506结合蛋白(FKBPs)、亲环素(CYPs)和parvulin蛋白构成。大多数亲免素具有肽基脯氨酰顺反异构酶活性, 可以作为分子伴侣指导蛋白质的正确折叠。该文总结了亲免素在激素信号传递、光合作用、胁迫响应及基因表达等方面的最新研究进展, 并对今后该领域的研究进行了展望。

关键词: 生长发育, 基因表达, 亲免素, 光合作用, 胁迫响应

Abstract: Immunophilins are receptors for immunosuppressive drugs that exist widely in bacteria, viruses, fungi, plants and animals. In plants, immunophilins have three subfamilies: FK506-binding proteins, cyclosporine A-binding proteins (cyclophilins) and parvulins. Many members of immunophilins are peptidyl-prolyl cis-trans isomerases that may act as molecular chaperones for protein folding. In this review, we summarize the research progress in the functions of plant immunophilins in hormone signaling, photosynthesis, stress response and gene expression etc., to provide valuable information for further study of immunophilin functions.

Key words: growth and development, gene expression, immunophilins, photosynthesis, stress response

孙万梅, 王晓珠, 韩二琴, 韩丽, 孙丽萍, 彭再慧, 王邦俊. 亲免素在植物体内的功能研究进展. 植物学报, 2017, 52(6): 808-819.

Sun Wanmei, Wang Xiaozhu, Han Erqin, Han Li, Sun Liping, Peng Zaihui, Wang Bangjun. Advances in the Functions of Immunophilins in Plants. Chinese Bulletin of Botany, 2017, 52(6): 808-819.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB16202

https://www.chinbullbotany.com/CN/Y2017/V52/I6/808

图/表 2

表1 部分植物亲免素在细胞内的定位及功能

Table 1 The localization and function of some immunophilins in plant cells

图1 亲免素与相关蛋白质相互作用调节下游信号的传递 (A) ABCB蛋白在TWD1的帮助下正确定位于细胞膜, 将细胞质内的生长素运出; 这个过程可能受BR (brassinosteroid)的影响(Geisler and Bailly, 2007; Wu et al., 2010); (B) 在BR的作用下, TWD1与BRI1和BAK1相互作用促进其磷酸化, 抑制BIN2的活性,影响BES1/BZR1的磷酸化, 从而调节BR响应基因的表达(Zhao et al., 2016; Chaiwanon et al., 2016); (C) AtCYP20-2与BZR1相互作用改变BZR1的构象, 调节FLD的表达, 改变开花时间(Zhang et al., 2013); (D) 在有生长素时, LRT2与OsIAA11相互作用, 使OsIAA11构象发生变化, 促进其与OsTIR1相互作用, 形成OsTIR1-OsIAA11复合体。在OsTIR1-IAA11复合体的帮助下泛素分子连接到OsIAA11上, 然后OsIAA11蛋白被26S蛋白酶体降解, ARFs形成有活性的形式, 从而激活生长素调节基因的表达(Jing et al.,2015)。

Figure 1 Immunophilins regulate downstream signaling by interacting with related proteins(A) TWD1 helps the correct secretion of ABCB transporters to the plasma membrane, thus regulating ABCB-mediated auxin transport; Brassinosteroid might affect this process (Geisler and Bailly, 2007; Wu et al., 2010); (B) TWD1 physically interacts with BRI1 and BAK1 in a BR-independent manner, which may facilitate the phosphorylation of BRI1 and BAK1, inhibit BIN2 activity,and affect BES1/BZR1 phosphorylation, eventually regulate the BR response gene expression (Zhao et al., 2016; Chaiwanon et al., 2016); (C) A conformational change of BZR1 mediated by AtCYP20-2 causes altered flowering time through the modulation of FLD expression (Zhang et al., 2013); (D) In the presence of auxin, OsIAA11 conformation was changed by interacting with LRT2, and then formed the OsTIR1-OsIAA11 complex. OsTIR1-OsIAA11 complex facilitated OsIAA11 binding to 26S proteasome and caused OsIAA11 degradation; Finally, ARFs became active and activated auxin-regulated genes (Jing et al., 2015).

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